Gene/Proteome Database (LMPD)

LMPD ID
LMP007574
Gene ID
945019
Species
Escherichia coli K-12 (E. coli)
Gene Name
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Gene Symbol
dxr
Synonyms
ECK0172; JW0168; ispC; yaeM
Summary
1-deoxy-D-xylulose 5-phosphate reductoisomerase (Dxr) catalyzes the first committed step in the methylerythritol phosphate pathway of isoprenoid biosynthesis. [More information is available at EcoCyc: EG12715].
Orthologs
View orthologs and multiple alignments for dxr

Proteins

1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_414715
Protein GI 16128166
UniProt ID P45568
Length 398
RefSeq Status REVIEWED
MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVEQCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIVGAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSLPQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTALNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKEVMRLAS

Gene Information

Entrez Gene ID
945019
Gene Name
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Gene Symbol
dxr
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0030604 IDA:EcoCyc F 1-deoxy-D-xylulose-5-phosphate reductoisomerase activity
GO:0070402 IDA:EcoCyc F NADPH binding
GO:0042802 IDA:EcoCyc F identical protein binding
GO:0030145 IDA:EcoCyc F manganese ion binding
GO:0046872 IDA:EcoCyc F metal ion binding
GO:0019288 IEA:UniProtKB-UniPathway P isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0016114 IEA:UniProtKB-HAMAP P terpenoid biosynthetic process
GO:0006744 IDA:EcoCyc P ubiquinone biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco_M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco01100 Metabolic pathways
eco00900 Terpenoid backbone biosynthesis
ko00900 Terpenoid backbone biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
NONMEVIPP-PWY methylerythritol phosphate pathway
NONMEVIPP-PWY methylerythritol phosphate pathway
PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PWY-7392 taxadiene biosynthesis (engineered)

Domain Information

InterPro Annotations

Accession Description
IPR026877 DXPR_C
IPR003821 DXP_reductoisomerase
IPR013644 DXP_reductoisomerase_C
IPR013512 DXP_reductoisomerase_N
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Protein Entry
DXR_ECOLI
UniProt ID
P45568
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=250 uM for DXP {ECO:0000269|PubMed:10787409}; Note=Measured in the presence of manganese ions.; pH dependence: Optimum pH is 7.0-8.5. {ECO:0000269|PubMed:10787409}; Temperature dependence: Optimum temperature is 40-60 degrees Celsius. {ECO:0000269|PubMed:10787409};
Catalytic Activity 2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+).;
Enzyme Regulation Inhibited by fosmidomycin.
Function Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 1/6.
Similarity Belongs to the DXR family. {ECO:0000305}.
Subunit Homodimer. {ECO:0000269|PubMed:11741911, ECO:0000269|PubMed:11872159, ECO:0000269|PubMed:12621040, ECO:0000269|PubMed:15339150, ECO:0000269|PubMed:15567415}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007574 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16128166 RefSeq NP_414715 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007574 proteins

Reference Database Accession Length Protein Name
GI:16128166 GenBank KHJ05936.1 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli]
GI:16128166 GenBank KHJ07574.1 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli]
GI:16128166 GenBank KHJ15480.1 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli]
GI:16128166 GenBank KHJ22930.1 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli]
GI:16128166 GenBank KHJ22988.1 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli]
GI:16128166 gnl IGS 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Escherichia coli ER2796]

Related Sequences to LMP007574 proteins

Reference Database Accession Length Protein Name
GI:16128166 GenBank EIQ27536.1 398 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Shigella flexneri K-315]
GI:16128166 PDB 1Q0L 406 Chain A, Crystal Structure Of Dxr In Complex With Fosmidomycin
GI:16128166 PDB 1Q0Q 406 Chain A, Crystal Structure Of Dxr In Complex With The Substrate 1- Deoxy-D-Xylulose-5-Phosphate
GI:16128166 PDB 1Q0Q 406 Chain B, Crystal Structure Of Dxr In Complex With The Substrate 1- Deoxy-D-Xylulose-5-Phosphate
GI:16128166 PDB 3R0I 410 Chain A, Ispc In Complex With An N-Methyl-Substituted Hydroxamic Acid
GI:16128166 PDB 3R0I 410 Chain B, Ispc In Complex With An N-Methyl-Substituted Hydroxamic Acid