LIPID MAPS

LMPD ID

LMP007371

Gene ID

852394

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Vps15p

Gene Symbol

VPS15

Synonyms

GRD8; VAC4; VPL19; VPS40; VPT15

Chromosome

II

EC Number

2.7.11.1

Vps15p
Refseq ID	NP_009655
Protein GI	330443447
UniProt ID	P22219
mRNA ID	NM_001178445
Length	1454
MGAQLSLVVQASPSIAIFSYIDVLEEVHYVSQLNSSRFLKTCKALDPNGEIVIKVFIKPKDQYSLRPFLQRIRAQSFKLGQLPHVLNYSKLIETNRAGYMIRQHLKNNLYDRLSLRPYLQDIELKFIAFQLLNALKDIHNLNIVHGDIKTENILVTSWNWCILTDFAAFIKPVYLPEDNPGEFLFYFDTSKRRTCYLAPERFNSKLYQDGKSNNGRLTKEMDIFSLGCVIAEIFAEGRPIFNLSQLFKYKSNSYDVNREFLMEEMNSTDLRNLVLDMIQLDPSKRLSCDELLNKYRGIFFPDYFYTFIYDYFRNLVTMTTSTPISDNTCTNSTLEDNVKLLDETTEKIYRDFSQICHCLDFPLIKDGGEIGSDPPILESYKIEIEISRFLNTNLYFPQNYHLVLQQFTKVSEKIKSVKEECALLFISYLSHSIRSIVSTATKLKNLELLAVFAQFVSDENKIDRVVPYFVCCFEDSDQDVQALSLLTLIQVLTSVRKLNQLNENIFVDYLLPRLKRLLISNRQNTNYLRIVFANCLSDLAIIINRFQEFTFAQHCNDNSMDNNTEIMESSTKYSAKLIQSVEDLTVSFLTDNDTYVKMALLQNILPLCKFFGRERTNDIILSHLITYLNDKDPALRVSLIQTISGISILLGTVTLEQYILPLLIQTITDSEELVVISVLQSLKSLFKTGLIRKKYYIDISKTTSPLLLHPNNWIRQFTLMIIIEIINKLSKAEVYCILYPIIRPFFEFDVEFNFKSMISCCKQPVSRSVYNLLCSWSVRASKSLFWKKIITNHVDSFGNNRIEFITKNYSSKNYGFNKRDTKSSSSLKGIKTSSTVYSHDNKEIPLTAEDRNWIDKFHIIGLTEKDIWKIVALRGYVIRTARVMAANPDFPYNNSNYRPLVQNSPPNLNLTNIMPRNIFFDVEFAEESTSEGQDSNLENQQIYKYDESEKDSNKLNINGSKQLSTVMDINGSLIFKNKSIATTTSNLKNVFVQLEPTSYHMHSPNHGLKDNANVKPERKVVVSNSYEGDVESIEKFLSTFKILPPLRDYKEFGPIQEIVRSPNMGNLRGKLIATLMENEPNSITSSAVSPGETPYLITGSDQGVIKIWNLKEIIVGEVYSSSLTYDCSSTVTQITMIPNFDAFAVSSKDGQIIVLKVNHYQQESEVKFLNCECIRKINLKNFGKNEYAVRMRAFVNEEKSLLVALTNLSRVIIFDIRTLERLQIIENSPRHGAVSSICIDEECCVLILGTTRGIIDIWDIRFNVLIRSWSFGDHAPITHVEVCQFYGKNSVIVVGGSSKTFLTIWNFVKGHCQYAFINSDEQPSMEHFLPIEKGLEELNFCGIRSLNALSTISVSNDKILLTDEATSSIVMFSLNELSSSKAVISPSRFSDVFIPTQVTANLTMLLRKMKRTSTHSVDDSLYHHDIINSISTCEVDETPLLVACDNSGLIGIFQ

Entrez Gene ID

852394

Gene Name

Vps15p

Gene Symbol

VPS15

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005794	IEA:UniProtKB-KW	C	Golgi apparatus
GO:0005768	IEA:UniProtKB-KW	C	endosome
GO:0071561	IDA:SGD	C	nucleus-vacuole junction
GO:0034271	IDA:SGD	C	phosphatidylinositol 3-kinase complex, class III, type I
GO:0034272	IDA:SGD	C	phosphatidylinositol 3-kinase complex, class III, type II
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0004674	IDA:SGD	F	protein serine/threonine kinase activity
GO:0043130	IDA:SGD	F	ubiquitin binding
GO:0048017	IMP:SGD	P	inositol lipid-mediated signaling
GO:0045324	IMP:SGD	P	late endosome to vacuole transport
GO:0016236	IMP:SGD	P	macroautophagy
GO:0030242	IMP:SGD	P	peroxisome degradation
GO:0032968	IMP:SGD	P	positive regulation of transcription elongation from RNA polymerase II promoter
GO:0006468	IDA:SGD	P	protein phosphorylation
GO:0045053	IMP:SGD	P	protein retention in Golgi apparatus
GO:0006623	IMP:SGD	P	protein targeting to vacuole
GO:0000011	IMP:SGD	P	vacuole inheritance

KEGG Pathway Links

KEGG Pathway ID	Description
ko04140	Regulation of autophagy
sce04140	Regulation of autophagy

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618611	PI Metabolism
5618126	Phospholipid metabolism
5618614	Synthesis of PIPs at the Golgi membrane
5618615	Synthesis of PIPs at the early endosome membrane
5618612	Synthesis of PIPs at the late endosome membrane

InterPro Annotations

Accession	Description
IPR011989	Armadillo-like helical
IPR016024	Armadillo-type fold
IPR021133	HEAT, type 2
IPR000719	Protein kinase domain
IPR011009	Protein kinase-like domain
IPR008271	Serine/threonine-protein kinase, active site
IPR017986	WD40-repeat-containing domain
IPR015943	WD40/YVTN repeat-like-containing domain
IPR001680	WD40_repeat

Entry Information

Gene Name

Vps15p

Protein Entry

VPS15_YEAST

UniProt ID

P22219

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	ATP + a protein = ADP + a phosphoprotein.
Domain	Truncation of 30 residues from the C-terminus results in a temperature-conditional defect in protein sorting.
Function	Serine/threonine-protein kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Is also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). By regulating VPS34 kinase activity, VPS15 appears to be essential for the efficient delivery of soluble hydrolases to the yeast vacuole. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:12244127, ECO:0000269\|PubMed:1756716, ECO:0000269\|PubMed:1988155, ECO:0000269\|PubMed:3062374, ECO:0000269\|PubMed:7989323, ECO:0000269\|PubMed:8387919, ECO:0000269\|PubMed:8509446, ECO:0000269\|PubMed:8649377}.
Interaction	P08539:GPA1; NbExp=2; IntAct=EBI-20347, EBI-7376;
Miscellaneous	Present with 279 molecules/cell in log phase SD medium
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Ptm	Autophosphorylated.
Similarity	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family
Similarity	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. {ECO:0000255\|PROSITE-ProRule:PRU00159}.
Similarity	Contains 1 protein kinase domain
Similarity	Contains 1 protein kinase domain. {ECO:0000255\|PROSITE-ProRule:PRU00159}.
Similarity	Contains 4 HEAT repeats. {ECO:0000255\|PROSITE- ProRule:PRU00103}.
Similarity	Contains 6 WD repeats. {ECO:0000255\|PROSITE- ProRule:PRU00221}.
Subcellular Location	Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Endosome membrane; Lipid-anchor.
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. {ECO:0000269\|PubMed:24165940}.