Gene/Proteome Database (LMPD)

LMPD ID
LMP007332
Gene ID
851878
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
Gene Symbol
DPP1
Synonyms
ZRG1
Alternate Names
bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
Chromosome
IV
EC Number
3.1.3.81

Proteins

bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
Refseq ID NP_010570
Protein GI 6320490
UniProt ID Q05521
mRNA ID NM_001180592
Length 289
RefSeq Status PROVISIONAL
MNRVSFIKTPFNIGAKWRLEDVFLLIIMILLNYPVYYQQPFERQFYINDLTISHPYATTERVNNNMLFVYSFVVPSLTILIIGSILADRRHLIFILYTSLLGLSLAWFSTSFFTNFIKNWIGRLRPDFLDRCQPVEGLPLDTLFTAKDVCTTKNHERLLDGFRTTPSGHSSESFAGLGYLYFWLCGQLLTESPLMPLWRKMVAFLPLLGAALIALSRTQDYRHHFVDVILGSMLGYIMAHFFYRRIFPPIDDPLPFKPLMDDSDVTLEEAVTHQRIPDEELHPLSDEGM

Gene Information

Entrez Gene ID
851878
Gene Name
bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
Gene Symbol
DPP1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000329 IDA:SGD C fungal-type vacuole membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0000810 IDA:SGD F diacylglycerol diphosphate phosphatase activity
GO:0008195 IDA:SGD F phosphatidate phosphatase activity
GO:0016311 IDA:GOC P dephosphorylation
GO:0006644 IMP:SGD P phospholipid metabolic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
TRIGLSYN-PWY triacylglycerol biosynthesis
TRIGLSYN-PWY triacylglycerol biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5618411 Fcgamma receptor (FCGR) dependent phagocytosis
5618410 Role of phospholipids in phagocytosis

Domain Information

InterPro Annotations

Accession Description
IPR000326 Phosphatidic acid phosphatase type 2/haloperoxidase

UniProt Annotations

Entry Information

Gene Name
bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase
Protein Entry
DPP1_YEAST
UniProt ID
Q05521
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: Vmax=167 umol/min/mg enzyme with lysophosphatidic acid as substrate {ECO:0000269|PubMed:8940025}; pH dependence: Optimum pH is 6.0-8.5. {ECO:0000269|PubMed:8940025};
Catalytic Activity 1,2-diacyl-sn-glycerol 3-diphosphate + H(2)O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate. {ECO:0000269|PubMed:8567632}.
Catalytic Activity A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate. {ECO:0000269|PubMed:8567632}.
Domain The phosphatase sequence motif I (including Arg-125) and II (including His-169) are part of the cytoplasmic loop 2 and phosphatase sequence motif III (including His-223) is part of the cytoplasmic loop 3.
Enzyme Regulation Inhibited by sodium fluoride (NaF) and pyrophosphate. Strongly inhibited by manganese ion and, to a lower extent, by magnesium and calcium ions. Also inhibited by Cu(2+) ion. In an indirect manner, it is also inhibited by the zinc ion which is able to form a complex with DGPP and prevent the enzyme from removing the phosphate from the substrate. Not inhibited by N-ethylmaleimide. {ECO:0000269|PubMed:11139591, ECO:0000269|PubMed:8567632}.
Function Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Together with LPP1, regulates intracellular DGPP and PA levels, which are phospholipid molecules believed to play a signaling role in stress response. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. Substrate preference is DGPP > LPA > PA. Activity is independent of a divalent cation ion and insensitive to inhibition by N-ethylmaleimide. {ECO:0000269|PubMed:11139591, ECO:0000269|PubMed:8567632}.
Induction Induced by the transcription factor ZAP1 during zinc depletion and negatively regulated by the transcription factor GIS1 predominantly during nutrient limitation. Induced by inositol supplementation. {ECO:0000269|PubMed:11139591, ECO:0000269|PubMed:12799368, ECO:0000269|PubMed:14642771}.
Miscellaneous Present with 3038 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity Belongs to the PA-phosphatase related phosphoesterase family. {ECO:0000305}.
Subcellular Location Vacuole membrane {ECO:0000269|PubMed:11139591, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:8567632}; Multi-pass membrane protein {ECO:0000269|PubMed:11139591, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:8567632}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007332 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6320490 RefSeq NP_010570 289 bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase

Identical Sequences to LMP007332 proteins

Reference Database Accession Length Protein Name
GI:6320490 DBBJ GAA22505.1 289 K7_Dpp1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6320490 GenBank EGA79444.1 289 Dpp1p [Saccharomyces cerevisiae Vin13]
GI:6320490 GenBank EIW11488.1 289 Dpp1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6320490 GenBank EWG96584.1 289 Dpp1p [Saccharomyces cerevisiae R103]
GI:6320490 GenBank EWH18993.1 289 Dpp1p [Saccharomyces cerevisiae P283]
GI:6320490 gnl McCuskerlabDuke 289 Dpp1p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP007332 proteins

Reference Database Accession Length Protein Name
GI:6320490 GenBank EGA59145.1 289 Dpp1p [Saccharomyces cerevisiae FostersB]
GI:6320490 GenBank EGA83399.1 289 Dpp1p [Saccharomyces cerevisiae Lalvin QA23]
GI:6320490 GenBank EGA87429.1 289 Dpp1p [Saccharomyces cerevisiae VL3]
GI:6320490 GenBank EHN03014.1 291 Dpp1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6320490 GenBank EHN07856.1 289 Dpp1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]
GI:6320490 GenBank EWG87301.1 289 Dpp1p [Saccharomyces cerevisiae R008]