Gene/Proteome Database (LMPD)

LMPD ID
LMP007248
Gene ID
853768
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
Yju3p
Gene Symbol
YJU3
Synonyms
-
Alternate Names
Yju3p
Chromosome
XI
EC Number
3.1.1.23

Proteins

Yju3p
Refseq ID NP_012829
Protein GI 6322756
UniProt ID P28321
mRNA ID NM_001179660
Length 313
RefSeq Status PROVISIONAL
MAPYPYKVQTTVPELQYENFDGAKFGYMFWPVQNGTNEVRGRVLLIHGFGEYTKIQFRLMDHLSLNGYESFTFDQRGAGVTSPGRSKGVTDEYHVFNDLEHFVEKNLSECKAKGIPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLIILHPHTMYNKPTQIIAPLLAKFLPRVRIDTGLDLKGITSDKAYRAFLGSDPMSVPLYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHGQDDTINDPKGSEKFIQDCPSADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWLDKHTTTEAKP

Gene Information

Entrez Gene ID
853768
Gene Name
Yju3p
Gene Symbol
YJU3
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0005811 IDA:SGD C lipid particle
GO:0016020 IDA:SGD C membrane
GO:0005741 IEA:UniProtKB-KW C mitochondrial outer membrane
GO:0047372 IMP:SGD F acylglycerol lipase activity
GO:0019433 IEA:UniProtKB-UniPathway P triglyceride catabolic process
GO:0006641 IMP:SGD P triglyceride metabolic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-7420 monoacylglycerol metabolism
LIPAS-PWY triacylglycerol degradation

REACTOME Pathway Links

REACTOME Pathway ID Description
5618409 GPCR downstream signaling
5618266 Platelet activation, signaling and aggregation

Domain Information

InterPro Annotations

Accession Description
IPR029058 Alpha/Beta hydrolase fold
IPR000073 Alpha/beta hydrolase fold-1

UniProt Annotations

Entry Information

Gene Name
Yju3p
Protein Entry
MGLL_YEAST
UniProt ID
P28321
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=260 uM for rac-1(3)-oleoylglycerol {ECO:0000269|PubMed:20554061}; Vmax=10.3 mmol/h/mg enzyme {ECO:0000269|PubMed:20554061}; pH dependence: Active from pH 4.5 to 8. {ECO:0000269|PubMed:20554061};
Catalytic Activity Hydrolyzes glycerol monoesters of long-chain fatty acids.
Function Converts monoacylglycerides (MAG) to free fatty acids and glycerol. Required for efficient degradation of MAG, short- lived intermediates of glycerolipid metabolism which may also function as lipid signaling molecules. Controls inactivation of the signaling lipid N-palmitoylethanolamine (PEA). {ECO:0000269|PubMed:19529773, ECO:0000269|PubMed:20554061}.
Miscellaneous Present with 2140 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Glycerolipid metabolism; triacylglycerol degradation.
Similarity Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. {ECO:0000305}.
Subcellular Location Cytoplasm. Endoplasmic reticulum. Lipid droplet. Mitochondrion outer membrane. Note=Although the protein was identified in the cytoplasm, several membrane systems and lipid droplets, MGL activity was only measured in membrane fractions and lipid droplets.

Identical and Related Proteins

Unique RefSeq proteins for LMP007248 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6322756 RefSeq NP_012829 313 Yju3p

Identical Sequences to LMP007248 proteins

Reference Database Accession Length Protein Name
GI:6322756 DBBJ GAA24632.1 313 K7_Yju3p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6322756 EMBL CBK51692.1 313 unnamed protein product [Saccharomyces cerevisiae]
GI:6322756 GenBank ABZ48307.1 313 Sequence 22245 from patent US 7314974
GI:6322756 GenBank EIW09138.1 313 Yju3p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:6322756 SwissProt P28321.2 313 RecName: Full=Monoglyceride lipase; Short=MGL; AltName: Full=Monoacylglycerol hydrolase; Short=MAG hydrolase; Short=MGH; AltName: Full=Monoacylglycerol lipase; Short=MAG lipase; Short=MAGL; AltName: Full=Serine hydrolase YJU3 [Saccharomyces cerevisiae S288c]
GI:6322756 Third Party Genbank DAA09064.1 313 TPA: Yju3p [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007248 proteins

Reference Database Accession Length Protein Name
GI:6322756 EMBL CBD18346.1 313 unnamed protein product [Saccharomyces cerevisiae]
GI:6322756 EMBL CBC65080.1 313 unnamed protein product [Saccharomyces cerevisiae]
GI:6322756 GenBank EGA61615.1 313 Yju3p [Saccharomyces cerevisiae FostersO]
GI:6322756 GenBank EWG90065.1 313 Yju3p [Saccharomyces cerevisiae P301]
GI:6322756 GenBank EWG94912.1 313 Yju3p [Saccharomyces cerevisiae R103]
GI:6322756 GenBank EWH17550.1 313 Yju3p [Saccharomyces cerevisiae P283]