Gene/Proteome Database (LMPD)

LMPD ID
LMP006945
Gene ID
856925
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
Gene Symbol
PDA1
Synonyms
-
Alternate Names
pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
Chromosome
V
EC Number
1.2.4.1

Proteins

pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
Refseq ID NP_011105
Protein GI 398365125
UniProt ID P16387
mRNA ID NM_001179068
Length 420
RefSeq Status PROVISIONAL
MLAASFKRQPSQLVRGLGAVLRTPTRIGHVRTMATLKTTDKKAPEDIEGSDTVQIELPESSFESYMLEPPDLSYETSKATLLQMYKDMVIIRRMEMACDALYKAKKIRGFCHLSVGQEAIAVGIENAITKLDSIITSYRCHGFTFMRGASVKAVLAELMGRRAGVSYGKGGSMHLYAPGFYGGNGIVGAQVPLGAGLAFAHQYKNEDACSFTLYGDGASNQGQVFESFNMAKLWNLPVVFCCENNKYGMGTAASRSSAMTEYFKRGQYIPGLKVNGMDILAVYQASKFAKDWCLSGKGPLVLEYETYRYGGHSMSDPGTTYRTRDEIQHMRSKNDPIAGLKMHLIDLGIATEAEVKAYDKSARKYVDEQVELADAAPPPEAKLSILFEDVYVKGTETPTLRGRIPEDTWDFKKQGFASRD

Gene Information

Entrez Gene ID
856925
Gene Name
pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
Gene Symbol
PDA1
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0042645 IDA:SGD C mitochondrial nucleoid
GO:0005967 IDA:SGD C mitochondrial pyruvate dehydrogenase complex
GO:0005739 IDA:SGD C mitochondrion
GO:0004739 IMP:SGD F pyruvate dehydrogenase (acetyl-transferring) activity
GO:0006086 IDA:SGD P acetyl-CoA biosynthetic process from pyruvate
GO:0006096 IEA:UniProtKB-KW P glycolytic process
GO:0007124 IMP:SGD P pseudohyphal growth

KEGG Pathway Links

KEGG Pathway ID Description
sce01200 Carbon metabolism
sce00020 Citrate cycle (TCA cycle)
sce00010 Glycolysis / Gluconeogenesis

Domain Information

InterPro Annotations

Accession Description
IPR001017 Dehydrogenase, E1 component
IPR017597 Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup y
IPR029061 Thiamin diphosphate-binding fold

UniProt Annotations

Entry Information

Gene Name
pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
Protein Entry
ODPA_YEAST
UniProt ID
P16387
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO(2).
Cofactor Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
Enzyme Regulation E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).
Miscellaneous Present with 100000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Ptm Phosphorylated at Ser-313 by pyruvate dehydrogenase kinases PKP1 (PDK1) and PKP2 (PDK2), and dephosphorylated by pyruvate dehydrogenase phosphatases PTC5 and PTC6. {ECO:0000269|PubMed:15665377, ECO:0000269|PubMed:17330950, ECO:0000269|PubMed:17761666, ECO:0000269|PubMed:18180296, ECO:0000269|PubMed:18407956, ECO:0000269|PubMed:19779198}.
Sequence Caution Sequence=AAB64705.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Subcellular Location Mitochondrion matrix.
Subunit Pyruvate dehydrogenase (E1) is a tetramer of 2 alpha and 2 beta subunits. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.

Identical and Related Proteins

Unique RefSeq proteins for LMP006945 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398365125 RefSeq NP_011105 420 pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha

Identical Sequences to LMP006945 proteins

Reference Database Accession Length Protein Name
GI:398365125 GenBank AGP21937.1 420 Sequence 70 from patent US 8465964
GI:398365125 GenBank EWG86679.1 420 Pda1p [Saccharomyces cerevisiae R008]
GI:398365125 GenBank EWG91566.1 420 Pda1p [Saccharomyces cerevisiae P301]
GI:398365125 GenBank EWH18918.1 420 Pda1p [Saccharomyces cerevisiae P283]
GI:398365125 GenBank AIB02136.1 420 Sequence 83 from patent US 8669094
GI:398365125 gnl McCuskerlabDuke 420 Pda1p [Saccharomyces cerevisiae YJM993]

Related Sequences to LMP006945 proteins

Reference Database Accession Length Protein Name
GI:398365125 GenBank AAB64705.1 443 Pda1p: alpha subunit of pyruvate dehydrogenase [Saccharomyces cerevisiae]
GI:398365125 GenBank ABZ27843.1 443 Sequence 1781 from patent US 7314974
GI:398365125 GenBank EDZ72479.1 443 YER178Wp-like protein [Saccharomyces cerevisiae AWRI1631]
GI:398365125 GenBank EGA75216.1 443 Pda1p [Saccharomyces cerevisiae AWRI796]
GI:398365125 GenBank EGA79107.1 443 Pda1p [Saccharomyces cerevisiae Vin13]
GI:398365125 GenBank EHN07455.1 443 Pda1p [Saccharomyces cerevisiae x Saccharomyces kudriavzevii VIN7]