Gene/Proteome Database (LMPD)

LMPD ID
LMP007158
Gene ID
853632
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
phosphatidylinositol kinase-related protein kinase TOR2
Gene Symbol
TOR2
Synonyms
DRR2
Alternate Names
phosphatidylinositol kinase-related protein kinase TOR2
Chromosome
XI
EC Number
2.7.1.67

Proteins

phosphatidylinositol kinase-related protein kinase TOR2
Refseq ID NP_012719
Protein GI 42759860
UniProt ID P32600
mRNA ID NM_001179768
Length 2474
RefSeq Status PROVISIONAL
MNKYINKYTTPPNLLSLRQRAEGKHRTRKKLTHKSHSHDDEMSTTSNTDSNHNGPNDSGRVITGSAGHIGKISFVDSELDTTFSTLNLIFDKLKSDVPQERASGANELSTTLTSLAREVSAEQFQRFSNSLNNKIFELIHGFTSSEKIGGILAVDTLISFYLSTEELPNQTSRLANYLRVLIPSSDIEVMRLAANTLGRLTVPGGTLTSDFVEFEVRTCIDWLTLTADNNSSSSKLEYRRHAALLIIKALADNSPYLLYPYVNSILDNIWVPLRDAKLIIRLDAAVALGKCLTIIQDRDPALGKQWFQRLFQGCTHGLSLNTNDSVHATLLVFRELLSLKAPYLRDKYDDIYKSTMKYKEYKFDVIRREVYAILPLLAAFDPAIFTKKYLDRIMVHYLRYLKNIDMNAANNSDKPFILVSIGDIAFEVGSSISPYMTLILDNIREGLRTKFKVRKQFEKDLFYCIGKLACALGPAFAKHLNKDLLNLMLNCPMSDHMQETLMILNEKIPSLESTVNSRILNLLSISLSGEKFIQSNQYDFNNQFSIEKARKSRNQSFMKKTGESNDDITDAQILIQCFKMLQLIHHQYSLTEFVRLITISYIEHEDSSVRKLAALTSCDLFIKDDICKQTSVHALHSVSEVLSKLLMIAITDPVAEIRLEILQHLGSNFDPQLAQPDNLRLLFMALNDEIFGIQLEAIKIIGRLSSVNPAYVVPSLRKTLLELLTQLKFSNMPKKKEESATLLCTLINSSDEVAKPYIDPILDVILPKCQDASSAVASTALKVLGELSVVGGKEMTRYLKELMPLIINTFQDQSNSFKRDAALTTLGQLAASSGYVVGPLLDYPELLGILINILKTENNPHIRRGTVRLIGILGALDPYKHREIEVTSNSKSSVEQNAPSIDIALLMQGVSPSNDEYYPTVVIHNLMKILNDPSLSIHHTAAIQAIMHIFQNLGLRCVSFLDQIIPGIILVMRSCPPSQLDFYFQQLGSLISIVKQHIRPHVEKIYGVIREFFPIIKLQITIISVIESISKALEGEFKRFVPETLTFFLDILENDQSNKRIVPIRILKSLVTFGPNLEDYSHLIMPIVVRMTEYSAGSLKKISIITLGRLAKNINLSEMSSRIVQALVRILNNGDRELTKATMNTLSLLLLQLGTDFVVFVPVINKALLRNRIQHSVYDQLVNKLLNNECLPTNIIFDKENEVPERKNYEDEMQVTKLPVNQNILKNAWYCSQQKTKEDWQEWIRRLSIQLLKESPSACLRSCSSLVSVYYPLARELFNASFSSCWVELQTSYQEDLIQALCKALSSSENPPEIYQMLLNLVEFMEHDDKPLPIPIHTLGKYAQKCHAFAKALHYKEVEFLEEPKNSTIEALISINNQLHQTDSAIGILKHAQQHNELQLKETWYEKLQRWEDALAAYNEKEAAGEDSVEVMMGKLRSLYALGEWEELSKLASEKWGTAKPEVKKAMAPLAAGAAWGLEQWDEIAQYTSVMKSQSPDKEFYDAILCLHRNNFKKAEVHIFNARDLLVTELSALVNESYNRAYNVVVRAQIIAELEEIIKYKKLPQNSDKRLTMRETWNTRLLGCQKNIDVWQRILRVRSLVIKPKEDAQVRIKFANLCRKSGRMALAKKVLNTLLEETDDPDHPNTAKASPPVVYAQLKYLWATGLQDEALKQLINFTSRMAHDLGLDPNNMIAQSVPQQSKRVPRHVEDYTKLLARCFLKQGEWRVCLQPKWRLSNPDSILGSYLLATHFDNTWYKAWHNWALANFEVISMLTSVSKKKQEGSDASSVTDINEFDNGMIGVNTFDAKEVHYSSNLIHRHVIPAIKGFFHSISLSESSSLQDALRLLTLWFTFGGIPEATQAMHEGFNLIQIGTWLEVLPQLISRIHQPNQIVSRSLLSLLSDLGKAHPQALVYPLMVAIKSESLSRQKAALSIIEKMRIHSPVLVDQAELVSHELIRMAVLWHEQWYEGLDDASRQFFGEHNTEKMFAALEPLYEMLKRGPETLREISFQNSFGRDLNDAYEWLMNYKKSKDVSNLNQAWDIYYNVFRKIGKQLPQLQTLELQHVSPKLLSAHDLELAVPGTRASGGKPIVKISKFEPVFSVISSKQRPRKFCIKGSDGKDYKYVLKGHEDIRQDSLVMQLFGLVNTLLQNDAECFRRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREHREAKKIPLNIEHWVMLQMAPDYDNLTLLQKVEVFTYALNNTEGQDLYKVLWLKSRSSETWLERRTTYTRSLAVMSMTGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILREKFPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMKVLRDNKGSLMAILEAFAFDPLINWGFDLPTKKIEEETGIQLPVMNANELLSNGAITEEEVQRVENEHKNAIRNARAMLVLKRITDKLTGNDIRRFNDLDVPEQVDKLIQQATSVENLCQHYIGWCPFW

