LIPID MAPS

LMPD ID

LMP006838

Gene ID

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

dihydroxy-acid dehydratase ILV3

Gene Symbol

ILV3

Synonyms

-

Chromosome

X

EC Number

4.2.1.9

dihydroxy-acid dehydratase ILV3
Refseq ID	NP_012550
Protein GI	6322476
UniProt ID	P39522
mRNA ID	NM_001181674
Length	585
MGLLTKVATSRQFSTTRCVAKKLNKYSYIITEPKGQGASQAMLYATGFKKEDFKKPQVGVGSCWWSGNPCNMHLLDLNNRCSQSIEKAGLKAMQFNTIGVSDGISMGTKGMRYSLQSREIIADSFETIMMAQHYDANIAIPSCDKNMPGVMMAMGRHNRPSIMVYGGTILPGHPTCGSSKISKNIDIVSAFQSYGEYISKQFTEEEREDVVEHACPGPGSCGGMYTANTMASAAEVLGLTIPNSSSFPAVSKEKLAECDNIGEYIKKTMELGILPRDILTKEAFENAITYVVATGGSTNAVLHLVAVAHSAGVKLSPDDFQRISDTTPLIGDFKPSGKYVMADLINVGGTQSVIKYLYENNMLHGNTMTVTGDTLAERAKKAPSLPEGQEIIKPLSHPIKANGHLQILYGSLAPGGAVGKITGKEGTYFKGRARVFEEEGAFIEALERGEIKKGEKTVVVIRYEGPRGAPGMPEMLKPSSALMGYGLGKDVALLTDGRFSGGSHGFLIGHIVPEAAEGGPIGLVRDGDEIIIDADNNKIDLLVSDKEMAQRKQSWVAPPPRYTRGTLSKYAKLVSNASNGCVLDA

Entrez Gene ID

Gene Name

dihydroxy-acid dehydratase ILV3

Gene Symbol

ILV3

Species

Saccharomyces cerevisiae S288c

GO ID	Source	Type	Description
GO:0005739	IEA:UniProtKB-KW	C	mitochondrion
GO:0051539	IEA:UniProtKB-KW	F	4 iron, 4 sulfur cluster binding
GO:0004160	IMP:SGD	F	dihydroxy-acid dehydratase activity
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0009082	IMP:SGD	P	branched-chain amino acid biosynthetic process
GO:0009097	IEA:UniProtKB-UniPathway	P	isoleucine biosynthetic process
GO:0009099	IEA:UniProtKB-UniPathway	P	valine biosynthetic process

KEGG Pathway ID	Description
ko01210	2-Oxocarboxylic acid metabolism
sce01210	2-Oxocarboxylic acid metabolism
ko01230	Biosynthesis of amino acids
sce01230	Biosynthesis of amino acids
sce01110	Biosynthesis of secondary metabolites
M00570	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
sce_M00570	Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
sce01100	Metabolic pathways
ko00770	Pantothenate and CoA biosynthesis
sce00770	Pantothenate and CoA biosynthesis
ko00290	Valine, leucine and isoleucine biosynthesis
sce00290	Valine, leucine and isoleucine biosynthesis
M00019	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine
sce_M00019	Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine

BIOCYC Pathway ID	Description
ILEUSYN-PWY	isoleucine biosynthesis
BRANCHED-CHAIN-AA-SYN-PWY	superpathway of leucine, valine, and isoleucine biosynthesis
VALSYN-PWY	valine biosynthesis

Accession	Description
IPR015928	Aconitase/3-isopropylmalate dehydratase, swivel
IPR000581	DiOHA_6PGluconate_deHydtase
IPR020558	DiOHA_6PGluconate_deHydtase_CS
IPR004404	DihydroxyA_deHydtase

Gene Name

dihydroxy-acid dehydratase ILV3

Protein Entry

ILV3_YEAST

UniProt ID

Species

Yeast (S288c)

Comment Type	Description
Catalytic Activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2- oxobutanoate + H(2)O.
Cofactor	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000305};
Cofactor	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence= ; Note=Binds 1 [4Fe-4S] cluster. ;
Miscellaneous	Present with 171000 molecules/cell in log phase SD medium
Miscellaneous	Present with 171000 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L- isoleucine from 2-oxobutanoate: step 3/4.
Pathway	Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.
Similarity	Belongs to the IlvD/Edd family
Similarity	Belongs to the IlvD/Edd family. {ECO:0000305}.
Subcellular Location	Mitochondrion.
Subunit	Homodimer.