LIPID MAPS

LMPD ID

LMP007504

Gene ID

855135

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

acetolactate synthase catalytic subunit

Gene Symbol

ILV2

Synonyms

SMR1; THI1

Chromosome

XIII

EC Number

2.2.1.6

acetolactate synthase catalytic subunit
Refseq ID	NP_013826
Protein GI	6323755
UniProt ID	P07342
mRNA ID	NM_001182608
Length	687
MIRQSTLKNFAIKRCFQHIAYRNTPAMRSVALAQRFYSSSSRYYSASPLPASKRPEPAPSFNVDPLEQPAEPSKLAKKLRAEPDMDTSFVGLTGGQIFNEMMSRQNVDTVFGYPGGAILPVYDAIHNSDKFNFVLPKHEQGAGHMAEGYARASGKPGVVLVTSGPGATNVVTPMADAFADGIPMVVFTGQVPTSAIGTDAFQEADVVGISRSCTKWNVMVKSVEELPLRINEAFEIATSGRPGPVLVDLPKDVTAAILRNPIPTKTTLPSNALNQLTSRAQDEFVMQSINKAADLINLAKKPVLYVGAGILNHADGPRLLKELSDRAQIPVTTTLQGLGSFDQEDPKSLDMLGMHGCATANLAVQNADLIIAVGARFDDRVTGNISKFAPEARRAAAEGRGGIIHFEVSPKNINKVVQTQIAVEGDATTNLGKMMSKIFPVKERSEWFAQINKWKKEYPYAYMEETPGSKIKPQTVIKKLSKVANDTGRHVIVTTGVGQHQMWAAQHWTWRNPHTFITSGGLGTMGYGLPAAIGAQVAKPESLVIDIDGDASFNMTLTELSSAVQAGTPVKILILNNEEQGMVTQWQSLFYEHRYSHTHQLNPDFIKLAEAMGLKGLRVKKQEELDAKLKEFVSTKGPVLLEVEVDKKVPVLPMVAGGSGLDEFINFDPEVERQQTELRHKRTGGKH

Entrez Gene ID

855135

Gene Name

acetolactate synthase catalytic subunit

Gene Symbol

ILV2

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005948	IDA:SGD	C	acetolactate synthase complex
GO:0005739	IEA:UniProtKB-KW	C	mitochondrion
GO:0003984	IDA:SGD	F	acetolactate synthase activity
GO:0050660	IDA:SGD	F	flavin adenine dinucleotide binding
GO:0000287	IEA:InterPro	F	magnesium ion binding
GO:0030976	IEA:InterPro	F	thiamine pyrophosphate binding
GO:0009082	IDA:SGD	P	branched-chain amino acid biosynthetic process
GO:0009097	IEA:UniProtKB-UniPathway	P	isoleucine biosynthetic process
GO:0009099	IEA:UniProtKB-UniPathway	P	valine biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
ko01210	2-Oxocarboxylic acid metabolism
sce01210	2-Oxocarboxylic acid metabolism
ko01230	Biosynthesis of amino acids
sce01230	Biosynthesis of amino acids
sce01110	Biosynthesis of secondary metabolites
ko00650	Butanoate metabolism
sce00650	Butanoate metabolism
ko00660	C5-Branched dibasic acid metabolism
sce00660	C5-Branched dibasic acid metabolism
M00570	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
sce_M00570	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
sce01100	Metabolic pathways
ko00770	Pantothenate and CoA biosynthesis
sce00770	Pantothenate and CoA biosynthesis
ko00290	Valine, leucine and isoleucine biosynthesis
sce00290	Valine, leucine and isoleucine biosynthesis
M00019	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine
sce_M00019	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY3O-335	acetoin biosynthesis I
ILEUSYN-PWY	isoleucine biosynthesis
PWY3O-981	superpathway of acetoin and butanediol biosynthesis
BRANCHED-CHAIN-AA-SYN-PWY	superpathway of leucine, valine, and isoleucine biosynthesis
VALSYN-PWY	valine biosynthesis

InterPro Annotations

Accession	Description
IPR012846	Acetolactate_synth_lsu
IPR029035	DHS-like NAD/FAD-binding domain
IPR000399	TPP-binding enzyme, conserved site
IPR029061	Thiamin diphosphate-binding fold
IPR011766	Thiamine pyrophosphate enzyme, C-terminal TPP-binding
IPR012001	Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
IPR012000	Thiamine pyrophosphate enzyme, central domain

Entry Information

Gene Name

acetolactate synthase catalytic subunit

Protein Entry

ILVB_YEAST

UniProt ID

P07342

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	2 pyruvate = 2-acetolactate + CO(2).
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) ion per subunit. ;
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
Cofactor	Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000250};
Cofactor	Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
Enzyme Regulation	The regulatory subunit ILV6 stimulates enzymatic activity seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP
Enzyme Regulation	The regulatory subunit ILV6 stimulates enzymatic activity seven- to tenfold and confers sensitivity to inhibition by valine and activation by ATP. {ECO:0000269\|PubMed:10213630}.
Miscellaneous	Contains 1 molecule of FAD per monomer. The role of this cofactor is not clear considering that the reaction does not involve redox chemistry.
Miscellaneous	Present with 31900 molecules/cell in log phase SD medium
Miscellaneous	Present with 31900 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L- isoleucine from 2-oxobutanoate: step 1/4.
Pathway	Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Similarity	Belongs to the TPP enzyme family
Similarity	Belongs to the TPP enzyme family. {ECO:0000305}.
Subcellular Location	Mitochondrion.
Subunit	Homodimer. The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6
Subunit	Homodimer. The acetolactate synthase complex contains the catalytic regulatory subunit ILV2 and the regulatory small subunit ILV6. {ECO:0000269\|PubMed:12496246}.