LIPID MAPS

LMPD ID

LMP006815

Gene ID

855593

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

diacylglycerol cholinephosphotransferase

Gene Symbol

CPT1

Synonyms

-

Alternate Names

diacylglycerol cholinephosphotransferase

Chromosome

XIV

EC Number

2.7.8.2

diacylglycerol cholinephosphotransferase
Refseq ID	NP_014269
Protein GI	398364723
UniProt ID	P17898
mRNA ID	NM_001182968
Length	393
RefSeq Status	PROVISIONAL
MGFFIPQSSLGNLKLYKYQSDDRSFLSNHVLRPFWRKFATIFPLWMAPNLVTLLGFCFIIFNVLTTLYYDPYFDQESPRWTYFSYAIGLFLYQTFDACDGMHARRTGQQGPLGELFDHCIDSINTTLSMIPVCSMTGMGYTYMTIFSQFAILCSFYLSTWEEYHTHKLYLAEFCGPVEGIIVLCISFIAVGIYGPQTIWHTKVAQFSWQDFVFDVETVHLMYAFCTGALIFNIVTAHTNVVRYYESQSTKSATPSKTAENISKAVNGLLPFFAYFSSIFTLVLIQPSFISLALILSIGFSVAFVVGRMIIAHLTMQPFPMVNFPFLIPTIQLVLYAFMVYVLDYQKGSIVSALVWMGLGLTLAIHGMFINDIIYDITTFLDIYALSIKHPKEI

Entrez Gene ID

855593

Gene Name

diacylglycerol cholinephosphotransferase

Gene Symbol

CPT1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005794	IDA:SGD	C	Golgi apparatus
GO:0005783	IEA:UniProtKB-KW	C	endoplasmic reticulum
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0005741	IDA:SGD	C	mitochondrial outer membrane
GO:0004142	IDA:SGD	F	diacylglycerol cholinephosphotransferase activity
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0006657	IDA:SGD	P	CDP-choline pathway

KEGG Pathway Links

KEGG Pathway ID	Description
sce_M00090	Phosphatidylcholine (PC) biosynthesis, choline => PC

BIOCYC Pathway Links

BIOCYC Pathway ID	Description
PWY-7367	phosphatidylcholine resynthesis via glycerophosphocholine

InterPro Annotations

Accession	Description
IPR000462	CDP-alcohol phosphatidyltransferase
IPR014472	Choline/ethanolamine phosphotransferase

Entry Information

Gene Name

diacylglycerol cholinephosphotransferase

Protein Entry

CPT1_YEAST

UniProt ID

P17898

Species

Yeast (S288c)

Comments

Comment Type	Description
Biophysicochemical Properties	Kinetic parameters: KM=100 uM for CDP-choline {ECO:0000269\|PubMed:1847919}; KM=137 uM for CDP-dimethylethanolamine {ECO:0000269\|PubMed:1847919}; Vmax=0.2 nmol/min/mg enzyme for CDP-choline {ECO:0000269\|PubMed:1847919}; Vmax=0.07 nmol/min/mg enzyme for CDP-dimethylethanolamine {ECO:0000269\|PubMed:1847919};
Catalytic Activity	CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine. {ECO:0000269\|PubMed:15225629, ECO:0000269\|PubMed:1847919}.
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269\|PubMed:1847919};
Enzyme Regulation	Requires a divalent cation activator, and is inhibited by CMP. Activated by phospholipids, especially phosphatidylcholine. {ECO:0000269\|PubMed:1847919}.
Function	Catalyzes the final step in the CDP-choline route leading to phosphatidylcholin (PC). Preferentially uses CDP- monomethylethanolamine as aminoalcohol substrate. Shows highest activity toward di- and mono-unsaturated diacylglycerol species as lipid substrates. The CDP-choline pathway only contributes to net PC synthesis if exogenous choline is present. In its absence, this pathway recycles choline from PC turnover and may contribute to maintaining the proper PC species composition. {ECO:0000269\|PubMed:15225629, ECO:0000269\|PubMed:1847919, ECO:0000269\|PubMed:3029130, ECO:0000269\|PubMed:7961445, ECO:0000269\|PubMed:7961735}.
Induction	Repressed by inositol. {ECO:0000269\|PubMed:7961831}.
Miscellaneous	Present with 981 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.
Sequence Caution	Sequence=AAA63571.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA86895.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAA96012.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity	Belongs to the CDP-alcohol phosphatidyltransferase class-I family. {ECO:0000305}.
Subcellular Location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein.

Unique RefSeq proteins for LMP006815 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
398364723	RefSeq	NP_014269	393	diacylglycerol cholinephosphotransferase

Identical Sequences to LMP006815 proteins

Reference	Database	Accession	Length	Protein Name
GI:398364723	GenBank	EWG83714.1	393	Cpt1p [Saccharomyces cerevisiae R008]
GI:398364723	SwissProt	P17898.3	393	RecName: Full=Cholinephosphotransferase 1; AltName: Full=Aminoalcohol phosphotransferase CPT1; AltName: Full=Diacylglycerol cholinephosphotransferase 1; AltName: Full=Sn-1,2-diacylglycerol cholinephosphotransferase; Short=CHOPT [Saccharomyces cerevisiae S288c]
GI:398364723	Third Party Genbank	DAA10418.1	393	TPA: diacylglycerol cholinephosphotransferase [Saccharomyces cerevisiae S288c]

Related Sequences to LMP006815 proteins

Reference	Database	Accession	Length	Protein Name
GI:398364723	EMBL	CAA86895.1	407	sn-1,2-diacylglycerol cholinephosphotransferase [Saccharomyces cerevisiae]
GI:398364723	GenBank	AAA63571.1	407	sn-1,2-diacylglycerol cholinephosphotransferase [Saccharomyces cerevisiae]
GI:398364723	GenBank	EDN62685.1	393	diacylglycerol cholinephosphotransferase [Saccharomyces cerevisiae YJM789]
GI:398364723	GenBank	EDV12382.1	393	sn-1,2-diacylglycerol cholinephosphotransferase [Saccharomyces cerevisiae RM11-1a]
GI:398364723	GenBank	EIW07963.1	407	Cpt1p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:398364723	gnl	McCuskerlabDuke	393	Cpt1p [Saccharomyces cerevisiae YJM993]