Gene/Proteome Database (LMPD)
LMPD ID
LMP007562
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein
Gene Symbol
Synonyms
ECK0030; JW0027; lytB; yaaE
Summary
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IspH) catalyzes what is effectively the final step in the methylerythritol phosphate pathway, generating the two pathway products isopentyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). [More information is available at EcoCyc: EG11081].
Orthologs
Proteins
| 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_414570 |
| Protein GI | 16128023 |
| UniProt ID | P62623 |
| Length | 316 |
| RefSeq Status | REVIEWED |
| MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYVVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEKLSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD | |
Gene Information
Entrez Gene ID
Gene Name
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein
Gene Symbol
Species
Escherichia coli K-12
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0051538 | IEA:UniProtKB-HAMAP | F | 3 iron, 4 sulfur cluster binding |
| GO:0051745 | IEA:UniProtKB-HAMAP | F | 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity |
| GO:0042380 | IDA:EcoCyc | F | hydroxymethylbutenyl pyrophosphate reductase activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0050992 | IEA:UniProtKB-UniPathway | P | dimethylallyl diphosphate biosynthetic process |
| GO:0019288 | IEA:UniProtKB-HAMAP | P | isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
| GO:0046677 | IDA:EcoCyc | P | response to antibiotic |
| GO:0016114 | IEA:UniProtKB-HAMAP | P | terpenoid biosynthetic process |
| GO:0006744 | IDA:EcoCyc | P | ubiquinone biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| eco01110 | Biosynthesis of secondary metabolites |
| M00096 | C5 isoprenoid biosynthesis, non-mevalonate pathway |
| eco_M00096 | C5 isoprenoid biosynthesis, non-mevalonate pathway |
| eco01100 | Metabolic pathways |
| eco00900 | Terpenoid backbone biosynthesis |
| ko00900 | Terpenoid backbone biosynthesis |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| NONMEVIPP-PWY | methylerythritol phosphate pathway |
| NONMEVIPP-PWY | methylerythritol phosphate pathway |
| PWY-5121 | superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) |
| PWY-7392 | taxadiene biosynthesis (engineered) |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR003451 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase |
UniProt Annotations
Entry Information
Gene Name
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein
Protein Entry
ISPH_ECOLI
UniProt ID
Species
E. coli
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=30 uM for 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate {ECO:0000269|PubMed:15469281}; pH dependence: Optimum pH is 7.0.; |
| Catalytic Activity | Dimethylallyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. |
| Catalytic Activity | Isopentenyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. |
| Cofactor | Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}; Note=Binds 1 [3Fe-4S] cluster per subunit. {ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}; |
| Enzyme Regulation | Addition of Ca(2+), Mg(2+), Mn(2+), Ni(2+), Co(2+) or Fe(2+) decreases the activity. Addition of Zn(2+) results in complete loss of activity. {ECO:0000269|PubMed:15469281}. |
| Function | Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth. {ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}. |
| Interaction | P0A6Y8:dnaK; NbExp=1; IntAct=EBI-554022, EBI-542092; |
| Miscellaneous | PubMed:12706830 and PubMed:22137895 authors have reported the presence of a 4Fe-4S iron-sulfur cluster. It could be due to experimental conditions. |
| Pathway | Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3- methylbutenyl diphosphate: step 1/1. |
| Pathway | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 6/6. |
| Similarity | Belongs to the IspH family. {ECO:0000305}. |
| Subunit | Homodimer. {ECO:0000269|PubMed:12706830, ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007562 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16128023 | RefSeq | NP_414570 | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007562 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16128023 | GenBank | KHI77230.1 | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli] |
| GI:16128023 | GenBank | KHI95490.1 | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli] |
| GI:16128023 | GenBank | KHI99503.1 | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli] |
| GI:16128023 | GenBank | KHJ05798.1 | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli] |
| GI:16128023 | GenBank | KHJ26944.1 | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli] |
| GI:16128023 | gnl | IGS | 316 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein [Escherichia coli ER2796] |
Related Sequences to LMP007562 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16128023 | GenBank | AAN78533.1 | 331 | IspH protein [Escherichia coli CFT073] |
| GI:16128023 | GenBank | ESU79190.1 | 331 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Shigella dysenteriae WRSd3] |
| GI:16128023 | GenBank | ESU84903.1 | 331 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Shigella dysenteriae WRSd5] |
| GI:16128023 | gnl | igergo | 331 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Shigella dysenteriae 1617] |
| GI:16128023 | PDB | 3F7T | 328 | Chain B, Structure Of Active Isph Shows A Novel Fold With A [3fe-4s] Cluster In The Catalytic Centre |
| GI:16128023 | PDB | 3SZL | 328 | Chain A, Isph:ligand Mutants - Wt 70sec |