Gene/Proteome Database (LMPD)

LMPD ID
LMP007603
Gene ID
949097
Species
Escherichia coli K-12 (E. coli)
Gene Name
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Gene Symbol
fadJ
Synonyms
ECK2335; JW2338; yfcX
Summary
Also known as beta-oxidation complex II. [More information is available at EcoGene: EG14127]. During anaerobic beta-oxidation of fatty acids FadI, FadJ, and FadK serve functions parallel to those of FadA, FadB, and FadD in the aerobic pathway . [More information is available at EcoCyc: G7212].
Orthologs
View orthologs and multiple alignments for fadJ

Proteins

enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_416843
Protein GI 16130274
UniProt ID P77399
Length 714
RefSeq Status REVIEWED
MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ

Gene Information

Entrez Gene ID
949097
Gene Name
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Gene Symbol
fadJ
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-HAMAP C cytoplasm
GO:0003857 IEA:UniProtKB-HAMAP F 3-hydroxyacyl-CoA dehydrogenase activity
GO:0008692 IEA:UniProtKB-HAMAP F 3-hydroxybutyryl-CoA epimerase activity
GO:0051287 IEA:InterPro F NAD binding
GO:0004300 IEA:UniProtKB-HAMAP F enoyl-CoA hydratase activity
GO:0006635 IEA:UniProtKB-UniPathway P fatty acid beta-oxidation

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
eco01040 Biosynthesis of unsaturated fatty acids
ko01040 Biosynthesis of unsaturated fatty acids
eco00650 Butanoate metabolism
ko00650 Butanoate metabolism
eco00930 Caprolactam degradation
ko00930 Caprolactam degradation
eco01200 Carbon metabolism
ko01200 Carbon metabolism
eco00071 Fatty acid degradation
ko00071 Fatty acid degradation
eco01212 Fatty acid metabolism
ko01212 Fatty acid metabolism
eco00281 Geraniol degradation
ko00281 Geraniol degradation
eco00903 Limonene and pinene degradation
ko00903 Limonene and pinene degradation
eco00310 Lysine degradation
ko00310 Lysine degradation
eco01100 Metabolic pathways
eco01120 Microbial metabolism in diverse environments
eco00640 Propanoate metabolism
ko00640 Propanoate metabolism
eco00380 Tryptophan metabolism
ko00380 Tryptophan metabolism
eco00280 Valine, leucine and isoleucine degradation
ko00280 Valine, leucine and isoleucine degradation
eco00410 beta-Alanine metabolism
ko00410 beta-Alanine metabolism
M00087 beta-Oxidation
eco_M00087 beta-Oxidation

BIOCYC Pathway Links

BIOCYC Pathway ID Description
FAO-PWY fatty acid beta-oxidation I
FAO-PWY fatty acid beta-oxidation I
PWY-5137 fatty acid beta-oxidation III (unsaturated, odd number)
PWY-7007 methyl ketone biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding
IPR006180 3-hydroxyacyl-CoA dehydrogenase, conserved site
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like
IPR029045 ClpP/crotonase-like domain
IPR001753 Crotonase_core_superfam
IPR013328 Dehydrogenase, multihelical
IPR012802 FadJ
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Protein Entry
FADJ_ECOLI
UniProt ID
P77399
Species
E. coli

Comments

Comment Type Description
Catalytic Activity (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
Catalytic Activity (S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA.
Function Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids. {ECO:0000269|PubMed:12270828, ECO:0000269|PubMed:12535077}.
Induction Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein.
Pathway Lipid metabolism; fatty acid beta-oxidation.
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}.
Similarity In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000250}.
Subunit Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007603 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16130274 RefSeq NP_416843 714 enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007603 proteins

Reference Database Accession Length Protein Name
GI:16130274 GenBank KHD59173.1 714 multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli]
GI:16130274 GenBank KHH68448.1 714 multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli]
GI:16130274 GenBank KHH89814.1 714 multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli]
GI:16130274 GenBank KHI21516.1 714 multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli]
GI:16130274 GenBank KHI60669.1 714 multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli]
GI:16130274 gnl IGS 714 fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli ER2796]

Related Sequences to LMP007603 proteins

Reference Database Accession Length Protein Name
GI:16130274 GenBank ENF75972.1 714 fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.10]
GI:16130274 GenBank ENF85935.1 714 fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.12]
GI:16130274 GenBank ENF90539.1 714 fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.13]
GI:16130274 GenBank ENF96306.1 714 fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.15]
GI:16130274 GenBank ENG02945.1 714 fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.3]
GI:16130274 GenBank ENG12373.1 714 fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.5]