LIPID MAPS

LMPD ID

LMP006853

Gene ID

850574

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

1-phosphatidylinositol-3-phosphate 5-kinase

Gene Symbol

FAB1

Synonyms

SVL7

Chromosome

VI

EC Number

2.7.1.150

1-phosphatidylinositol-3-phosphate 5-kinase
Refseq ID	NP_116674
Protein GI	330443539
UniProt ID	P34756
mRNA ID	NM_001179984
Length	2278
MSSEEPHASISFPDGSHVRSSSTGTSSVNTIDATLSRPNYIKKPSLHIMSTSTTSTTTDLVTNPILSNISVPKISPPTSSSIATATSTSHVTGTASHSNIKANANTSTSVNKKNLPPTTSGRIPSSTIKRYPSRYKPSHSLQLPIKNDSNFKRSSIYASKSTVTAIPIRNNRPISMQNSYARTPDSDHDDVGDEVSSIKSASSSLTASLSKSFLFAFYNNRKKDKTSNNGVLSKEYWMKDESSKECFSCGKTFNTFRRKHHCRICGQIFCSSCTLLIDGDRFGCHAKMRVCYNCYEHADTYEDSSDEENDSTMQLNEPRSRSRSRSSNTNPYSHSHSHLHLISQDNHNGTDLHDPVAATDNPQQQNEVYLLNDDDVQSIMTSGEDSKLFISTPPPPPKMAIPATKQGGSLEISFDSENDRALHYQDDNPGRHHHLDSVPTRYTIRDMDNISHYDTNSNSTLRPHYNTNNSTITINNLNNTTSNNSNYNNTNSNSNINNPAHSLRRSIFHYVSSNSVNKDSNNSSATPASSAQSSSILDPANRIIGNYAHRNYKFKFNYNSKGPSQQNDTANGNNDNNNNNNNNNNNNNNNSASGIADNNNIPSNDNGTTFTLDKKKRNPLTKSKSTSAYLEYPLNEEDSSEDEGSMSIYSVLNDDHKTDNPIRSMRNSTKSFQRAQASLQRMRFRRKSKSKHFPNNSKSSIYRDLNFLTNSTPNLLSVVSDDNLYDDSSPLQDKASSSAASRLTDRKFSNSSGSNNNSNSNSNINTDPWKRIASISGFKLKKEKKRELNEVSLLHMHALLKQLLNDQEISNLQEWITLLDGALRKVLRTILNARDLNTLDFRQTYVKIKRISGGSPQNSEYIDGVVFSKALPSKTMPRHLKNPRILLIMFPLEYQKNNNHFLSIESVFRQEREYLDKLVSRLKSLHPDIIYVGANVSGYALELLNDSGIVVQFNMKPQVIERIAKLTEADIAISVDKLATNIKMGECETFEVKSYIYGNISKTYTFLRGCNPELGGTILLRGDSLENLRKIKQVSEFMVYAIFSLKLESSFFNDNFIQLSTDVYLKRAESKKLQVFEGYFADFLIKFNNRILTVSPTVDFPIPFLLEKARGLEKKLIERINQYESESDLDRQTQLNMLQGLESTITKKHLGNLIKFLHEMEIENLELEFQKRSRQWEVSYSSSQNLLGTGSHQSITVLYSMVSTKTATPCVGPQIVTIDYFWDSDISIGQFIENVVGTARYPCQQGCNGLYLDHYRSYVHGSGKVDVLIEKFQTRLPKLKDIILTWSYCKKCGTSTPILQISEKTWNHSFGKYLEVMFWSYKDSVTGIGKCPHDFTKDHVKYFGYNDLVVRLEYSDLEVHELITPPRKIKWKPHIDIKLKVELYYKILEKINNFYGSVLSRLERIKLDSMTKDKVLSGQAKIIELKSNATEEQKLMLQDLDTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIFAKSYLPSETDISRITAKQLKKLFYDSSRKDSEDKKSLHDEKAKTRKPEKNELPLEGLKDVEKPKIDSKNTTENRDRTNEPQNAVTITTFKDDTPIIPTSGTSHLTVTPSASSVSSSLTPQTEERPPISRSGTGISMTHDKSTRPNIRKMSSDSSLCGLASLANEYSKNNKVSKLATFFDQMHFDALSKEFELERERERLQLNKDKYQAIRLQTSTPIVEIYKNVKDAVDEPLHSRSSGNNLSSANVKTLEAPVGEHSRANNCNPPNLDQNLETELENSISQWGENILNPSGKTTASTHLNSKPVVKETSENPKSIVRESDNSKSEPLPPVITTTTVNKVESTPQPEKSLLMKTLSNFWADRSAYLWKPLVYPTCPSEHIFTDSDVIIREDEPSSLIAFCLSTSDYRNKMMNLNVQQQQQQQTAEAAPAKTGGNSGGTTQTGDPSVNISPSVSTTSHNKGRDSEISSLVTTKEGLLNTPPIEGARDRTPQESQTHSQANLDTLQELEKIMTKKTATHLRYQFEEGLTVMSCKIFFTEHFDVFRKICDCQENFIQSLSRCVKWDSNGGKSGSGFLKTLDDRFIIKELSHAELEAFIKFAPSYFEYMAQAMFHDLPTTLAKVFGFYQIQVKSSISSSKSYKMDVIIMENLFYEKKTTRIFDLKGSMRNRHVEQTGKANEVLLDENMVEYIYESPIHVREYDKKLLRASVWNDTLFLAKMNVMDYSLVIGIDNEGYTLTVGIIDFIRTFTWDKKLESWVKEKGLVGGASVIKQPTVVTPRQYKKRFREAMERYILMVPDPWYREGN

