LIPID MAPS

LMPD ID

LMP007135

Gene ID

850892

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

glycylpeptide N-tetradecanoyltransferase NMT1

Gene Symbol

NMT1

Synonyms

CDC72

Chromosome

XII

EC Number

2.3.1.97

glycylpeptide N-tetradecanoyltransferase NMT1
Refseq ID	NP_013296
Protein GI	6323224
UniProt ID	P14743
mRNA ID	NM_001182082
Length	455
MSEEDKAKKLENLLKLLQLNNDDTSKFTQEQKKAMKDHKFWRTQPVKDFDEKVVEEGPIDKPKTPEDISDKPLPLLSSFEWCSIDVDNKKQLEDVFVLLNENYVEDRDAGFRFNYTKEFFNWALKSPGWKKDWHIGVRVKETQKLVAFISAIPVTLGVRGKQVPSVEINFLCVHKQLRSKRLTPVLIKEITRRVNKCDIWHALYTAGIVLPAPVSTCRYTHRPLNWKKLYEVDFTGLPDGHTEEDMIAENALPAKTKTAGLRKLKKEDIDQVFELFKRYQSRFELIQIFTKEEFEHNFIGEESLPLDKQVIFSYVVEQPDGKITDFFSFYSLPFTILNNTKYKDLGIGYLYYYATDADFQFKDRFDPKATKALKTRLCELIYDACILAKNANMDVFNALTSQDNTLFLDDLKFGPGDGFLNFYLFNYRAKPITGGLNPDNSNDIKRRSNVGVVML

Entrez Gene ID

850892

Gene Name

glycylpeptide N-tetradecanoyltransferase NMT1

Gene Symbol

NMT1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005829	IDA:SGD	C	cytosol
GO:0004379	IMP:SGD	F	glycylpeptide N-tetradecanoyltransferase activity
GO:0018008	TAS:SGD	P	N-terminal peptidyl-glycine N-myristoylation
GO:0001302	IMP:SGD	P	replicative cell aging

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618026	Disease
5618651	Diseases associated with visual transduction
5618659	Inactivation, recovery and regulation of the phototransduction cascade
5618100	Signal Transduction
5618660	The phototransduction cascade
5618653	Visual phototransduction

InterPro Annotations

Accession	Description
IPR016181	Acyl-CoA N-acyltransferase
IPR000903	Myristoyl-CoA:protein N-myristoyltransferase
IPR022677	Myristoyl-CoA:protein N-myristoyltransferase, C-terminal
IPR022676	Myristoyl-CoA:protein N-myristoyltransferase, N-terminal
IPR022678	Myristoyl-CoA:protein N-myristoyltransferase, conserved site

Entry Information

Gene Name

glycylpeptide N-tetradecanoyltransferase NMT1

Protein Entry

NMT_YEAST

UniProt ID

P14743

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=1.4 uM for myristoyl-CoA {ECO:0000269\|PubMed:11478885, ECO:0000269\|PubMed:3106975}; pH dependence: Optimum pH is 7.5-8.0. {ECO:0000269\|PubMed:11478885, ECO:0000269\|PubMed:3106975};
Biophysicochemical Properties	Kinetic parameters: KM=1.4 uM for myristoyl-CoA {ECO:0000269\|PubMed:11478885, ECO:0000269\|PubMed:3106975}; pH dependence: Optimum pH is 7.5-8.0. {ECO:0000269\|PubMed:11478885, ECO:0000269\|PubMed:3106975};
Catalytic Activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N- tetradecanoylglycylpeptide.
Enzyme Regulation	Inhibited by diethylpyrocarbonate. Competitively inhibited by S-(2-oxo)pentadecyl-CoA, a non hydrolysable myristoyl-CoA analog, and by SC-58272, a peptidomimetic derived from the N-terminal sequence of a natural substrate.
Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins. Substrate specificity requires an N- terminal glycine in the nascent polypeptide substrates. Uncharged amino acids are preferred at position 2 while neutral residues are favored at positions 3 and 4. Ser is present at position 5 in almost all known N-myristoyl proteins and Lys is commonly encountered at postion 6.
Miscellaneous	Has an ordered Bi-Bi kinetic mechanism, with myristoyl-CoA binding taking place prior to peptide binding and CoA release occurring before acylated peptide release. Cooperative interactions between the acyl-CoA and peptide binding sites of NMT contribute to its extraordinary chain-length specificity.
Ptm	The N-terminus is blocked.
Similarity	Belongs to the NMT family
Similarity	Belongs to the NMT family. {ECO:0000305}.
Subcellular Location	Cytoplasm .
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:14562095}.
Subunit	Monomer. {ECO:0000269\|PubMed:11371195, ECO:0000269\|PubMed:9846880}.