Gene/Proteome Database (LMPD)

LMPD ID
LMP007594
Gene ID
945863
Species
Escherichia coli K-12 (E. coli)
Gene Name
phosphatidylglycerophosphatase B
Gene Symbol
pgpB
Synonyms
ECK1273; JW1270
Summary
The pgpB gene is nonessential, but is synthetically lethal with a bacA ybjG double mutant, due to its UppP activity. ybjG, pgpB, and lpxT are paralogs. [More information is available at EcoGene: EG10705]. PgpB is one of three phosphatidylglycerophosphatase (PGP-ase) activities in Escherichia coli. [More information is available at EcoCyc: EG10705].
Orthologs
View orthologs and multiple alignments for pgpB

Proteins

phosphatidylglycerophosphatase B [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415794
Protein GI 16129239
UniProt ID P0A924
Length 254
RefSeq Status REVIEWED
MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWVTETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQEPRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFPSGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLISWALVAVATWLAQRICGPLTPPAEENREIAQREQES

Gene Information

Entrez Gene ID
945863
Gene Name
phosphatidylglycerophosphatase B
Gene Symbol
pgpB
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009279 IMP:EcoCyc C cell outer membrane
GO:0005887 IDA:EcoCyc C integral component of plasma membrane
GO:0005886 IMP:EcoCyc C plasma membrane
GO:0000810 IEA:UniProtKB-EC F diacylglycerol diphosphate phosphatase activity
GO:0008195 IEA:UniProtKB-EC F phosphatidate phosphatase activity
GO:0008962 IMP:EcoCyc F phosphatidylglycerophosphatase activity
GO:0050380 IDA:EcoCyc F undecaprenyl-diphosphatase activity
GO:0016311 IMP:GOC P dephosphorylation
GO:0046474 IMP:EcoCyc P glycerophospholipid biosynthetic process
GO:0009252 IMP:EcoCyc P peptidoglycan biosynthetic process
GO:0006655 IEA:UniProtKB-UniPathway P phosphatidylglycerol biosynthetic process
GO:0009395 IEA:UniProtKB-KW P phospholipid catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00564 Glycerophospholipid metabolism
ko00564 Glycerophospholipid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-5668 cardiolipin biosynthesis I
PWY-5668 cardiolipin biosynthesis I
PWY-5668 cardiolipin biosynthesis I
PWY0-1545 cardiolipin biosynthesis III
PWY0-1545 cardiolipin biosynthesis III
PWY4FS-7 phosphatidylglycerol biosynthesis I (plastidic)
PWY4FS-8 phosphatidylglycerol biosynthesis II (non-plastidic)
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I
PHOSLIPSYN-PWY phospholipid biosynthesis I

Domain Information

InterPro Annotations

Accession Description
IPR000326 Phosphatidic acid phosphatase type 2/haloperoxidase

UniProt Annotations

Entry Information

Gene Name
phosphatidylglycerophosphatase B
Protein Entry
PGPB_ECOLI
UniProt ID
P0A924
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=530 uM for undecaprenyl pyrophosphate {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025}; KM=96 uM for farnesyl pyrophosphate {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025}; KM=80 uM for diacylglycerol pyrophosphate {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025}; KM=1700 uM for phosphatidate {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025}; Vmax=2167 nmol/min/mg enzyme with diacylglycerol pyrophosphate as substrate {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025}; Vmax=313 nmol/min/mg enzyme with phosphatidate as substrate {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025}; pH dependence: Optimum pH is 6.5 for DGPP phosphatase activity and 6.5-7.5 for undecaprenyl pyrophosphate phosphatase activity. {ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:8940025};
Catalytic Activity 1,2-diacyl-sn-glycerol 3-diphosphate + H(2)O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate.
Catalytic Activity A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate.
Catalytic Activity Ditrans,octacis-undecaprenyl diphosphate + H(2)O = ditrans,octacis-undecaprenyl phosphate + phosphate.
Catalytic Activity Phosphatidylglycerophosphate + H(2)O = phosphatidylglycerol + phosphate.
Enzyme Regulation Inhibited by Mn(2+) ions.
Function Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Also has undecaprenyl pyrophosphate phosphatase activity, required for the biosynthesis of the lipid carrier undecaprenyl phosphate. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. The pattern of activities varies according to subcellular location, PGP phosphatase activity is higher in the cytoplasmic membrane, whereas PA and LPA phosphatase activities are higher in the outer membrane. Activity is independent of a divalent cation ion and insensitive to inhibition by N- ethylmaleimide. {ECO:0000269|PubMed:15778224, ECO:0000269|PubMed:18411271, ECO:0000269|PubMed:21148555, ECO:0000269|PubMed:8940025}.
Mass Spectrometry Mass=30151; Method=MALDI; Range=2-254; Note=Reported mass includes mass of a C-terminal His6 tag.; Evidence={ECO:0000269|PubMed:18411271};
Pathway Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.
Ptm The N-terminus is blocked.
Similarity Belongs to the PA-phosphatase related phosphoesterase family. {ECO:0000305}.
Subcellular Location Cell inner membrane {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000269|PubMed:15919996}. Cell outer membrane {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein {ECO:0000269|PubMed:15919996}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007594 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129239 RefSeq NP_415794 254 phosphatidylglycerophosphatase B [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007594 proteins

Reference Database Accession Length Protein Name
GI:16129239 GenBank KHJ06720.1 254 phosphatidylglycerophosphatase [Escherichia coli]
GI:16129239 GenBank KHJ13541.1 254 phosphatidylglycerophosphatase [Escherichia coli]
GI:16129239 GenBank KHJ17865.1 254 phosphatidylglycerophosphatase [Escherichia coli]
GI:16129239 GenBank KHJ21084.1 254 phosphatidylglycerophosphatase [Escherichia coli]
GI:16129239 GenBank KHJ26326.1 254 phosphatidylglycerophosphatase [Escherichia coli]
GI:16129239 gnl IGS 254 phosphatidylglycerophosphatase B [Escherichia coli ER2796]

Related Sequences to LMP007594 proteins

Reference Database Accession Length Protein Name
GI:16129239 GenBank EFW58685.1 254 Phosphatidylglycerophosphatase B [Shigella flexneri CDC 796-83]
GI:16129239 GenBank EGI99839.1 254 PAP2 superfamily protein [Shigella boydii 3594-74]
GI:16129239 GenBank EQW35906.1 254 phosphatidylglycerophosphatase B [Escherichia coli UMEA 3052-1]
GI:16129239 gnl mgcchina 254 non-essential phosphatidylglycerophosphate phosphatase, membrane bound [Shigella boydii Sb227]
GI:16129239 RefSeq WP_001256536.1 254 MULTISPECIES: phosphatidylglycerophosphatase B [Shigella]
GI:16129239 RefSeq WP_021553552.1 254 phosphatidylglycerophosphatase B [Escherichia coli]