Gene/Proteome Database (LMPD)
Proteins
| peroxiredoxin HYR1 | |
|---|---|
| Refseq ID | NP_012303 |
| Protein GI | 6322228 |
| UniProt ID | P40581 |
| mRNA ID | NM_001179559 |
| Length | 163 |
| MSEFYKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFTPQYKELEALYKRYKDEGFTIIGFPCNQFGHQEPGSDEEIAQFCQLNYGVTFPIMKKIDVNGGNEDPVYKFLKSQKSGMLGLRGIKWNFEKFLVDKKGKVYERYSSLTKPSSLSETIEELLKEVE | |
Gene Information
Entrez Gene ID
Gene Name
peroxiredoxin HYR1
Gene Symbol
Species
Saccharomyces cerevisiae S288c
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005622 | IPI:SGD | C | intracellular |
| GO:0005758 | IDA:SGD | C | mitochondrial intermembrane space |
| GO:0005782 | IDA:SGD | C | peroxisomal matrix |
| GO:0004602 | IDA:SGD | F | glutathione peroxidase activity |
| GO:0051920 | IEA:UniProtKB-EC | F | peroxiredoxin activity |
| GO:0047066 | IDA:SGD | F | phospholipid-hydroperoxide glutathione peroxidase activity |
| GO:0034599 | IMP:SGD | P | cellular response to oxidative stress |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko00590 | Arachidonic acid metabolism |
| sce00590 | Arachidonic acid metabolism |
| ko00480 | Glutathione metabolism |
| sce00480 | Glutathione metabolism |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | 2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH |
| Catalytic Activity | 2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH. {ECO:0000269|PubMed:18767166}. |
| Caution | Was originally thought to be a glutathione peroxidase or a phospholipid hydroperoxide glutathione peroxidase. HYP1 does not require glutathione for hydroperoxide reduction |
| Caution | Was originally thought to be a glutathione peroxidase or a phospholipid hydroperoxide glutathione peroxidase. HYP1 does not require glutathione for hydroperoxide reduction. {ECO:0000305}. |
| Function | Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYP1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYP1 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598') and a reduced Cys-36 in HYP1/GPX3. {ECO:0000269|PubMed:10480913, ECO:0000269|PubMed:11445588, ECO:0000269|PubMed:11875065, ECO:0000269|PubMed:12437921, ECO:0000269|PubMed:12517450, ECO:0000269|PubMed:12743123, ECO:0000269|PubMed:14556853, ECO:0000269|PubMed:9315326}. |
| Induction | In contrast to the other two peroxiredoxins, HYP1/GPX3 expression is constitutive, not stress-induced |
| Induction | In contrast to the other two peroxiredoxins, HYP1/GPX3 expression is constitutive, not stress-induced. {ECO:0000269|PubMed:10480913}. |
| Interaction | P38009:ADE17; NbExp=1; IntAct=EBI-7869, EBI-14223; P54783:ALO1; NbExp=1; IntAct=EBI-7869, EBI-2519; P07991:CAR2; NbExp=1; IntAct=EBI-7869, EBI-12430; P10614:ERG11; NbExp=1; IntAct=EBI-7869, EBI-5127; P19145:GAP1; NbExp=1; IntAct=EBI-7869, EBI-7314; P32288:GLN1; NbExp=1; IntAct=EBI-7869, EBI-7665; P39004:HXT7; NbExp=1; IntAct=EBI-7869, EBI-8790; Q02785:PDR12; NbExp=1; IntAct=EBI-7869, EBI-13065; Q12462:PEX11; NbExp=1; IntAct=EBI-7869, EBI-13198; P40975:PMP2; NbExp=1; IntAct=EBI-7869, EBI-2043041; P87284:PMP3; NbExp=1; IntAct=EBI-7869, EBI-13555; P09938:RNR2; NbExp=1; IntAct=EBI-7869, EBI-15240; P0C268:YBL039W-B; NbExp=1; IntAct=EBI-7869, EBI-2044092; |
| Miscellaneous | Present with 8000 molecules/cell in log phase SD medium |
| Miscellaneous | Present with 8000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Ptm | Reversible disulfide bond formation between Cys-36 and Cys-82 upon peroxide reduction; probably reverted by thioredoxin (TRX2). |
| Similarity | Belongs to the glutathione peroxidase family |
| Similarity | Belongs to the glutathione peroxidase family. {ECO:0000305}. |
| Subcellular Location | Cytoplasm. Mitochondrion intermembrane space. Peroxisome matrix. |
| Subunit | Interacts with YAP1 and probably YBP1 |
| Subunit | Interacts with YAP1 and probably YBP1. {ECO:0000269|PubMed:12743123}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007140 (as displayed in Record Overview)
Identical Sequences to LMP007140 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|
Related Sequences to LMP007140 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|