Gene/Proteome Database (LMPD)

LMPD ID
LMP007702
Gene ID
947388
Species
Escherichia coli K-12 (E. coli)
Gene Name
2-octaprenyl-6-methoxyphenol hydroxylase, FAD/NAD(P)-binding
Gene Symbol
ubiH
Synonyms
ECK2902; JW2875; acd; visB
Summary
visC, ubiH, ubiF and mhpA are paralogs. [More information is available at EcoGene: EG11324]. ubiH is thought to encode 2-octaprenyl-6-methoxyphenol hydroxylase, one of the aerobic hydroxylases in the ubiquinone biosynthesis pathway. [More information is available at EcoCyc: EG11324].
Orthologs
View orthologs and multiple alignments for ubiH

Proteins

2-octaprenyl-6-methoxyphenol hydroxylase, FAD/NAD(P)-binding [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_417383
Protein GI 16130809
UniProt ID P25534
Length 392
RefSeq Status REVIEWED
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR

Gene Information

Entrez Gene ID
947388
Gene Name
2-octaprenyl-6-methoxyphenol hydroxylase, FAD/NAD(P)-binding
Gene Symbol
ubiH
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0008681 IMP:EcoCyc F 2-octaprenyl-6-methoxyphenol hydroxylase activity
GO:0050660 IEA:InterPro F flavin adenine dinucleotide binding
GO:0016709 IEA:InterPro F oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0009416 IMP:EcoliWiki P response to light stimulus
GO:0006979 IMP:EcoliWiki P response to oxidative stress
GO:0006744 IMP:EcoCyc P ubiquinone biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
eco01100 Metabolic pathways
eco00130 Ubiquinone and other terpenoid-quinone biosynthesis
ko00130 Ubiquinone and other terpenoid-quinone biosynthesis
M00117 Ubiquinone biosynthesis, prokaryotes, chorismate => ubiquinone
eco_M00117 Ubiquinone biosynthesis, prokaryotes, chorismate => ubiquinone

BIOCYC Pathway Links

BIOCYC Pathway ID Description
ALL-CHORISMATE-PWY superpathway of chorismate metabolism
ALL-CHORISMATE-PWY superpathway of chorismate metabolism
UBISYN-PWY superpathway of ubiquinol-8 biosynthesis (prokaryotic)
UBISYN-PWY superpathway of ubiquinol-8 biosynthesis (prokaryotic)
PWY-6708 ubiquinol-8 biosynthesis (prokaryotic)
PWY-6708 ubiquinol-8 biosynthesis (prokaryotic)

Domain Information

InterPro Annotations

Accession Description
IPR003042 Aromatic-ring hydroxylase-like
IPR002938 Monooxygenase, FAD-binding
IPR011295 UbiH
IPR010971 Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/UbiI/COQ6
IPR018168 Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6, conserved site

UniProt Annotations

Entry Information

Gene Name
2-octaprenyl-6-methoxyphenol hydroxylase, FAD/NAD(P)-binding
Protein Entry
UBIH_ECOLI
UniProt ID
P25534
Species
E. coli

Comments

Comment Type Description
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
Disruption Phenotype Cells lacking this gene are photosensitive and are not able to grow with a nonfermentable source of carbon. They also fail to produce ubiquinone, and accumulate 2- octaprenylphenol and 2-octaprenyl-6-methoxyphenol. {ECO:0000269|PubMed:1339425, ECO:0000269|PubMed:4572721}.
Function Is likely an oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinol. {ECO:0000269|PubMed:4572721}.
Pathway Cofactor biosynthesis; ubiquinone biosynthesis.
Similarity Belongs to the UbiH/COQ6 family. {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007702 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16130809 RefSeq NP_417383 392 2-octaprenyl-6-methoxyphenol hydroxylase, FAD/NAD(P)-binding [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007702 proteins

Reference Database Accession Length Protein Name
GI:16130809 GenBank KHI14490.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]
GI:16130809 GenBank KHI16083.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]
GI:16130809 GenBank KHI62532.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]
GI:16130809 GenBank KHI85925.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]
GI:16130809 GenBank KHI94648.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]
GI:16130809 gnl IGS 392 2-octaprenyl-6-methoxyphenol hydroxylase, FAD/NAD(P)-binding [Escherichia coli ER2796]

Related Sequences to LMP007702 proteins

Reference Database Accession Length Protein Name
GI:16130809 DBBJ BAA14326.1 392 UbiH [Escherichia coli]
GI:16130809 EMBL CBJ02602.1 392 2-octaprenyl-6-methoxyphenol hydroxylase [Escherichia coli ETEC H10407]
GI:16130809 GenBank KEL03425.1 392 2-polyprenyl-6-methoxyphenol 4-hydroxylase [Escherichia coli 4-203-08_S3_C3]
GI:16130809 RefSeq YP_006116647.1 392 2-octaprenyl-6-methoxyphenol hydroxylase [Escherichia coli ETEC H10407]
GI:16130809 RefSeq WP_000111196.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]
GI:16130809 RefSeq WP_032258336.1 392 2-octaprenyl-6-methoxyphenyl hydroxylase [Escherichia coli]