LIPID MAPS

LMPD ID

LMP007189

Gene ID

851479

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

branched-chain-2-oxoacid decarboxylase THI3

Gene Symbol

THI3

Synonyms

KID1

Chromosome

IV

EC Number

4.1.1.-

branched-chain-2-oxoacid decarboxylase THI3
Refseq ID	NP_010203
Protein GI	6320123
UniProt ID	Q07471
mRNA ID	NM_001180139
Length	609
MNSSYTQRYALPKCIAISDYLFHRLNQLNIHTIFGLSGEFSMPLLDKLYNIPNLRWAGNSNELNAAYAADGYSRLKGLGCLITTFGVGELSAINGVAGSYAEHVGILHIVGMPPTSAQTKQLLLHHTLGNGDFTVFHRIASDVACYTTLIIDSELCADEVDKCIKKAWIEQRPVYMGMPVNQVNLPIESARLNTPLDLQLHKNDPDVEKEVISRILSFIYKSQNPAIIVDACTSRQNLIEETKELCNRLKFPVFVTPMGKGTVNETDPQFGGVFTGSISAPEVREVVDFADFIIVIGCMLSEFSTSTFHFQYKTKNCALLYSTSVKLKNATYPDLSIKLLLQKILANLDESKLSYQPSEQPSMMVPRPYPAGNVLLRQEWVWNEISHWFQPGDIIITETGASAFGVNQTRFPVNTLGISQALWGSVGYTMGACLGAEFAVQEINKDKFPATKHRVILFMGDGAFQLTVQELSTIVKWGLTPYIFVMNNQGYSVDRFLHHRSDASYYDIQPWNYLGLLRVFGCTNYETKKIITVGEFRSMISDPNFATNDKIRMIEIMLPPRDVPQALLDRWVVEKEQSKQVQEENENSSAVNTPTPEFQPLLKKNQVGY

Entrez Gene ID

851479

Gene Name

branched-chain-2-oxoacid decarboxylase THI3

Gene Symbol

THI3

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005634	IDA:SGD	C	nucleus
GO:0001102	IPI:SGD	F	RNA polymerase II activating transcription factor binding
GO:0016829	IEA:UniProtKB-KW	F	lyase activity
GO:0000287	IEA:InterPro	F	magnesium ion binding
GO:0030976	IEA:InterPro	F	thiamine pyrophosphate binding
GO:0000955	IEA:UniProtKB-UniPathway	P	amino acid catabolic process via Ehrlich pathway
GO:0009083	IEA:UniProtKB-KW	P	branched-chain amino acid catabolic process
GO:0090180	IMP:SGD	P	positive regulation of thiamine biosynthetic process
GO:0045944	IMP:SGD	P	positive regulation of transcription from RNA polymerase II promoter

KEGG Pathway Links

KEGG Pathway ID	Description
sce01110	Biosynthesis of secondary metabolites
ko00010	Glycolysis / Gluconeogenesis
sce00010	Glycolysis / Gluconeogenesis
sce01100	Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-6871	3-methylbutanol biosynthesis
PWY3O-4109	isoleucine degradation
PWY-5078	isoleucine degradation II
PWY-5078	isoleucine degradation II
PWY3O-4112	leucine degradation
PWY-5076	leucine degradation III
PWY-5076	leucine degradation III

InterPro Annotations

Accession	Description
IPR029035	DHS-like NAD/FAD-binding domain
IPR029061	Thiamin diphosphate-binding fold
IPR012110	Thiamine pyrophosphate (TPP)-dependent enzyme
IPR011766	Thiamine pyrophosphate enzyme, C-terminal TPP-binding
IPR012001	Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain
IPR012000	Thiamine pyrophosphate enzyme, central domain

Entry Information

Gene Name

branched-chain-2-oxoacid decarboxylase THI3

Protein Entry

THI3_YEAST

UniProt ID

Q07471

Species

Yeast (S288c)

Comments

Comment Type	Description
Biotechnology	Fusel oils are important flavor and aroma compounds in yeast-fermented products contributing to the quality of beverages and food. In low concentration they are generally desirable, whereas high concentrations may spoil the product. By adjusting growth conditions and substrate their production is sought to be influenced.
Catalytic Activity	A 2-oxo acid = an aldehyde + CO(2).
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ; Note=Binds 1 Mg(2+) per subunit. ;
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 1 Mg(2+) per subunit. {ECO:0000250};
Cofactor	Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000250}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000250};
Cofactor	Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence= ; Note=Binds 1 thiamine pyrophosphate per subunit. ;
Function	One of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) involved in amino acid catabolism. The enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids (alpha-keto-acids). In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids leucine and isoleucine, whereas valine, aromatic amino acids, and pyruvate are no substrates. In analogy to the pyruvate decarboxylases the enzyme may in a side-reaction catalyze condensation (or carboligation) reactions leading to the formation of 2-hydroxy ketone, collectively called acyloins. The enzyme is also positively regulating the thiamine metabolism by a molecular mechanism that may involve thiamine concentration sensing and signal transmission. {ECO:0000269\|PubMed:10753893, ECO:0000269\|PubMed:12499363, ECO:0000269\|PubMed:12902239, ECO:0000269\|PubMed:1624458, ECO:0000269\|PubMed:9341119, ECO:0000269\|PubMed:9748245}.
Interaction	P32896:PDC2; NbExp=3; IntAct=EBI-19209, EBI-13004;
Miscellaneous	Present with 2140 molecules/cell in log phase SD medium
Miscellaneous	Present with 2140 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Amino-acid degradation; Ehrlich pathway.
Similarity	Belongs to the TPP enzyme family
Similarity	Belongs to the TPP enzyme family. {ECO:0000305}.
Subcellular Location	Nucleus .
Subcellular Location	Nucleus {ECO:0000269\|PubMed:14562095}.