LIPID MAPS

LMPD ID

LMP003243

Gene ID

233081

Species

Mus musculus (Mouse)

Gene Name

free fatty acid receptor 1

Gene Symbol

Ffar1

Synonyms

Gpr40

Alternate Names

free fatty acid receptor 1; G protein-coupled receptor 40; G-protein coupled receptor 40; G protein-coupled receptor GPR40

Chromosome

7

Map Location

7 B1|7

free fatty acid receptor 1
Refseq ID	NP_918946
Protein GI	110835701
UniProt ID	Q76JU9
mRNA ID	NM_194057
Length	300
RefSeq Status	PROVISIONAL
MDLPPQLSFALYVSAFALGFPLNLLAIRGAVSHAKLRLTPSLVYTLHLGCSDLLLAITLPLKAVEALASGAWPLPLPFCPVFALAHFAPLYAGGGFLAALSAGRYLGAAFPFGYQAIRRPRYSWGVCVAIWALVLCHLGLALGLETSGSWLDNSTSSLGINIPVNGSPVCLEAWDPDSARPARLSFSILLFFLPLVITAFCYVGCLRALVRSGLSHKRKLRAAWVAGGALLTLLLCLGPYNASNVASFINPDLGGSWRKLGLITGAWSVVLNPLVTGYLGTGPGRGTICVTRTQRGTIQK

Entrez Gene ID

233081

Gene Name

free fatty acid receptor 1

Gene Symbol

Ffar1

Species

Mus musculus

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0005886	IEA:UniProtKB-KW	C	plasma membrane
GO:0004930	IEA:UniProtKB-KW	F	G-protein coupled receptor activity
GO:0008289	IEA:UniProtKB-KW	F	lipid binding
GO:0042593	IMP:MGI	P	glucose homeostasis
GO:0030073	IMP:MGI	P	insulin secretion

KEGG Pathway Links

KEGG Pathway ID	Description
mmu04911	Insulin secretion

REACTOME Pathway Links

REACTOME Pathway ID	Description
5893729	Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
5893811	Free fatty acid receptors

InterPro Annotations

Accession	Description
IPR013312	G protein-coupled receptor 40-related receptor
IPR000276	G protein-coupled receptor, rhodopsin-like
IPR017452	GPCR, rhodopsin-like, 7TM
IPR013313	GPR40 receptor fatty acid

Entry Information

Gene Name

free fatty acid receptor 1

Protein Entry

FFAR1_MOUSE

UniProt ID

Q76JU9

Species

Mouse

Comments

Comment Type	Description
Disruption Phenotype	No visible phenotype at birth and during the following six weeks. Male mice tend to develop a slightly decreased glucose tolerance after 13 weeks of age, but this is not observed with female mice. Insulin secretion in response to glucose is unchanged in mutant mice, but it is not potentiated by fatty acids, contrary to what is observed with wild-type mice. On the other hand, wild-type and mutant mice display the same inhibition of the first phase of glucose-stimulated insulin secretion after prolonged exposure to fatty acids or exposure to a high-fat diet (PubMed:17395749 and PubMed:18559658). Compared to wild-type, mutant mice that are kept on a high-fat diet display a decrease of the second phase of glucose-stimulated insulin secretion (PubMed:18559658). Mutant mice do not display increased secretion of glucagon-like peptide 1 (GLP-1) in response to oral absorption of corn oil and display slightly increased blood glucose levels after oral absorption of corn oil (PubMed:23403053). Besides, mutant mice display decreased bone density (PubMed:23335512). {ECO:0000269\|PubMed:17395749, ECO:0000269\|PubMed:18559658, ECO:0000269\|PubMed:23335512, ECO:0000269\|PubMed:23403053}.
Enzyme Regulation	Is also activated by synthetic agonists, such as AM-8182, AM-6331 and TAK-875 (fasiglifam). AM-8182 is a full agonist, while AM-6331 and TAK-875 (fasiglifam) are partial agonists that potentiate the activity of the endogenous ligands, such as alpha-linolenic acid and gamma-linolenic acid. {ECO:0000269\|PubMed:23403053, ECO:0000269\|PubMed:24130766}.
Function	G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose- stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (PubMed:23335512). Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway. {ECO:0000269\|PubMed:12629551, ECO:0000269\|PubMed:16044321, ECO:0000269\|PubMed:17395749, ECO:0000269\|PubMed:18559658, ECO:0000269\|PubMed:23335512, ECO:0000269\|PubMed:23403053, ECO:0000269\|PubMed:24130766}.
Similarity	Belongs to the G-protein coupled receptor 1 family. {ECO:0000255\|PROSITE-ProRule:PRU00521}.
Subcellular Location	Cell membrane {ECO:0000269\|PubMed:16044321, ECO:0000269\|PubMed:24130766}; Multi-pass membrane protein {ECO:0000255}.
Tissue Specificity	Expressed in pancreatic islet beta cells (at protein level) (PubMed:16044321). Expressed in pancreatic islet beta cells. {ECO:0000269\|PubMed:12496284, ECO:0000269\|PubMed:16044321}.

Unique RefSeq proteins for LMP003243 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
110835701	RefSeq	NP_918946	300	free fatty acid receptor 1

Identical Sequences to LMP003243 proteins

Reference	Database	Accession	Length	Protein Name
GI:110835701	DBBJ	BAC82555.1	300	GPR40 [Mus musculus]
GI:110835701	GenBank	AAI32015.1	300	Free fatty acid receptor 1 [Mus musculus]
GI:110835701	GenBank	ABU33414.1	300	Sequence 4 from patent US 7241579
GI:110835701	GenBank	AAI38100.1	300	Free fatty acid receptor 1 [Mus musculus]
GI:110835701	GenBank	AEI20900.1	300	Sequence 1 from patent US 7960369
GI:110835701	SwissProt	Q76JU9.1	300	RecName: Full=Free fatty acid receptor 1; AltName: Full=G-protein coupled receptor 40 [Mus musculus]

Related Sequences to LMP003243 proteins

Reference	Database	Accession	Length	Protein Name
GI:110835701	DBBJ	BAC82554.1	300	GPR40 [Rattus norvegicus]
GI:110835701	GenBank	AAN03478.1	300	GPR40 [Mus musculus]
GI:110835701	GenBank	AEI20901.1	300	Sequence 3 from patent US 7960369
GI:110835701	GenBank	AFQ84287.1	300	Sequence 4 from patent US 8252521
GI:110835701	RefSeq	NP_695216.1	300	free fatty acid receptor 1 [Rattus norvegicus]
GI:110835701	SwissProt	Q8K3T4.1	300	RecName: Full=Free fatty acid receptor 1; AltName: Full=G-protein coupled receptor 40 [Rattus norvegicus]