LIPID MAPS

LMPD ID

LMP006825

Gene ID

855983

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Vps30p

Gene Symbol

VPS30

Synonyms

APG6; ATG6; VPT30

Chromosome

XVI

Vps30p
Refseq ID	NP_015205
Protein GI	6325137
UniProt ID	Q02948
mRNA ID	NM_001183934
Length	557
MKCQTCHLPLQLDPSLEGLSLTQRNLLLSNNSIITATNENVISNKGIEAADNCGPQIPKERLRRLGEIQNIKDLNLKDDKLITDSFVFLNHDDDDNANITSNSREDQRYGNANGNDNKKANSDTSDGTSTFRDHDEEEQEATDEDENQQIQLNSKTLSTQVNAMTNVFNILSSQTNIDFPICQDCCNILINRLKSEYDDAIKERDTYAQFLSKLESQNKEISESNKEKQYSHNLSEKENLKKEEERLLDQLLRLEMTDDDLDGELVRLQEKKVQLENEKLQKLSDQNLMDLNNIQFNKNLQSLKLQYELSLNQLDKLRKINIFNATFKISHSGPFATINGLRLGSIPESVVPWKEINAALGQLILLLATINKNLKINLVDYELQPMGSFSKIKKRMVNSVEYNNSTTNAPGDWLILPVYYDENFNLGRIFRKETKFDKSLETTLEIISEITRQLSTIASSYSSQTLTTSQDESSMNNANDVENSTSILELPYIMNKDKINGLSVKLHGSSPNLEWTTAMKFLLTNVKWLLAFSSNLLSKSITLSPTVNYNDKTISGN

Entrez Gene ID

855983

Gene Name

Vps30p

Gene Symbol

VPS30

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005737	IDA:SGD	C	cytoplasm
GO:0005768	IEA:UniProtKB-KW	C	endosome
GO:0019898	IDA:SGD	C	extrinsic component of membrane
GO:0034271	IDA:SGD	C	phosphatidylinositol 3-kinase complex, class III, type I
GO:0034272	IDA:SGD	C	phosphatidylinositol 3-kinase complex, class III, type II
GO:0005773	IEA:UniProtKB-KW	C	vacuole
GO:0032258	IMP:SGD	P	CVT pathway
GO:0006914	IMP:SGD	P	autophagy
GO:0045324	IMP:SGD	P	late endosome to vacuole transport
GO:0016236	IMP:SGD	P	macroautophagy
GO:0030242	IMP:SGD	P	peroxisome degradation
GO:0006661	IMP:SGD	P	phosphatidylinositol biosynthetic process
GO:0034727	IMP:SGD	P	piecemeal microautophagy of nucleus
GO:0006623	IDA:UniProtKB	P	protein targeting to vacuole
GO:0042147	IMP:SGD	P	retrograde transport, endosome to Golgi

KEGG Pathway Links

KEGG Pathway ID	Description
ko04140	Regulation of autophagy
sce04140	Regulation of autophagy

InterPro Annotations

Accession	Description
IPR007243	Atg6/Beclin

Entry Information

Gene Name

Vps30p

Protein Entry

BECN1_YEAST

UniProt ID

Q02948

Species

Yeast (S288c)

Comments

Comment Type	Description
Domain	The C-terminal domain called the BARA domain is dispensable for the construction of both VPS34 PI3-kinase complexes, but is specifically required for autophagy through the targeting of complex I to the pre-autophagosomal structure
Domain	The C-terminal domain called the BARA domain is dispensable for the construction of both VPS34 PI3-kinase complexes, but is specifically required for autophagy through the targeting of complex I to the pre-autophagosomal structure. {ECO:0000269\|PubMed:22437838}.
Function	Required for cytoplasm to vacuole transport (Cvt), autophagy, nucleophagy, and mitophagy, as a part of the autophagy- specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3- kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Plays also a role in regulation of filamentous growth. {ECO:0000269\|PubMed:11157979, ECO:0000269\|PubMed:11689437, ECO:0000269\|PubMed:12244127, ECO:0000269\|PubMed:16267277, ECO:0000269\|PubMed:17404498, ECO:0000269\|PubMed:17700056, ECO:0000269\|PubMed:18182384, ECO:0000269\|PubMed:18701704, ECO:0000269\|PubMed:19131141, ECO:0000269\|PubMed:22437838, ECO:0000269\|PubMed:8224160, ECO:0000269\|PubMed:9105038, ECO:0000269\|PubMed:9712845}.
Interaction	P38270:ATG14; NbExp=7; IntAct=EBI-2670, EBI-2699; Q05919:VPS38; NbExp=8; IntAct=EBI-2670, EBI-29972;
Miscellaneous	Present with 2490 molecules/cell in log phase SD medium
Miscellaneous	Present with 2490 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity	Belongs to the beclin family
Similarity	Belongs to the beclin family. {ECO:0000305}.
Subcellular Location	Endosome membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. Note=With the VPS34 PI3-kinase complex I, localizes to the vacuole-isolation membrane contact site (VICS) during isolation membrane (IM) expansion. The IM is a membrane sac generated from the pre-autophagosomal structure that ultimately expands to become a mature autophagosome.
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38
Subunit	Component of the autophagy-specific VPS34 PI3-kinase complex I composed of VPS15, VPS30, VPS34, ATG14 and ATG38; and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. {ECO:0000269\|PubMed:24165940}.