Gene Information

Entrez Gene ID
853632
Gene Name
phosphatidylinositol kinase-related protein kinase TOR2
Gene Symbol
TOR2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031931 IPI:SGD C TORC1 complex
GO:0031932 IPI:SGD C TORC2 complex
GO:0005737 IDA:SGD C cytoplasm
GO:0031234 IDA:SGD C extrinsic component of cytoplasmic side of plasma membrane
GO:0000329 IDA:SGD C fungal-type vacuole membrane
GO:0005886 IDA:SGD C plasma membrane
GO:0004430 IEA:UniProtKB-EC F 1-phosphatidylinositol 4-kinase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0008144 IEA:InterPro F drug binding
GO:0004674 IDA:SGD F protein serine/threonine kinase activity
GO:0031929 IMP:SGD P TOR signaling
GO:0030037 TAS:SGD P actin filament reorganization involved in cell cycle
GO:0007010 IMP:SGD P cytoskeleton organization
GO:0030950 IMP:SGD P establishment or maintenance of actin cytoskeleton polarity
GO:0010507 IGI:SGD P negative regulation of autophagy
GO:2001108 IMP:SGD P positive regulation of Rho guanyl-nucleotide exchange factor activity
GO:0035025 IMP:SGD P positive regulation of Rho protein signal transduction
GO:0045807 IMP:SGD P positive regulation of endocytosis
GO:0006468 IDA:GOC P protein phosphorylation
GO:0051726 TAS:SGD P regulation of cell cycle
GO:0001558 TAS:SGD P regulation of cell growth
GO:0042254 IMP:SGD P ribosome biogenesis
GO:0007165 TAS:SGD P signal transduction

KEGG Pathway Links

KEGG Pathway ID Description
sce04113 Meiosis - yeast

REACTOME Pathway Links

REACTOME Pathway ID Description
5618391 CD28 dependent PI3K/Akt signaling
5618646 Constitutive PI3K/AKT Signaling in Cancer
5618405 VEGFR2 mediated vascular permeability

Domain Information

InterPro Annotations

Accession Description
IPR011989 Armadillo-like helical
IPR016024 Armadillo-type fold
IPR024585 Domain of unknown function DUF3385, target of rapamycin protein
IPR014009 PIK-related kinase
IPR003151 PIK-related kinase, FAT
IPR003152 PIK-related kinase, FATC
IPR000403 Phosphatidylinositol 3-/4-kinase, catalytic domain
IPR018936 Phosphatidylinositol 3/4-kinase, conserved site
IPR011009 Protein kinase-like domain
IPR009076 Rapamycin-binding domain

