LIPID MAPS

LMPD ID

LMP007131

Gene ID

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

pyruvate carboxylase 1

Gene Symbol

PYC1

Synonyms

-

Chromosome

VII

EC Number

6.4.1.1

pyruvate carboxylase 1
Refseq ID	NP_011453
Protein GI	6321376
UniProt ID	P11154
mRNA ID	NM_001180927
Length	1178
MSQRKFAGLRDNFNLLGEKNKILVANRGEIPIRIFRTAHELSMQTVAIYSHEDRLSTHKQKADEAYVIGEVGQYTPVGAYLAIDEIISIAQKHQVDFIHPGYGFLSENSEFADKVVKAGITWIGPPAEVIDSVGDKVSARNLAAKANVPTVPGTPGPIETVEEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCFVERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAILTDAVKLAKECGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGASLPQLGLFQDKITTRGFAIQCRITTEDPAKNFQPDTGRIEVYRSAGGNGVRLDGGNAYAGTIISPHYDSMLVKCSCSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLLTNPVFIEGTYWTTFIDDTPQLFQMVSSQNRAQKLLHYLADVAVNGSSIKGQIGLPKLKSNPSVPHLHDAQGNVINVTKSAPPSGWRQVLLEKGPAEFARQVRQFNGTLLMDTTWRDAHQSLLATRVRTHDLATIAPTTAHALAGRFALECWGGATFDVAMRFLHEDPWERLRKLRSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLKVGVDAVKKAGGVVEATVCFSGDMLQPGKKYNLDYYLEIAEKIVQMGTHILGIKDMAGTMKPAAAKLLIGSLRAKYPDLPIHVHTHDSAGTAVASMTACALAGADVVDVAINSMSGLTSQPSINALLASLEGNIDTGINVEHVRELDAYWAEMRLLYSCFEADLKGPDPEVYQHEIPGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVSNKLTSDDVRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPFRSDVLRNKRRKLTCRPGLELEPFDLEKIREDLQNRFGDVDECDVASYNMYPRVYEDFQKMRETYGDLSVLPTRSFLSPLETDEEIEVVIEQGKTLIIKLQAVGDLNKKTGEREVYFDLNGEMRKIRVADRSQKVETVTKSKADMHDPLHIGAPMAGVIVEVKVHKGSLIKKGQPVAVLSAMKMEMIISSPSDGQVKEVFVSDGENVDSSDLLVLLEDQVPVETKA

Entrez Gene ID

Gene Name

pyruvate carboxylase 1

Gene Symbol

PYC1

Species

Saccharomyces cerevisiae S288c

GO ID	Source	Type	Description
GO:0005829	IDA:SGD	C	cytosol
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0003677	IEA:InterPro	F	DNA binding
GO:0004075	IEA:InterPro	F	biotin carboxylase activity
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0004736	IMP:SGD	F	pyruvate carboxylase activity
GO:0006094	IMP:SGD	P	gluconeogenesis

BIOCYC Pathway ID	Description
TCA-EUK-PWY-YEAST	TCA cycle, aerobic respiration
ASPBIO-PWY	aspartate biosynthesis
GLUCONEO-YEAST-PWY	gluconeogenesis

REACTOME Pathway ID	Description
5618669	Biotin transport and metabolism
5618374	Defective AMN causes hereditary megaloblastic anemia 1
5618384	Defective BTD causes biotidinase deficiency
5618383	Defective CD320 causes methylmalonic aciduria
5618375	Defective CUBN causes hereditary megaloblastic anemia 1
5618372	Defective GIF causes intrinsic factor deficiency
5618386	Defective HLCS causes multiple carboxylase deficiency
5618373	Defective LMBRD1 causes methylmalonic aciduria and homocystinuria type cblF
5618381	Defective MMAA causes methylmalonic aciduria type cblA
5618378	Defective MMAB causes methylmalonic aciduria type cblB
5618380	Defective MMACHC causes methylmalonic aciduria and homocystinuria type cblC
5618379	Defective MMADHC causes methylmalonic aciduria and homocystinuria type cblD
5618377	Defective MTR causes methylmalonic aciduria and homocystinuria type cblG
5618376	Defective MTRR causes methylmalonic aciduria and homocystinuria type cblE
5618382	Defective MUT causes methylmalonic aciduria mut type
5618369	Defective TCN2 causes hereditary megaloblastic anemia
5618385	Defects in biotin (Btn) metabolism
5618370	Defects in cobalamin (B12) metabolism
5618371	Defects in vitamin and cofactor metabolism
5618026	Disease
5618020	Gluconeogenesis
5618021	Glucose metabolism
5618025	Glycogen storage diseases
5618023	Metabolism
5618022	Metabolism of carbohydrates
5618368	Metabolism of vitamins and cofactors
5618367	Metabolism of water-soluble vitamins and cofactors
5618024	Myoclonic epilepsy of Lafora

Accession	Description
IPR011761	ATP-grasp fold
IPR013815	ATP-grasp fold, subdomain 1
IPR013816	ATP-grasp fold, subdomain 2
IPR013785	Aldolase-type TIM barrel
IPR005482	Biotin carboxylase, C-terminal
IPR011764	Biotin carboxylation domain
IPR001882	Biotin-binding site
IPR000089	Biotin/lipoyl attachment
IPR005481	Carbamoyl-phosphate synthase, large subunit, N-terminal
IPR005479	Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
IPR003379	Carboxylase, conserved domain
IPR009057	Homeodomain-like
IPR016185	Pre-ATP-grasp domain
IPR005930	Pyruvate carboxylase
IPR000891	Pyruvate carboxyltransferase
IPR011054	Rudiment single hybrid motif
IPR011053	Single hybrid motif

Gene Name

pyruvate carboxylase 1

Protein Entry

PYC1_YEAST

UniProt ID

Species

Yeast (S288c)

Comment Type	Description
Catalytic Activity	ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate.
Cofactor	Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Cofactor	Name=biotin; Xref=ChEBI:CHEBI:57586; Note=Biotin.;
Function	Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Interaction	P39940:RSP5; NbExp=2; IntAct=EBI-14358, EBI-16219;
Miscellaneous	Present with 12500 molecules/cell in log phase SD medium
Miscellaneous	Present with 12500 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Similarity	Contains 1 ATP-grasp domain. {ECO:0000255\|PROSITE- ProRule:PRU00409}.
Similarity	Contains 1 biotin carboxylation domain
Similarity	Contains 1 biotin carboxylation domain. {ECO:0000305}.
Similarity	Contains 1 biotinyl-binding domain
Similarity	Contains 1 biotinyl-binding domain. {ECO:0000255\|PROSITE-ProRule:PRU01066, ECO:0000305}.
Similarity	Contains 1 carboxyltransferase domain
Similarity	Contains 1 carboxyltransferase domain. {ECO:0000305}.
Subcellular Location	Cytoplasm.
Subunit	Homotetramer.