LIPID MAPS

LMPD ID

LMP007446

Gene ID

852503

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Ldh1p

Gene Symbol

LDH1

Synonyms

-

Chromosome

II

EC Number

3.1.1.-

Ldh1p
Refseq ID	NP_009763
Protein GI	330443463
UniProt ID	P38139
mRNA ID	NM_001178552
Length	375
MNMAERAEATKSWSCEPLSGKTLEEIVQNAENAADLVAYIRKPEVDLDFRLKFIAEHEEFFNVQLSDRNSRIRTCHNLSDKGIRGDTVFVFVPGLAGNLEQFEPLLELVDSDQKAFLTLDLPGFGHSSEWSDYPMLKVVELIFVLVCDVLRKWSTAVPNNDNVNPFNGHKIVLVGHSMGCFLACHLYEQHMADTKAVQTLVLLTPPKAHIEQLSKDKHIIQWALYGVFKLPWLFDVYRNKFDQVKGLQSSGIKQYFYQQGDDVKLKYRKFWQFKNNISNKSRTIIGYLLGWETVDWVKFNGVLTQTDMKQKIIIFGAEKDPIAPIENLEFYKQTINKECLRKVIILPDCSHNLCFDRPELVCENFQREVIDNSKL

Entrez Gene ID

852503

Gene Name

Ldh1p

Gene Symbol

LDH1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005811	IDA:SGD	C	lipid particle
GO:0016788	IDA:SGD	F	hydrolase activity, acting on ester bonds
GO:0004806	IDA:SGD	F	triglyceride lipase activity
GO:0016042	IEA:UniProtKB-KW	P	lipid catabolic process
GO:0055088	IMP:SGD	P	lipid homeostasis

InterPro Annotations

Accession	Description
IPR029058	Alpha/Beta hydrolase fold
IPR000073	Alpha/beta hydrolase fold-1

Entry Information

Gene Name

Ldh1p

Protein Entry

LDH1_YEAST

UniProt ID

P38139

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=0.77 uM for p-nitrophenyl butyrate (PNB) {ECO:0000269\|PubMed:21478434}; KM=3.3 mM for 1,2-dioleoyl-3-(pyren-1-yl)-decanoyl-rac-glycerol (DPG) {ECO:0000269\|PubMed:21478434}; Vmax=0.041 umol/min/mg enzyme for esterase activity using PNB as a substrate {ECO:0000269\|PubMed:21478434}; Vmax=1 umol/min/mg enzyme for triacylglycerol lipase activity using DPG as a substrate {ECO:0000269\|PubMed:21478434};
Biophysicochemical Properties	Kinetic parameters: KM=0.77 uM for p-nitrophenyl butyrate (PNB) ; KM=3.3 mM for 1,2-dioleoyl-3-(pyren-1-yl)-decanoyl-rac-glycerol (DPG) ; Vmax=0.041 umol/min/mg enzyme for esterase activity using PNB as a substrate ; Vmax=1 umol/min/mg enzyme for triacylglycerol lipase activity using DPG as a substrate ;
Catalytic Activity	Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.
Disruption Phenotype	Leads to the appearance of giant lipid droplets and an excessive accumulation of non-polar lipids and phospholipids upon growth on medium containing oleic acid as a sole carbon source
Disruption Phenotype	Leads to the appearance of giant lipid droplets and an excessive accumulation of non-polar lipids and phospholipids upon growth on medium containing oleic acid as a sole carbon source. {ECO:0000269\|PubMed:21478434}.
Function	Serine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis. Exhibits both esterase and triacylglycerol lipase activity
Function	Serine hydrolase required for the maintenance of steady state level of non-polar and polar lipids of lipid droplets and thus plays a role in maintaining the lipids homeostasis. Exhibits both esterase and triacylglycerol lipase activity. {ECO:0000269\|PubMed:21478434}.
Similarity	Belongs to the AB hydrolase superfamily. Lipase family
Similarity	Belongs to the AB hydrolase superfamily. Lipase family. {ECO:0000305}.
Subcellular Location	Lipid droplet .
Subcellular Location	Lipid droplet {ECO:0000269\|PubMed:21478430}.