Gene/Proteome Database (LMPD)

LMPD ID
LMP007137
Gene ID
854682
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
acylglycerone-phosphate reductase
Gene Symbol
AYR1
Synonyms
GBG1
Alternate Names
acylglycerone-phosphate reductase
Chromosome
IX
EC Number
1.1.1.101

Proteins

acylglycerone-phosphate reductase
Refseq ID NP_012142
Protein GI 398364253
UniProt ID P40471
mRNA ID NM_001179472
Length 297
RefSeq Status PROVISIONAL
MSELQSQPKKIAVVTGASGGIGYEVTKELARNGYLVYACARRLEPMAQLAIQFGNDSIKPYKLDISKPEEIVTFSGFLRANLPDGKLDLLYNNAGQSCTFPALDATDAAVEQCFKVNVFGHINMCRELSEFLIKAKGTIVFTGSLAGVVSFPFGSIYSASKAAIHQYARGLHLEMKPFNVRVINAITGGVATDIADKRPLPETSIYNFPEGREAFNSRKTMAKDNKPMPADAYAKQLVKDILSTSDPVDVYRGTFANIMRFVMIFVPYWLLEKGLSKKFKLDKVNNALKSKQKNKDD

Gene Information

Entrez Gene ID
854682
Gene Name
acylglycerone-phosphate reductase
Gene Symbol
AYR1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:SGD C endoplasmic reticulum
GO:0005811 IDA:SGD C lipid particle
GO:0000140 IMP:SGD F acylglycerone-phosphate reductase activity
GO:0004806 IDA:SGD F triglyceride lipase activity
GO:0006654 IMP:SGD P phosphatidic acid biosynthetic process
GO:0019433 IGI:SGD P triglyceride catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
sce00565 Ether lipid metabolism
sce00564 Glycerophospholipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-7411 superpathway phosphatidate biosynthesis (yeast)

Domain Information

InterPro Annotations

Accession Description
IPR002347 Glucose/ribitol dehydrogenase
IPR016040 NAD(P)-binding domain
IPR002198 Short-chain dehydrogenase/reductase SDR
IPR020904 Short-chain dehydrogenase/reductase, conserved site

UniProt Annotations

Entry Information

Gene Name
acylglycerone-phosphate reductase
Protein Entry
AYR1_YEAST
UniProt ID
P40471
Species
Yeast (S288c)

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=20 uM for NADPH {ECO:0000269|PubMed:1512203}; KM=15 uM for hexadecyl dihydroxyacetone-phosphate {ECO:0000269|PubMed:1512203}; Vmax=3.8 nmol/min/mg enzyme for hexadecyl dihydroxyacetone- phosphate {ECO:0000269|PubMed:1512203}; pH dependence: Optimum pH is 6.7-7.2. {ECO:0000269|PubMed:1512203}; Temperature dependence: Optimum temperature is 40 degrees Celsius. Thermostable for 10 min up to 45 degrees Celsius. {ECO:0000269|PubMed:1512203};
Catalytic Activity 1-palmitoylglycerol 3-phosphate + NADP(+) = palmitoylglycerone phosphate + NADPH.
Enzyme Regulation Inhibited by divalent cations and N- ethylmaleimide. Activity is reduced under anaerobic growth conditions. {ECO:0000269|PubMed:1512203}.
Function Can convert acyl and alkyl dihydroxyacetone-phosphate (DHAP) into glycerolipids and ether lipids, respectively. Required for the biosynthesis of phosphatidic acid via the DHAP pathway, where it reduces 1-acyl DHAP to lysophosphatidic acid (LPA). Required for spore germination. {ECO:0000269|PubMed:10617610}.
Miscellaneous Present with 3671 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Similarity Belongs to the short-chain dehydrogenases/reductases (SDR) family. {ECO:0000305}.
Subcellular Location Lipid droplet. Endoplasmic reticulum.

Identical and Related Proteins

Unique RefSeq proteins for LMP007137 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398364253 RefSeq NP_012142 297 acylglycerone-phosphate reductase

Identical Sequences to LMP007137 proteins

Reference Database Accession Length Protein Name
GI:398364253 GenBank EDN61377.1 297 1-acyl dihydroxyacetone phosphate reductase [Saccharomyces cerevisiae YJM789]
GI:398364253 GenBank EWG85286.1 297 Ayr1p [Saccharomyces cerevisiae R008]
GI:398364253 GenBank EWG90457.1 297 Ayr1p [Saccharomyces cerevisiae P301]
GI:398364253 GenBank EWH17860.1 297 Ayr1p [Saccharomyces cerevisiae P283]
GI:398364253 gnl McCuskerlabDuke 297 Ayr1p [Saccharomyces cerevisiae YJM993]
GI:398364253 Third Party Genbank DAA08429.1 297 TPA: acylglycerone-phosphate reductase [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007137 proteins

Reference Database Accession Length Protein Name
GI:398364253 DBBJ GAA23996.1 297 K7_Ayr1p [Saccharomyces cerevisiae Kyokai no. 7]
GI:398364253 EMBL CAY80383.1 297 Ayr1p [Saccharomyces cerevisiae EC1118]
GI:398364253 GenBank AAZ22503.1 297 Ayr1p [Saccharomyces cerevisiae]
GI:398364253 GenBank EDV09602.1 297 1-acyl dihydroxyacetone phosphate reductase [Saccharomyces cerevisiae RM11-1a]
GI:398364253 GenBank EIW09889.1 297 Ayr1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:398364253 GenBank EWG95424.1 297 Ayr1p [Saccharomyces cerevisiae R103]