Entrez Gene ID

850574

Gene Name

1-phosphatidylinositol-3-phosphate 5-kinase

Gene Symbol

FAB1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0070772	IDA:SGD	C	PAS complex
GO:0010008	IDA:SGD	C	endosome membrane
GO:0000329	IDA:SGD	C	fungal-type vacuole membrane
GO:0000285	IDA:SGD	F	1-phosphatidylinositol-3-phosphate 5-kinase activity
GO:0005524	IEA:UniProtKB-KW	F	ATP binding
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0032266	IDA:SGD	F	phosphatidylinositol-3-phosphate binding
GO:0044267	IEA:InterPro	P	cellular protein metabolic process
GO:0046854	IDA:SGD	P	phosphatidylinositol phosphorylation

KEGG Pathway Links

KEGG Pathway ID	Description
ko00562	Inositol phosphate metabolism
sce00562	Inositol phosphate metabolism
ko04145	Phagosome
sce04145	Phagosome
ko04070	Phosphatidylinositol signaling system
sce04070	Phosphatidylinositol signaling system

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-6352	3-phosphoinositide biosynthesis
PWY3O-242	phosphatidylinositol phosphate biosynthesis
PWY-6371	superpathway of inositol phosphate compounds

REACTOME Pathway Links

REACTOME Pathway ID	Description
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618611	PI Metabolism
5618126	Phospholipid metabolism
5618614	Synthesis of PIPs at the Golgi membrane
5618615	Synthesis of PIPs at the early endosome membrane
5618612	Synthesis of PIPs at the late endosome membrane

InterPro Annotations

Accession	Description
IPR002423	Chaperonin Cpn60/TCP-1
IPR000306	FYVE zinc finger
IPR027409	GroEL-like apical domain
IPR027483	Phosphatidylinositol-4-phosphate 5-kinase, C-terminal
IPR027484	Phosphatidylinositol-4-phosphate 5-kinase, N-terminal domain
IPR002498	Phosphatidylinositol-4-phosphate 5-kinase, core
IPR016034	Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup
IPR017455	Zinc finger, FYVE-related
IPR011011	Zinc finger, FYVE/PHD-type
IPR013083	Zinc finger, RING/FYVE/PHD-type

Entry Information

Gene Name

1-phosphatidylinositol-3-phosphate 5-kinase

Protein Entry

FAB1_YEAST

UniProt ID

P34756

Species

Yeast (S288c)

Comments

Comment Type	Description
Catalytic Activity	ATP + 1-phosphatidyl-1D-myo-inositol 3- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
Catalytic Activity	ATP + 1-phosphatidyl-1D-myo-inositol 3- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate. {ECO:0000269\|PubMed:9811604}.
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
Enzyme Regulation	Activated by VAC14 and VAC7. VAC14 acts as a specific osmotic response regulator. {ECO:0000269\|PubMed:11889142, ECO:0000269\|PubMed:11950935}.
Function	The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. The product of the reaction it catalyzes functions as an important regulator of vacuole homeostasis perhaps by controlling membrane flux to and/or from the vacuole. Hyperosmotic shock-induced increase in the levels of PtdIns(3,5)P2 requires the presence of VAC7, VAC14, and/or FIG4. {ECO:0000269\|PubMed:16492811, ECO:0000269\|PubMed:9811604}.
Interaction	P42837:FIG4; NbExp=4; IntAct=EBI-6754, EBI-28407; Q06708:VAC14; NbExp=9; IntAct=EBI-6754, EBI-27189;
Miscellaneous	Present with 149 molecules/cell in log phase SD medium
Miscellaneous	Present with 149 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Contains 1 FYVE-type zinc finger
Similarity	Contains 1 FYVE-type zinc finger. {ECO:0000255\|PROSITE-ProRule:PRU00091}.
Similarity	Contains 1 PIPK domain. {ECO:0000255\|PROSITE- ProRule:PRU00781}.
Subcellular Location	Vacuole membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Localization to the vacuole membrane requires VAC14 and FIG4.
Subunit	Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold
Subunit	Component of the PI(3,5)P2 regulatory complex, composed of ATG18, FIG4, FAB1, VAC14 and VAC7. VAC14 nucleates the assembly of the complex and serves as a scaffold. {ECO:0000269\|PubMed:18653468, ECO:0000269\|PubMed:19037259}.