LIPID MAPS

LMPD ID

LMP006967

Gene ID

850846

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

acetate--CoA ligase ACS2

Gene Symbol

ACS2

Synonyms

-

Chromosome

XII

EC Number

6.2.1.1

acetate--CoA ligase ACS2
Refseq ID	NP_013254
Protein GI	6323182
UniProt ID	P52910
mRNA ID	NM_001182040
Length	683
MTIKEHKVVYEAHNVKALKAPQHFYNSQPGKGYVTDMQHYQEMYQQSINEPEKFFDKMAKEYLHWDAPYTKVQSGSLNNGDVAWFLNGKLNASYNCVDRHAFANPDKPALIYEADDESDNKIITFGELLRKVSQIAGVLKSWGVKKGDTVAIYLPMIPEAVIAMLAVARIGAIHSVVFAGFSAGSLKDRVVDANSKVVITCDEGKRGGKTINTKKIVDEGLNGVDLVSRILVFQRTGTEGIPMKAGRDYWWHEEAAKQRTYLPPVSCDAEDPLFLLYTSGSTGSPKGVVHTTGGYLLGAALTTRYVFDIHPEDVLFTAGDVGWITGHTYALYGPLTLGTASIIFESTPAYPDYGRYWRIIQRHKATHFYVAPTALRLIKRVGEAEIAKYDTSSLRVLGSVGEPISPDLWEWYHEKVGNKNCVICDTMWQTESGSHLIAPLAGAVPTKPGSATVPFFGINACIIDPVTGVELEGNDVEGVLAVKSPWPSMARSVWNHHDRYMDTYLKPYPGHYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTSEIEASISNHENVSEAAVVGIPDELTGQTVVAYVSLKDGYLQNNATEGDAEHITPDNLRRELILQVRGEIGPFASPKTIILVRDLPRTRSGKIMRRVLRKVASNEAEQLGDLTTLANPEVVPAIISAVENQFFSQKKK

Entrez Gene ID

850846

Gene Name

acetate--CoA ligase ACS2

Gene Symbol

ACS2

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005829	IDA:SGD	C	cytosol
GO:0005730	IDA:SGD	C	nucleolus
GO:0005634	IDA:SGD	C	nucleus
GO:0016208	IEA:InterPro	F	AMP binding
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0003987	IDA:SGD	F	acetate-CoA ligase activity
GO:0016880	IDA:SGD	F	acid-ammonia (or amide) ligase activity
GO:0006085	IDA:SGD	P	acetyl-CoA biosynthetic process
GO:0019427	IEA:InterPro	P	acetyl-CoA biosynthetic process from acetate
GO:0016573	IMP:SGD	P	histone acetylation
GO:0006090	IEA:UniProtKB-UniPathway	P	pyruvate metabolic process
GO:0001302	IMP:SGD	P	replicative cell aging

KEGG Pathway Links

KEGG Pathway ID	Description
sce01110	Biosynthesis of secondary metabolites
ko01200	Carbon metabolism
sce01200	Carbon metabolism
ko00010	Glycolysis / Gluconeogenesis
sce00010	Glycolysis / Gluconeogenesis
sce01100	Metabolic pathways
ko00680	Methane metabolism
sce00680	Methane metabolism
ko00640	Propanoate metabolism
sce00640	Propanoate metabolism
ko00620	Pyruvate metabolism
sce00620	Pyruvate metabolism

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY0-1313	acetate conversion to acetyl-CoA
PWY0-1313	acetate conversion to acetyl-CoA
PWY66-21	ethanol degradation II
PWY66-162	ethanol degradation IV

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618031	Biological oxidations
5618063	Ethanol oxidation
5618023	Metabolism
5618064	Phase 1 - Functionalization of compounds

InterPro Annotations

Accession	Description
IPR025110	AMP-binding enzyme C-terminal domain
IPR020845	AMP-binding, conserved site
IPR000873	AMP-dependent synthetase/ligase
IPR011904	Acetate-CoA ligase

Entry Information

Gene Name

acetate--CoA ligase ACS2

Protein Entry

ACS2_YEAST

UniProt ID

P52910

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=8.8 mM for acetate {ECO:0000269\|PubMed:8910545}; KM=1.3 mM for ATP {ECO:0000269\|PubMed:8910545}; Vmax=0.34 umol/min/mg enzyme {ECO:0000269\|PubMed:8910545};
Biophysicochemical Properties	Kinetic parameters: KM=8.8 mM for acetate ; KM=1.3 mM for ATP ; Vmax=0.34 umol/min/mg enzyme ;
Catalytic Activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.
Function	Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. "Anaerobic" isozyme of acetyl-coenzyme A synthetase, which is required for growth on fermentable carbon sources such as glucose. May be involved in the PDH (pyruvate dehydrogenase complex) bypass
Function	Catalyzes the production of acetyl-CoA. Provides the acetyl-CoA source for histone acetylation in the nucleus. "Anaerobic" isozyme of acetyl-coenzyme A synthetase, which is required for growth on fermentable carbon sources such as glucose. May be involved in the PDH (pyruvate dehydrogenase complex) bypass. {ECO:0000269\|PubMed:16857587, ECO:0000269\|PubMed:8910545}.
Miscellaneous	Present with 225000 molecules/cell in log phase SD medium
Miscellaneous	Present with 225000 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Carbohydrate metabolism; pyruvate metabolism.
Similarity	Belongs to the ATP-dependent AMP-binding enzyme family
Similarity	Belongs to the ATP-dependent AMP-binding enzyme family. {ECO:0000305}.
Subcellular Location	Cytoplasm. Nucleus. Note=Predominantly nuclear.