LIPID MAPS

LMPD ID

LMP007267

Gene ID

851102

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Vac14p

Gene Symbol

VAC14

Synonyms

-

Chromosome

XII

Vac14p
Refseq ID	NP_013490
Protein GI	398366065
UniProt ID	Q06708
mRNA ID	NM_001182275
Length	880
MEKSIAKGLSDKLYEKRKAAALELEKLVKQCVLEGDYDRIDKIIDELCRDYAYALHQPMARNAGLMGLAATAIALGINDVGRYLRNILPPVLACFGDQNDQVRFYACESLYNIAKIAKGEILVYFNEIFDVLCKISADTENSVRGAAELLDRLIKDIVAERASNYISIVNNGSHGLLPAIKTDPISGDVYQEEYEQDNQLAFSLPKFIPLLTERIYAINPDTRVFLVDWLKVLLNTPGLELISYLPSFLGGLFTFLGDSHKDVRTVTHTLMDSLLHEVDRISKLQTEIKMKRLERLKMLEDKYNNSSTPTKKADGALIAEKKKTLMTALGGLSKPLSMETDDTKLSNTNETDDERHLTSQEQLLDSEATSQEPLRDGEEYIPGQDINLNFPEVITVLVNNLASSEAEIQLIALHWIQVILSISPNVFIPFLSKILSVLLKLLSDSDPHITEIAQLVNGQLLSLCSSYVGKETDGKIAYGPIVNSLTLQFFDSRIDAKIACLDWLILIYHKAPNQILKHNDSMFLTLLKSLSNRDSVLIEKALSLLQSLCSDSNDNYLRQFLQDLLTLFKRDTKLVKTRANFIMRQISSRLSPERVYKVISSILDNYNDTTFVKMMIQILSTNLITSPEMSSLRNKLRTCEDGMFFNSLFKSWCPNPVSVISLCFVAENYELAYTVLQTYANYELKLNDLVQLDILIQLFESPVFTRMRLQLLEQQKHPFLHKCLFGILMIIPQSKAFETLNRRLNSLNIWTSQSYVMNNYIRQRENSNFCDSNSDISQRSVSQSKLHFQELINHFKAVSEEDEYSSDMIRLDHGANNKSLLLGSFLDGIDEDKQEIVTPISPMNEAINEEMESPNDNSSVILKDSGSLPFNRNVSDKLKK

Entrez Gene ID

851102

Gene Name

Vac14p

Gene Symbol

VAC14

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0070772	IDA:SGD	C	PAS complex
GO:0000306	IDA:SGD	C	extrinsic component of vacuolar membrane
GO:0000329	IDA:SGD	C	fungal-type vacuole membrane
GO:0042802	IPI:IntAct	F	identical protein binding
GO:0030674	IDA:SGD	F	protein binding, bridging
GO:0006661	IMP:SGD	P	phosphatidylinositol biosynthetic process
GO:0033674	IMP:SGD	P	positive regulation of kinase activity
GO:0015031	IEA:UniProtKB-KW	P	protein transport
GO:0043550	IEA:InterPro	P	regulation of lipid kinase activity

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618611	PI Metabolism
5618126	Phospholipid metabolism
5618614	Synthesis of PIPs at the Golgi membrane
5618615	Synthesis of PIPs at the early endosome membrane
5618612	Synthesis of PIPs at the late endosome membrane

InterPro Annotations

Accession	Description
IPR011989	Armadillo-like helical
IPR016024	Armadillo-type fold
IPR000357	HEAT
IPR021133	HEAT, type 2
IPR021841	Vacuolar protein 14 C-terminal Fig4-binding domain
IPR026825	Vacuole morphology and inheritance protein 14

Entry Information

Gene Name

Vac14p

Protein Entry

VAC14_YEAST

UniProt ID

Q06708

Species

Yeast (S288c)

Comments

Comment Type	Description
Domain	The N-terminal domain mediates interaction with FAB1 and VAC7 while the C-terminal domain mediates interaction with FIG4
Domain	The N-terminal domain mediates interaction with FAB1 and VAC7 while the C-terminal domain mediates interaction with FIG4. {ECO:0000269\|PubMed:19037259}.
Function	The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Regulates the synthesis of PtdIns(3,5)P2 by positive activation of FAB1 and by controlling FIG4 localization. Required for FIG4-mediated turnover of PtdIns(3,5)P2 after hyperosmotic shock. Essential for the control of trafficking of some proteins to the vacuole lumen via the multivesicular body (MVB), and for maintenance of vacuole size and acidity. {ECO:0000269\|PubMed:11889142, ECO:0000269\|PubMed:12062051, ECO:0000269\|PubMed:14528018, ECO:0000269\|PubMed:16492811, ECO:0000269\|PubMed:19037259}.
Interaction	Self; NbExp=4; IntAct=EBI-27189, EBI-27189; P43601:ATG18; NbExp=5; IntAct=EBI-27189, EBI-22968; P34756:FAB1; NbExp=9; IntAct=EBI-27189, EBI-6754; P42837:FIG4; NbExp=7; IntAct=EBI-27189, EBI-28407; P53950:VAC7; NbExp=4; IntAct=EBI-27189, EBI-28714;
Miscellaneous	Present with 12388 molecules/cell in log phase SD medium
Miscellaneous	Present with 12388 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the VAC14 family
Similarity	Belongs to the VAC14 family. {ECO:0000305}.
Similarity	Contains 5 HEAT repeats. {ECO:0000255\|PROSITE- ProRule:PRU00103}.
Subcellular Location	Vacuole membrane {ECO:0000269\|PubMed:11889142, ECO:0000269\|PubMed:14528018, ECO:0000269\|PubMed:14562095, ECO:0000269\|PubMed:18653468, ECO:0000269\|PubMed:19037259}; Peripheral membrane protein {ECO:0000269\|PubMed:11889142, ECO:0000269\|PubMed:14528018, ECO:0000269\|PubMed:14562095, ECO:0000269\|PubMed:18653468, ECO:0000269\|PubMed:19037259}. Note=Limiting membrane of the vacuole. Localization requires FAB1 and FIG4.
Subunit	Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold
Subunit	Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. {ECO:0000269\|PubMed:18653468, ECO:0000269\|PubMed:19037259}.