Gene/Proteome Database (LMPD)

LMPD ID
LMP006931
Gene ID
852519
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
pyruvate carboxylase 2
Gene Symbol
PYC2
Synonyms
-
Alternate Names
pyruvate carboxylase 2
Chromosome
II
EC Number
6.4.1.1

Proteins

pyruvate carboxylase 2
Refseq ID NP_009777
Protein GI 6319695
UniProt ID P32327
mRNA ID NM_001178566
Length 1180
RefSeq Status PROVISIONAL
MSSSKKLAGLRDNFSLLGEKNKILVANRGEIPIRIFRSAHELSMRTIAIYSHEDRLSMHRLKADEAYVIGEEGQYTPVGAYLAMDEIIEIAKKHKVDFIHPGYGFLSENSEFADKVVKAGITWIGPPAEVIDSVGDKVSARHLAARANVPTVPGTPGPIETVQEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCFVERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAILTDAVKLAKVCGYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVSAQIQIAAGATLTQLGLLQDKITTRGFSIQCRITTEDPSKNFQPDTGRLEVYRSAGGNGVRLDGGNAYAGATISPHYDSMLVKCSCSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLLTNPVFIEGTYWTTFIDDTPQLFQMVSSQNRAQKLLHYLADLAVNGSSIKGQIGLPKLKSNPSVPHLHDAQGNVINVTKSAPPSGWRQVLLEKGPSEFAKQVRQFNGTLLMDTTWRDAHQSLLATRVRTHDLATIAPTTAHALAGAFALECWGGATFDVAMRFLHEDPWERLRKLRSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLKVGVNAVKKAGGVVEATVCYSGDMLQPGKKYNLDYYLEVVEKIVQMGTHILGIKDMAGTMKPAAAKLLIGSLRTRYPDLPIHVHSHDSAGTAVASMTACALAGADVVDVAINSMSGLTSQPSINALLASLEGNIDTGINVEHVRELDAYWAEMRLLYSCFEADLKGPDPEVYQHEIPGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVSNKLTSDDIRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPLRSDVLRNKRRKLTCRPGLELEPFDLEKIREDLQNRFGDIDECDVASYNMYPRVYEDFQKIRETYGDLSVLPTKNFLAPAEPDEEIEVTIEQGKTLIIKLQAVGDLNKKTGQREVYFELNGELRKIRVADKSQNIQSVAKPKADVHDTHQIGAPMAGVIIEVKVHKGSLVKKGESIAVLSAMKMEMVVSSPADGQVKDVFIKDGESVDASDLLVVLEEETLPPSQKK

Gene Information

Entrez Gene ID
852519
Gene Name
pyruvate carboxylase 2
Gene Symbol
PYC2
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:SGD C cytosol
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003677 IEA:InterPro F DNA binding
GO:0004075 IEA:InterPro F biotin carboxylase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004736 IMP:SGD F pyruvate carboxylase activity
GO:0006094 IMP:SGD P gluconeogenesis

KEGG Pathway Links

KEGG Pathway ID Description
sce01200 Carbon metabolism
sce00020 Citrate cycle (TCA cycle)

BIOCYC Pathway Links

BIOCYC Pathway ID Description
TCA-EUK-PWY-YEAST TCA cycle, aerobic respiration

Domain Information

InterPro Annotations

Accession Description
IPR011761 ATP-grasp fold
IPR013815 ATP-grasp fold, subdomain 1
IPR013816 ATP-grasp fold, subdomain 2
IPR013785 Aldolase-type TIM barrel
IPR005482 Biotin carboxylase, C-terminal
IPR011764 Biotin carboxylation domain
IPR001882 Biotin-binding site
IPR000089 Biotin/lipoyl attachment
IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal
IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
IPR003379 Carboxylase, conserved domain
IPR009057 Homeodomain-like
IPR016185 Pre-ATP-grasp domain
IPR005930 Pyruvate carboxylase
IPR000891 Pyruvate carboxyltransferase
IPR011054 Rudiment single hybrid motif
IPR011053 Single hybrid motif

UniProt Annotations

Entry Information

Gene Name
pyruvate carboxylase 2
Protein Entry
PYC2_YEAST
UniProt ID
P32327
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate.
Cofactor Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Cofactor Name=biotin; Xref=ChEBI:CHEBI:57586; Note=Biotin.;
Function Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Induction By glucose.
Miscellaneous Present with 17000 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Pathway Carbohydrate biosynthesis; gluconeogenesis.
Similarity Contains 1 ATP-grasp domain. {ECO:0000255|PROSITE- ProRule:PRU00409}.
Similarity Contains 1 biotin carboxylation domain. {ECO:0000305}.
Similarity Contains 1 biotinyl-binding domain. {ECO:0000255|PROSITE-ProRule:PRU01066, ECO:0000305}.
Similarity Contains 1 carboxyltransferase domain. {ECO:0000305}.
Subcellular Location Cytoplasm.
Subunit Homotetramer.

Identical and Related Proteins

Unique RefSeq proteins for LMP006931 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6319695 RefSeq NP_009777 1180 pyruvate carboxylase 2

Identical Sequences to LMP006931 proteins

Reference Database Accession Length Protein Name
GI:6319695 GenBank AGX52170.1 1180 Sequence 4695 from patent US 8541208
GI:6319695 GenBank AGX52802.1 1180 Sequence 5955 from patent US 8541208
GI:6319695 GenBank AGX53738.1 1180 Sequence 7826 from patent US 8541208
GI:6319695 GenBank AGX56005.1 1180 Sequence 12336 from patent US 8541208
GI:6319695 GenBank AGX60420.1 1180 Sequence 21071 from patent US 8541208
GI:6319695 GenBank AGX77053.1 1180 Sequence 53880 from patent US 8541208

Related Sequences to LMP006931 proteins

Reference Database Accession Length Protein Name
GI:6319695 DBBJ GAA21764.1 1180 K7_Pyc2p [Saccharomyces cerevisiae Kyokai no. 7]
GI:6319695 GenBank EDN64827.1 1180 pyruvate carboxylase [Saccharomyces cerevisiae YJM789]
GI:6319695 GenBank EDV11895.1 1180 pyruvate carboxylase [Saccharomyces cerevisiae RM11-1a]
GI:6319695 GenBank EWG87784.1 1180 Pyc2p [Saccharomyces cerevisiae R008]
GI:6319695 GenBank EWG92550.1 1191 Pyc2p [Saccharomyces cerevisiae P301]
GI:6319695 gnl McCuskerlabDuke 1180 Pyc2p [Saccharomyces cerevisiae YJM993]