LIPID MAPS

LMPD ID

LMP007046

Gene ID

853861

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

sphingosine N-acyltransferase LAC1

Gene Symbol

LAC1

Synonyms

DGT1

Chromosome

XI

EC Number

2.3.1.24;

sphingosine N-acyltransferase LAC1
Refseq ID	NP_012917
Protein GI	398364795
UniProt ID	P28496
mRNA ID	NM_001179574
Length	418
MSTIKPSPSNNNLKVRSRPRRKSSIGKIDLGDTVPSLGTMFETKESKTAAKRRMQRLSEATKNDSDLVKKIWFSFREISYRHAWIAPLMILIAVYSAYFTSGNTTKTNVLHRFVAVSYQIGDTNAYGKGINDLCFVFYYMIFFTFLREFLMDVVIRPFAIRLHVTSKHRIKRIMEQMYAIFYTGVSGPFGIYCMYHSDLWFFNTKAMYRTYPDFTNPFLFKVFYLGQAAFWAQQACILVLQLEKPRKDHNELTFHHIVTLLLIWSSYVFHFTKMGLPIYITMDVSDFLLSFSKTLNYLDSGLAFFSFAIFVVAWIYLRHYINLKILWSVLTQFRTEGNYVLNFATQQYKCWISLPIVFVLIGALQLVNLYWLFLIFRVLYRILWRGILKDDRSDSESDEESDESSTTPTDSTPTKKDI

Entrez Gene ID

853861

Gene Name

sphingosine N-acyltransferase LAC1

Gene Symbol

LAC1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0061576	IDA:SGD	C	acyl-CoA ceramide synthase complex
GO:0005783	TAS:SGD	C	endoplasmic reticulum
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0050291	IDA:SGD	F	sphingosine N-acyltransferase activity
GO:0046513	IDA:SGD	P	ceramide biosynthetic process
GO:0001302	IGI:SGD	P	replicative cell aging

KEGG Pathway Links

KEGG Pathway ID	Description
sce01100	Metabolic pathways
ko00600	Sphingolipid metabolism
sce00600	Sphingolipid metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
SPHINGOLIPID-SYN-PWY	sphingolipid biosynthesis
SPHINGOLIPID-SYN-PWY	sphingolipid biosynthesis (yeast)

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618508	Sphingolipid de novo biosynthesis
5618509	Sphingolipid metabolism

InterPro Annotations

Accession	Description
IPR016439	Longevity assurance, LAG1/LAC1
IPR006634	TRAM/LAG1/CLN8 homology domain
IPR013599	TRAM1

Entry Information

Gene Name

sphingosine N-acyltransferase LAC1

Protein Entry

LAC1_YEAST

UniProt ID

P28496

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Acyl-CoA + sphingosine = CoA + N- acylsphingosine.
Function	Component of the ceramide synthase complex required for C26-CoA-dependent ceramide synthesis. Redundant with LAG1. Facilitates ER-to-Golgi transport of GPI-anchored proteins. {ECO:0000269\|PubMed:10198056, ECO:0000269\|PubMed:11387200, ECO:0000269\|PubMed:11694577, ECO:0000269\|PubMed:15692566}.
Induction	Expression is controlled by PDR1 and PDR3 which bind its propmoter PDRE element, and by ROX1 and CBF1
Induction	Expression is controlled by PDR1 and PDR3 which bind its propmoter PDRE element, and by ROX1 and CBF1. {ECO:0000269\|PubMed:15302821}.
Interaction	P38703:LAG1; NbExp=7; IntAct=EBI-26585, EBI-10035; Q03579:LIP1; NbExp=6; IntAct=EBI-26585, EBI-27640;
Miscellaneous	Present with 2840 molecules/cell in log phase SD medium
Miscellaneous	Present with 2840 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the sphingosine N-acyltransferase family
Similarity	Belongs to the sphingosine N-acyltransferase family. {ECO:0000305}.
Similarity	Contains 1 TLC (TRAM/LAG1/CLN8) domain
Similarity	Contains 1 TLC (TRAM/LAG1/CLN8) domain. {ECO:0000255\|PROSITE-ProRule:PRU00205}.
Subcellular Location	Endoplasmic reticulum membrane ; Multi- pass membrane protein {ECO:0000269\|PubMed:10198056, ECO:0000269\|PubMed:14562095}.
Subcellular Location	Endoplasmic reticulum membrane {ECO:0000269\|PubMed:10198056, ECO:0000269\|PubMed:14562095}; Multi- pass membrane protein {ECO:0000269\|PubMed:10198056, ECO:0000269\|PubMed:14562095}.
Subunit	Composed of the ceramide synthase complex composed of at least LAC1, LAG1 and LIP1.