Gene/Proteome Database (LMPD)

LMPD ID
LMP004020
Gene ID
96979
Species
Mus musculus (Mouse)
Gene Name
prostaglandin E synthase 2
Gene Symbol
Ptges2
Synonyms
0610038H10Rik; C79137; Gbf1; Mpges2; Pges2
Alternate Names
prostaglandin E synthase 2; mPGES-2; GATE-binding factor 1; microsomal prostaglandin E synthase 2
Chromosome
2
Map Location
2 B|2
EC Number
5.3.99.3
Summary
The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate, which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However, a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein, but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE), possibly via an interaction with CAAAT/enhancer-binding protein-beta. [provided by RefSeq, Oct 2009]
Orthologs
View orthologs and multiple alignments for Ptges2

Proteins

prostaglandin E synthase 2
Refseq ID NP_598544
Protein GI 260763900
UniProt ID Q8BWM0
mRNA ID NM_133783
Length 384
RefSeq Status REVIEWED
MAQAARLSWVLVSSRCALTEGLLTRPWQPLSAQSRAGFTRVAAGSRGAAVRKGSPRLLGAAALALGGALGLYHTVRWHQRSQDLRAERSAAQLPLSNSLQLTLYQYKTCPFCSKVRAFLDFHSLPYQVVEVNPVRRTEIKFSSYRKVPILVAQEGDSLQQLNDSSVIISALKTYLVSGQPLEEVITYYPPMKAMNDQGKEVTEFCNKYWLMLDEKEAQQMYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVREGKFGAVEAAMAKYVGAAAMYLISKRLKSRHHLQDDVRVDLYEAANKWVTAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLEAFDDLMRHSHIQPWYLRMERAIEEAPSVHHVNPSCKD

Gene Information

Entrez Gene ID
96979
Gene Name
prostaglandin E synthase 2
Gene Symbol
Ptges2
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0005829 IDA:MGI C cytosol
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005739 IDA:MGI C mitochondrion
GO:0005634 IDA:MGI C nucleus
GO:0003677 IDA:MGI F DNA binding
GO:0009055 IEA:InterPro F electron carrier activity
GO:0043295 ISS:UniProtKB F glutathione binding
GO:0020037 ISS:UniProtKB F heme binding
GO:0016829 ISS:UniProtKB F lyase activity
GO:0050220 IEA:UniProtKB-EC F prostaglandin-E synthase activity
GO:0015035 IEA:InterPro F protein disulfide oxidoreductase activity
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0045893 IDA:MGI P positive regulation of transcription, DNA-templated
GO:0001516 IEA:UniProtKB-UniPathway P prostaglandin biosynthetic process
GO:0046903 IDA:MGI P secretion

KEGG Pathway Links

KEGG Pathway ID Description
mmu00590 Arachidonic acid metabolism
mmu01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY3DJ-35583 biosynthesis of prostaglandins

Domain Information

InterPro Annotations

Accession Description
IPR002109 Glutaredoxin
IPR004046 Glutathione S-transferase, C-terminal
IPR010987 Glutathione S-transferase, C-terminal-like
IPR004045 Glutathione S-transferase, N-terminal
IPR012336 Thioredoxin-like fold

UniProt Annotations

Entry Information

Gene Name
prostaglandin E synthase 2
Protein Entry
PGES2_MOUSE
UniProt ID
Q8BWM0
Species
Mouse

Comments

Comment Type Description
Catalytic Activity (5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15- hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15- dihydroxy-9-oxoprosta-5,13-dienoate. {ECO:0000250|UniProtKB:Q9H7Z7}.
Enzyme Regulation Isomerase activity is increased by sulfhydril compounds. Dithiothreitol (DTT) is most effective, followed by glutathione (GSH) and 2-mercaptoethanol. {ECO:0000250|UniProtKB:Q66LN0}.
Function Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. {ECO:0000250|UniProtKB:Q9H7Z7, ECO:0000303|PubMed:12050152}.
Induction Constitutively expressed. Not induced during tissue inflammation. Down-regulated in the absence of Ptges. {ECO:0000269|PubMed:12835322, ECO:0000269|PubMed:15584915}.
Pathway Lipid metabolism; prostaglandin biosynthesis.
Similarity Belongs to the GST superfamily. {ECO:0000305}.
Similarity Contains 1 GST C-terminal domain. {ECO:0000305}.
Similarity Contains 1 glutaredoxin domain. {ECO:0000255|PROSITE- ProRule:PRU00686}.
Subcellular Location Golgi apparatus membrane {ECO:0000250|UniProtKB:Q9H7Z7}; Single-pass membrane protein {ECO:0000250|UniProtKB:Q9H7Z7}. Nucleus {ECO:0000269|PubMed:12050152}. Note=According to PubMed:12050152, some fraction may be nuclear. {ECO:0000269|PubMed:12050152}.
Subcellular Location Prostaglandin E synthase 2 truncated form: Cytoplasm. Note=Synthesized as a Golgi membrane-bound protein, which is further cleaved into the predominant soluble truncated form.
Subunit Homodimer. Interacts with EXOSC10 (By similarity). May interact with CEBPB. {ECO:0000250|UniProtKB:Q66LN0, ECO:0000250|UniProtKB:Q9H7Z7, ECO:0000269|PubMed:15879117}.
Tissue Specificity Widely expressed. Expressed in brain, heart, liver, colon and lung. {ECO:0000269|PubMed:12050152, ECO:0000269|PubMed:12835322}.

Identical and Related Proteins

Unique RefSeq proteins for LMP004020 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
260763900 RefSeq NP_598544 384 prostaglandin E synthase 2

Identical Sequences to LMP004020 proteins

Reference Database Accession Length Protein Name
GI:260763900 DBBJ BAC34345.1 384 unnamed protein product [Mus musculus]
GI:260763900 GenBank EDL08547.1 384 prostaglandin E synthase 2, isoform CRA_b [Mus musculus]
GI:260763900 SwissProt Q8BWM0.3 384 RecName: Full=Prostaglandin E synthase 2; AltName: Full=GATE-binding factor 1; Short=GBF-1; AltName: Full=Microsomal prostaglandin E synthase 2; Short=mPGES-2; Contains: RecName: Full=Prostaglandin E synthase 2 truncated form [Mus musculus]

Related Sequences to LMP004020 proteins

Reference Database Accession Length Protein Name
GI:260763900 GenBank AAH04846.1 384 Prostaglandin E synthase 2 [Mus musculus]
GI:260763900 GenBank EDL93235.1 384 prostaglandin E synthase 2 (predicted), isoform CRA_b [Rattus norvegicus]
GI:260763900 GenBank AEU43486.1 384 Sequence 235 from patent US 8052970
GI:260763900 RefSeq NP_001101302.1 384 prostaglandin E synthase 2 [Rattus norvegicus]
GI:260763900 RefSeq XP_005087735.1 382 PREDICTED: prostaglandin E synthase 2 [Mesocricetus auratus]
GI:260763900 RefSeq XP_005372515.1 393 PREDICTED: LOW QUALITY PROTEIN: prostaglandin E synthase 2 [Microtus ochrogaster]