Gene/Proteome Database (LMPD)

LMPD ID
LMP007696
Gene ID
946213
Species
Escherichia coli K-12 (E. coli)
Gene Name
short chain acyl-CoA synthetase, anaerobic
Gene Symbol
fadK
Synonyms
ECK1699; JW5910; ydiD
Summary
AFMB Structural Genomics target Number 54 (http://afmb.cnrs-mrs.fr/article171.html). [More information is available at EcoGene: EG12357]. FadK is an acyl-CoA synthetase that is primarily active on short chain fatty acids and acts in anaerobic -oxidation of fatty acids . [More information is available at EcoCyc: EG12357].
Orthologs
View orthologs and multiple alignments for fadK

Proteins

short chain acyl-CoA synthetase, anaerobic [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_416216
Protein GI 145698273
UniProt ID P38135
Length 548
RefSeq Status REVIEWED
MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVVDNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKLAPATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMSDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTTSGKIQKFLLRKDIMRRLTQDVCEEIE

Gene Information

Entrez Gene ID
946213
Gene Name
short chain acyl-CoA synthetase, anaerobic
Gene Symbol
fadK
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0031956 IDA:EcoCyc F medium-chain fatty acid-CoA ligase activity
GO:0019395 IGI:EcoCyc P fatty acid oxidation

BIOCYC Pathway Links

BIOCYC Pathway ID Description
FAO-PWY fatty acid beta-oxidation I
FAO-PWY fatty acid beta-oxidation I

Domain Information

InterPro Annotations

Accession Description
IPR025110 AMP-binding enzyme C-terminal domain
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
short chain acyl-CoA synthetase, anaerobic
Protein Entry
FADK_ECOLI
UniProt ID
P38135
Species
E. coli

Comments

Comment Type Description
Catalytic Activity ATP + a short-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Function Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is maximally active on C6 and C8 substrates.
Induction Expression independent of FadR. The levels of expression vary with the terminal electron acceptor (nitrate, fumarate). FadK is not expressed under aerobic growth conditions and it could functionally replace FadD under anaerobic conditions.
Sequence Caution Sequence=BAA15470.2; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=M69116; Type=Frameshift; Positions=Several; Evidence={ECO:0000305};
Similarity Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location Membrane; Peripheral membrane protein. Note=Partially membrane-associated.
Subunit Homodimer. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007696 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
145698273 RefSeq NP_416216 548 short chain acyl-CoA synthetase, anaerobic [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007696 proteins

Reference Database Accession Length Protein Name
GI:145698273 GenBank KHH72270.1 548 short chain acyl-CoA synthetase [Escherichia coli]
GI:145698273 GenBank KHH96260.1 548 short chain acyl-CoA synthetase [Escherichia coli]
GI:145698273 GenBank KHI16032.1 548 short chain acyl-CoA synthetase [Escherichia coli]
GI:145698273 GenBank KHI17893.1 548 short chain acyl-CoA synthetase [Escherichia coli]
GI:145698273 GenBank KHI61212.1 548 short chain acyl-CoA synthetase [Escherichia coli]
GI:145698273 gnl IGS 548 short chain acyl-CoA synthetase, anaerobic [Escherichia coli ER2796]

Related Sequences to LMP007696 proteins

Reference Database Accession Length Protein Name
GI:145698273 DBBJ BAL38627.1 566 short chain acyl-CoA synthetase, anaerobic [Escherichia coli str. K-12 substr. MDS42]
GI:145698273 GenBank ABZ26910.1 566 Sequence 848 from patent US 7314974
GI:145698273 GenBank EGU28796.1 566 short chain acyl-CoA synthetase [Escherichia coli XH140A]
GI:145698273 GenBank EGV49475.1 566 short chain acyl-CoA synthetase [Escherichia coli XH001]
GI:145698273 gnl REF_ecoli 566 short chain acyl-CoA synthetase [Escherichia coli str. K-12 substr. DH10B]
GI:145698273 RefSeq YP_007557020.1 566 short chain acyl-CoA synthetase, anaerobic [Escherichia coli str. K-12 substr. MDS42]