LIPID MAPS

LMPD ID

LMP007206

Gene ID

856303

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

AAA family ATPase VPS4

Gene Symbol

VPS4

Synonyms

CSC1; DID6; END13; GRD13; VPL4; VPT10

Chromosome

XVI

AAA family ATPase VPS4
Refseq ID	NP_015499
Protein GI	6325431
UniProt ID	P52917
mRNA ID	NM_001184270
Length	437
MSTGDFLTKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKFTEYLNRAEQLKKHLESEEANAAKKSPSAGSGSNGGNKKISQEEGEDNGGEDNKKLRGALSSAILSEKPNVKWEDVAGLEGAKEALKEAVILPVKFPHLFKGNRKPTSGILLYGPPGTGKSYLAKAVATEANSTFFSVSSSDLVSKWMGESEKLVKQLFAMARENKPSIIFIDEVDALTGTRGEGESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDSAIRRRFERRIYIPLPDLAARTTMFEINVGDTPCVLTKEDYRTLGAMTEGYSGSDIAVVVKDALMQPIRKIQSATHFKDVSTEDDETRKLTPCSPGDDGAIEMSWTDIEADELKEPDLTIKDFLKAIKSTRPTVNEDDLLKQEQFTRDFGQEGN

Entrez Gene ID

856303

Gene Name

AAA family ATPase VPS4

Gene Symbol

VPS4

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005768	IDA:SGD	C	endosome
GO:0010008	IMP:GOC	C	endosome membrane
GO:0016020	IDA:SGD	C	membrane
GO:0005524	IDA:SGD	F	ATP binding
GO:0016887	IDA:SGD	F	ATPase activity
GO:0042803	IDA:SGD	F	protein homodimerization activity
GO:0006200	IDA:GOC	P	ATP catabolic process
GO:0070676	IMP:SGD	P	intralumenal vesicle formation
GO:0045324	IMP:SGD	P	late endosome to vacuole transport
GO:0032511	IMP:SGD	P	late endosome to vacuole transport via multivesicular body sorting pathway
GO:0051260	IDA:SGD	P	protein homooligomerization
GO:0045053	IMP:SGD	P	protein retention in Golgi apparatus
GO:0015031	IEA:UniProtKB-KW	P	protein transport
GO:0016125	IMP:SGD	P	sterol metabolic process

KEGG Pathway Links

KEGG Pathway ID	Description
ko04144	Endocytosis
sce04144	Endocytosis

InterPro Annotations

Accession	Description
IPR003593	AAA+ ATPase domain
IPR003960	ATPase, AAA-type, conserved site
IPR003959	ATPase, AAA-type, core
IPR007330	MIT
IPR027417	P-loop containing nucleoside triphosphate hydrolase
IPR015415	Vps4_C

Entry Information

Gene Name

AAA family ATPase VPS4

Protein Entry

VPS4_YEAST

UniProt ID

P52917

Species

Yeast (S288c)

Comments

Comment Type	Description
Function	Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex. {ECO:0000269\|PubMed:11329380, ECO:0000269\|PubMed:9155008, ECO:0000269\|PubMed:9606181}.
Interaction	P39929:SNF7; NbExp=4; IntAct=EBI-20475, EBI-17554; Q04272:VPS20; NbExp=6; IntAct=EBI-20475, EBI-28157; Q06263:VTA1; NbExp=7; IntAct=EBI-20475, EBI-37098;
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium
Miscellaneous	Present with 5350 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the AAA ATPase family
Similarity	Belongs to the AAA ATPase family. {ECO:0000305}.
Similarity	Contains 1 MIT domain
Similarity	Contains 1 MIT domain. {ECO:0000305}.
Subcellular Location	Endosome membrane ; Peripheral membrane protein .
Subcellular Location	Endosome membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Subunit	Monomer or homodimer (in nucleotide-free form). Decamer, dodecamer or tetradecamer of two stacked respective homooligomeric rings (when bound to ATP); the dodecameric form seems to be predominant. Interacts with VPS20. Interacts with VTA1; the interaction requires the dimeric structure of VTA1; 6 homodimers of VTA1 appear to associate with the dodecameric VSP4 complex. Interacts with DID2. Interacts with DID4. Interacts with IST1; IST1 competes with VTA1 for binding with VPS4. {ECO:0000269\|PubMed:12953057, ECO:0000269\|PubMed:15086794, ECO:0000269\|PubMed:16505166, ECO:0000269\|PubMed:16601096, ECO:0000269\|PubMed:17130288, ECO:0000269\|PubMed:17928861, ECO:0000269\|PubMed:17949747, ECO:0000269\|PubMed:18032584, ECO:0000269\|PubMed:18280501, ECO:0000269\|PubMed:9606181}.