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol kinase-related protein kinase TOR2
Protein Entry
TOR2_YEAST
UniProt ID
P32600
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate. {ECO:0000269|PubMed:8846782}.
Catalytic Activity ATP + a protein = ADP + a phosphoprotein. {ECO:0000269|PubMed:8846782}.
Function Phosphatidylinositol 3-kinase homolog, component of both TORC1 and TORC2. TORC1 regulates multiple cellular processes to control cell growth in response to environmental signals. Nutrient limitation and environmental stress signals cause inactivation of TORC1. Active TORC1 positively controls ribosome biogenesis via control of rRNA, ribosomal protein and tRNA gene expression, and rRNA processing. TORC1 positively controls protein biosynthesis by regulation of mRNA stability, translation initiation factor activity, and high-affinity amino acid permeases that serve to provide amino acids for use by the translation machinery. TORC1 also promotes growth by sequestering a number of nutrient and general stress-responsive transcription factors in the cytoplasm. TORC1 negatively controls macroautophagy, a process to recycle surplus cytoplasmic mass under nutrient starvation conditions. TORC1 controls many of these processes via TIP41-TAP42-mediated inhibition of the type 2A-related phosphatases PP2A and SIT4. TORC2 regulates cell cycle-dependent polarization of the actin- cytoskeleton, cell wall integrity, and receptor endocytosis. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway by activating the RHO1 guanine nucleotide exchange factor ROM2. TORC2 phosphorylates the AGC kinase YPK2, an upstream effector of the cell integrity pathway. TORC2 negatively regulates calcineurin-dependent stress signaling via phosphorylation of its effector SLM1-SLM2. {ECO:0000269|PubMed:10198052, ECO:0000269|PubMed:10329624, ECO:0000269|PubMed:10604478, ECO:0000269|PubMed:11741537, ECO:0000269|PubMed:14593073, ECO:0000269|PubMed:15372071, ECO:0000269|PubMed:15620355, ECO:0000269|PubMed:16055732, ECO:0000269|PubMed:16959779, ECO:0000269|PubMed:7606777, ECO:0000269|PubMed:8741837, ECO:0000269|PubMed:8846782, ECO:0000269|PubMed:8943012, ECO:0000269|PubMed:9539725, ECO:0000269|PubMed:9843498}.
Interaction Q08236:AVO1; NbExp=4; IntAct=EBI-19385, EBI-29284; Q04749:AVO2; NbExp=4; IntAct=EBI-19385, EBI-28131; P47041:BIT61; NbExp=2; IntAct=EBI-19385, EBI-25889; P41318:LST8; NbExp=7; IntAct=EBI-19385, EBI-28598; Q04372:TAP42; NbExp=4; IntAct=EBI-19385, EBI-18926; P40061:TSC11; NbExp=5; IntAct=EBI-19385, EBI-22621;
Similarity Belongs to the PI3/PI4-kinase family. {ECO:0000305}.
Similarity Contains 1 FAT domain. {ECO:0000255|PROSITE- ProRule:PRU00534}.
Similarity Contains 1 FATC domain. {ECO:0000255|PROSITE- ProRule:PRU00535}.
Similarity Contains 1 PI3K/PI4K domain. {ECO:0000255|PROSITE- ProRule:PRU00269}.
Similarity Contains 11 HEAT repeats. {ECO:0000305}.
Subcellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Vacuole membrane; Peripheral membrane protein; Cytoplasmic side. Note=Also localizes to membranous structures both proximal to, yet distinct from, the plasma membrane as well as within the cell interior, probably endosomal or Golgi membranes.
Subunit The target of rapamycin complex 1 (TORC1) is composed of at least KOG1, LST8, TCO89 and either TOR1 (TORC1-A) or TOR2 (TORC1-B). TORC1 binds to and is inhibited by FKBP-rapamycin. The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. Interacts with SLM1 and SLM2. {ECO:0000269|PubMed:12408816, ECO:0000269|PubMed:12631735, ECO:0000269|PubMed:15689497, ECO:0000269|PubMed:16002396, ECO:0000269|PubMed:16959779}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007158 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42759860 RefSeq NP_012719 2474 phosphatidylinositol kinase-related protein kinase TOR2

Identical Sequences to LMP007158 proteins

Reference Database Accession Length Protein Name
GI:42759860 EMBL CAA50548.1 2474 TOR2 [Saccharomyces cerevisiae]
GI:42759860 EMBL CAZ78118.1 2474 unnamed protein product [Saccharomyces cerevisiae]
GI:42759860 GenBank AAN97895.1 2474 Sequence 3 from patent US 6476200
GI:42759860 GenBank AAO93494.1 2474 Sequence 4 from patent US 6492106
GI:42759860 SwissProt P32600.3 2474 RecName: Full=Serine/threonine-protein kinase TOR2; AltName: Full=Dominant rapamycin resistance protein 2; AltName: Full=Phosphatidylinositol 4-kinase TOR2; Short=PI4-kinase TOR2; Short=PI4K TOR2; Short=PtdIns-4-kinase TOR2; AltName: Full=Target of rapamycin kinase 2; AltName: Full=Temperature-sensitive CSG2 suppressor protein 14 [Saccharomyces cerevisiae S288c]
GI:42759860 Third Party Genbank DAA08966.1 2474 TPA: phosphatidylinositol kinase-related protein kinase TOR2 [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007158 proteins

Reference Database Accession Length Protein Name
GI:42759860 DBBJ GAA24534.1 2474 K7_Tor2p [Saccharomyces cerevisiae Kyokai no. 7]
GI:42759860 EMBL CAA82048.1 2473 TOR2 [Saccharomyces cerevisiae]
GI:42759860 EMBL CAZ86717.1 2473 unnamed protein product [uncultured bacterium]
GI:42759860 GenBank ABZ48279.1 2473 Sequence 22217 from patent US 7314974
GI:42759860 GenBank EIW09244.1 2474 Tor2p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:42759860 PRF - 2474 TOR2(DRR2) gene [Saccharomyces cerevisiae]