Gene/Proteome Database (LMPD)

LMPD ID
LMP007630
Gene ID
944854
Species
Escherichia coli K-12 (E. coli)
Gene Name
dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
Gene Symbol
lpd
Synonyms
ECK0115; JW0112; dhl; lpdA
Summary
Holoenzyme is AceE(24)+AceF(24)+Lpd(12). [More information is available at EcoGene: EG10543]. Lipoamide dehydrogenase is the E3 component of three multicomponent enzyme complexes: |FRAME: [More information is available at EcoCyc: EG10543].
Orthologs
View orthologs and multiple alignments for lpd

Proteins

dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_414658
Protein GI 16128109
UniProt ID P0A9P0
Length 474
RefSeq Status REVIEWED
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK

Gene Information

Entrez Gene ID
944854
Gene Name
dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
Gene Symbol
lpd
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:EcoliWiki C cytoplasm
GO:0005829 IDA:UniProtKB C cytosol
GO:0016020 IDA:UniProtKB C membrane
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0004148 IDA:EcoliWiki F dihydrolipoyl dehydrogenase activity
GO:0015036 IDA:EcoliWiki F disulfide oxidoreductase activity
GO:0050660 IDA:EcoliWiki F flavin adenine dinucleotide binding
GO:0042802 IPI:IntAct F identical protein binding
GO:0008270 IDA:EcoliWiki F zinc ion binding
GO:0006103 IMP:EcoliWiki P 2-oxoglutarate metabolic process
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0019464 IMP:EcoCyc P glycine decarboxylation via glycine cleavage system
GO:0006096 IEA:UniProtKB-KW P glycolytic process
GO:0055114 IDA:EcoliWiki P oxidation-reduction process
GO:0006090 IDA:EcoliWiki P pyruvate metabolic process
GO:0006979 IMP:EcoCyc P response to oxidative stress

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
eco01200 Carbon metabolism
ko01200 Carbon metabolism
eco00020 Citrate cycle (TCA cycle)
ko00020 Citrate cycle (TCA cycle)
M00009 Citrate cycle (TCA cycle, Krebs cycle)
eco_M00009 Citrate cycle (TCA cycle, Krebs cycle)
M00011 Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate
eco_M00011 Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate
eco00260 Glycine, serine and threonine metabolism
ko00260 Glycine, serine and threonine metabolism
eco00010 Glycolysis / Gluconeogenesis
ko00010 Glycolysis / Gluconeogenesis
eco01100 Metabolic pathways
eco01120 Microbial metabolism in diverse environments
eco00620 Pyruvate metabolism
ko00620 Pyruvate metabolism
M00307 Pyruvate oxidation, pyruvate => acetyl-CoA
eco_M00307 Pyruvate oxidation, pyruvate => acetyl-CoA
eco00280 Valine, leucine and isoleucine degradation
ko00280 Valine, leucine and isoleucine degradation

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5084 2-oxoglutarate decarboxylation to succinyl-CoA
PWY-5084 2-oxoglutarate decarboxylation to succinyl-CoA
TCA TCA cycle
TCA TCA cycle I (prokaryotic)
TCA TCA cycle I (prokaryotic)
PYRUVDEHYD-PWY acetyl-CoA biosynthesis (from pyruvate)
PWY-2201 folate transformations I
PWY-2201 folate transformations I
PWY-3841 folate transformations II (plants)
1CMET2-PWY formylTHF biosynthesis
1CMET2-PWY formylTHF biosynthesis
1CMET2-PWY formylTHF biosynthesis I
GLYCINE-SYN2-PWY glycine biosynthesis II
GLYCLEAV-PWY glycine cleavage
GLYCLEAV-PWY glycine cleavage
PWY-7218 photosynthetic 3-hydroxybutyrate biosynthesis
PYRUVDEHYD-PWY pyruvate decarboxylation to acetyl CoA
PYRUVDEHYD-PWY pyruvate decarboxylation to acetyl CoA
PWY-5482 pyruvate fermentation to acetate II
P41-PWY pyruvate fermentation to acetate and lactate I
ANARESP1-PWY respiration (anaerobic)
ANARESP1-PWY respiration (anaerobic)
ANARESP1-PWY respiration (anaerobic)
GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA

Domain Information

InterPro Annotations

Accession Description
IPR006258 Dihydrolipoamide dehydrogenase
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase
IPR016156 FAD/NAD-linked reductase, dimerisation domain
IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain
IPR012999 Pyridine nucleotide-disulphide oxidoreductase, class I, active site
IPR004099 Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain

UniProt Annotations

Entry Information

Gene Name
dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
Protein Entry
DLDH_ECOLI
UniProt ID
P0A9P0
Species
E. coli

Comments

Comment Type Description
Catalytic Activity Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH.
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250}; Note=Binds 1 FAD per subunit. {ECO:0000250};
Function Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes.
Interaction Self; NbExp=2; IntAct=EBI-542856, EBI-542856; P06959:aceF; NbExp=3; IntAct=EBI-542856, EBI-542707; P03018:uvrD; NbExp=2; IntAct=EBI-542856, EBI-559573;
Miscellaneous The active site is a redox-active disulfide bond.
Sequence Caution Sequence=CAA24742.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Similarity Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:16079137}. Cell inner membrane {ECO:0000269|PubMed:16079137}; Peripheral membrane protein {ECO:0000269|PubMed:16079137}.
Subunit Homodimer.

Identical and Related Proteins

Unique RefSeq proteins for LMP007630 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16128109 RefSeq NP_414658 474 dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007630 proteins

Reference Database Accession Length Protein Name
GI:16128109 GenBank KHI97567.1 474 dihydrolipoamide dehydrogenase [Escherichia coli]
GI:16128109 GenBank KHJ05962.1 474 dihydrolipoamide dehydrogenase [Escherichia coli]
GI:16128109 GenBank KHJ15935.1 474 dihydrolipoamide dehydrogenase [Escherichia coli]
GI:16128109 GenBank KHJ21543.1 474 dihydrolipoamide dehydrogenase [Escherichia coli]
GI:16128109 GenBank KHJ27055.1 474 dihydrolipoamide dehydrogenase [Escherichia coli]
GI:16128109 gnl IGS 474 lipoamide dehydrogenase, E3 component is part of three enzyme complexes [Escherichia coli ER2796]

Related Sequences to LMP007630 proteins

Reference Database Accession Length Protein Name
GI:16128109 GenBank EDU31664.1 495 dihydrolipoamide dehydrogenase [Escherichia coli O157:H7 str. EC4196]
GI:16128109 GenBank EDU51742.1 495 dihydrolipoamide dehydrogenase [Escherichia coli O157:H7 str. EC4113]
GI:16128109 GenBank EDU69398.1 495 dihydrolipoamide dehydrogenase [Escherichia coli O157:H7 str. EC4076]
GI:16128109 GenBank EDU75478.1 495 dihydrolipoamide dehydrogenase [Escherichia coli O157:H7 str. EC4401]
GI:16128109 gnl tigr 495 dihydrolipoamide dehydrogenase [Escherichia coli SMS-3-5]
GI:16128109 gnl REF_tigr 495 dihydrolipoamide dehydrogenase [Escherichia coli SMS-3-5]