LIPID MAPS

LMPD ID

LMP007015

Gene ID

853086

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

squalene monooxygenase

Gene Symbol

ERG1

Synonyms

-

Chromosome

VII

EC Number

1.14.13.132

squalene monooxygenase
Refseq ID	NP_011691
Protein GI	6321614
UniProt ID	P32476
mRNA ID	NM_001181304
Length	496
MSAVNVAPELINADNTITYDAIVIGAGVIGPCVATGLARKGKKVLIVERDWAMPDRIVGELMQPGGVRALRSLGMIQSINNIEAYPVTGYTVFFNGEQVDIPYPYKADIPKVEKLKDLVKDGNDKVLEDSTIHIKDYEDDERERGVAFVHGRFLNNLRNITAQEPNVTRVQGNCIEILKDEKNEVVGAKVDIDGRGKVEFKAHLTFICDGIFSRFRKELHPDHVPTVGSSFVGMSLFNAKNPAPMHGHVILGSDHMPILVYQISPEETRILCAYNSPKVPADIKSWMIKDVQPFIPKSLRPSFDEAVSQGKFRAMPNSYLPARQNDVTGMCVIGDALNMRHPLTGGGMTVGLHDVVLLIKKIGDLDFSDREKVLDELLDYHFERKSYDSVINVLSVALYSLFAADSDNLKALQKGCFKYFQRGGDCVNKPVEFLSGVLPKPLQLTRVFFAVAFYTIYLNMEERGFLGLPMALLEGIMILITAIRVFTPFLFGELIG

Entrez Gene ID

853086

Gene Name

squalene monooxygenase

Gene Symbol

ERG1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005783	IDA:SGD	C	endoplasmic reticulum
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0005811	IDA:SGD	C	lipid particle
GO:0008144	IMP:SGD	F	drug binding
GO:0050660	IEA:InterPro	F	flavin adenine dinucleotide binding
GO:0004506	IDA:SGD	F	squalene monooxygenase activity
GO:0006696	IMP:SGD	P	ergosterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
sce01110	Biosynthesis of secondary metabolites
sce01100	Metabolic pathways
ko00909	Sesquiterpenoid and triterpenoid biosynthesis
sce00909	Sesquiterpenoid and triterpenoid biosynthesis
ko00100	Steroid biosynthesis
sce00100	Steroid biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-5670	epoxysqualene biosynthesis
PWY-5670	epoxysqualene biosynthesis
ERGOSTEROL-SYN-PWY	superpathway of ergosterol biosynthesis
ERGOSTEROL-SYN-PWY	superpathway of ergosterol biosynthesis I

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618606	Activation of gene expression by SREBF (SREBP)
5618346	Cholesterol biosynthesis
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618607	Regulation of cholesterol biosynthesis by SREBP (SREBF)

InterPro Annotations

Accession	Description
IPR003042	Aromatic-ring hydroxylase-like
IPR013698	Squalene_epoxidase

Entry Information

Gene Name

squalene monooxygenase

Protein Entry

ERG1_YEAST

UniProt ID

P32476

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	Squalene + NADPH + O(2) = (3S)-2,3-epoxy-2,3- dihydrosqualene + NADP(+) + H(2)O.
Cofactor	Name=FAD; Xref=ChEBI:CHEBI:57692;
Enzyme Regulation	Inhibited by the allylamine antimycotic drugs.
Function	Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Interaction	P35206:CSG2; NbExp=1; IntAct=EBI-6545, EBI-2051140; P53045:ERG25; NbExp=1; IntAct=EBI-6545, EBI-6506; P25340:ERG4; NbExp=1; IntAct=EBI-6545, EBI-6558; Q05359:ERP1; NbExp=1; IntAct=EBI-6545, EBI-6581; Q12450:ERP4; NbExp=1; IntAct=EBI-6545, EBI-6598; P54837:ERV25; NbExp=1; IntAct=EBI-6545, EBI-6642; P53337:ERV29; NbExp=1; IntAct=EBI-6545, EBI-23662; Q03554:GOT1; NbExp=1; IntAct=EBI-6545, EBI-2057821; P06197:PIS1; NbExp=1; IntAct=EBI-6545, EBI-13458; P14359:SNA3; NbExp=1; IntAct=EBI-6545, EBI-26122; Q02795:SWP1; NbExp=1; IntAct=EBI-6545, EBI-12666; P38845:YHR146W; NbExp=1; IntAct=EBI-6545, EBI-24770; Q02256:YVH1; NbExp=1; IntAct=EBI-6545, EBI-14322;
Miscellaneous	Present with 65400 molecules/cell in log phase SD medium
Miscellaneous	Present with 65400 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.
Similarity	Belongs to the squalene monooxygenase family
Similarity	Belongs to the squalene monooxygenase family. {ECO:0000305}.
Subcellular Location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Subunit	Interacts with ERG28
Subunit	Interacts with ERG28. {ECO:0000269\|PubMed:15995173}.