Gene/Proteome Database (LMPD)
LMPD ID
LMP002742
Gene ID
Species
Mus musculus (Mouse)
Gene Name
diacylglycerol kinase, theta
Gene Symbol
Synonyms
110kDa; DAGK; DAGK7; Dagk4; Dgkd
Alternate Names
diacylglycerol kinase theta; DGK-theta; Dgk theta; DAG kinase theta; diglyceride kinase theta; diacylglycerol kinase, delta (110 kDa)
Chromosome
5
Map Location
5 F|5 53.24 cM
EC Number
2.7.1.107
Proteins
| diacylglycerol kinase theta | |
|---|---|
| Refseq ID | NP_950176 |
| Protein GI | 39841023 |
| UniProt ID | Q6P5E8 |
| mRNA ID | NM_199011 |
| Length | 934 |
| RefSeq Status | PROVISIONAL |
| MAAAAEPGARTWPGSGSPRLGSPAGSPVLGISGRTRPGSGPERTSRAIGSAAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKQVKTPCTSIAPSLVRVPVAHCFGSLGLYKRKFCVVCRKSLEVPAFRCEVCELHVHPDCVPFACSDCRQCHQDGQQDYDTYHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSVCSTALAPECTFGRLRSMVLPPSCVRLLSRNFSKMHCFRIPETMVLELGDGDDGVDGSAAIGTGREVLTATESTKQTLKIFDGNDSMRKNQFRLVTVSRLARNEEVMEAALRAYYISEDPKDFQLQALPLSGNAQALGKAGTTEEEASKGSCPRDSVPEAWVIRSLPRTQEILKIYPGWLKVGVAYVSIRVNSQSTARSVVQEVLPLFGQQVEDKERFQLIEVLMSSRQVQRTVLADEEPLLDRLWDIRQTSVRQVSQTRFYVAETRATAPRVSLFVGGLPPGLSPQDYSNLLHEAMATKAAVVSVSHVYSLQGAVILDVTCFAEAERLYMLARDTAVHGRPLTALVLPDVLHTKLPPDCCPLLVFVNPKSGGLKGRELLCSFRKLLNPHQVFELTNGGPLPGFHLFSQVPSFRVLVCGGDGTVGWVLAALEETRRHLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLVSVDEADAVLMDRWTILLDAHEIDSTENNVVETEPPKIVQMNNYCGIGIDAELSLDFHQAREEEPGKFTSRFHNKGVYVRVGLQKISHSRSLHKEIRLQVEQQEVELPSIEGLIFINIPSWGSGADLWGSDNDSRFEKPRIDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISATAPKVHMLRKAKQKPRKAGANRDTRVDTLPAPEGNPL | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005856 | IEA:UniProtKB-KW | C | cytoskeleton |
| GO:0005829 | IEA:Ensembl | C | cytosol |
| GO:0016607 | IEA:Ensembl | C | nuclear speck |
| GO:0005886 | IEA:UniProtKB-KW | C | plasma membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0003951 | IEA:InterPro | F | NAD+ kinase activity |
| GO:0004143 | IEA:UniProtKB-EC | F | diacylglycerol kinase activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0019933 | IEA:Ensembl | P | cAMP-mediated signaling |
| GO:0070528 | IEA:Ensembl | P | protein kinase C signaling |
| GO:0007205 | IEA:InterPro | P | protein kinase C-activating G-protein coupled receptor signaling pathway |
| GO:0006357 | IEA:Ensembl | P | regulation of transcription from RNA polymerase II promoter |
| GO:0033198 | IEA:Ensembl | P | response to ATP |
| GO:0070493 | IEA:Ensembl | P | thrombin receptor signaling pathway |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR016064 | ATP-NAD kinase-like domain |
| IPR000756 | Diacylglycerol kinase, accessory domain |
| IPR001206 | Diacylglycerol kinase, catalytic domain |
| IPR020454 | Diacylglycerol/phorbol-ester binding |
| IPR002219 | Protein kinase C-like, phorbol ester/diacylglycerol-binding domain |
| IPR000159 | Ras-association |
| IPR029071 | Ubiquitin-related domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q6P5E8-1; Sequence=Displayed; Name=2; IsoId=Q6P5E8-2; Sequence=VSP_037832; Note=No experimental confirmation available.; |
| Catalytic Activity | ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2- diacyl-sn-glycerol 3-phosphate. |
| Enzyme Regulation | Inactivated by binding to RHOA. Not inhibited by phosphatidylserine (By similarity). {ECO:0000250}. |
| Function | Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity (By similarity). {ECO:0000250}. |
| Ptm | Phosphorylated by PRKCE and PRKCH in vitro. {ECO:0000250}. |
| Similarity | Belongs to the eukaryotic diacylglycerol kinase family. {ECO:0000305}. |
| Similarity | Contains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}. |
| Similarity | Contains 1 Ras-associating domain. {ECO:0000255|PROSITE-ProRule:PRU00166}. |
| Similarity | Contains 3 phorbol-ester/DAG-type zinc fingers. {ECO:0000255|PROSITE-ProRule:PRU00226}. |
| Subcellular Location | Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Nucleus {ECO:0000250}. Nucleus speckle {ECO:0000250}. Note=Translocates to the nucleus in response to thrombin stimulation. Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH. {ECO:0000250}. |
| Subunit | Interacts with RHOA (constitutively activated, GTP- bound); the interaction inhibits DGKQ. Interacts with PRKCE. Interacts with PRKCH. Interacts with PLCB1. Interacts with NR5A1 (By similarity). {ECO:0000250}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP002742 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 39841023 | RefSeq | NP_950176 | 934 | diacylglycerol kinase theta |
Identical Sequences to LMP002742 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:39841023 | GenBank | AAH62929.1 | 934 | Diacylglycerol kinase, theta [Mus musculus] |
| GI:39841023 | SwissProt | Q6P5E8.1 | 934 | RecName: Full=Diacylglycerol kinase theta; Short=DAG kinase theta; AltName: Full=Diglyceride kinase theta; Short=DGK-theta [Mus musculus] |
Related Sequences to LMP002742 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:39841023 | GenBank | EDL20093.1 | 934 | diacylglycerol kinase, theta, isoform CRA_a [Mus musculus] |
| GI:39841023 | GenBank | EDL84024.1 | 937 | rCG57232, isoform CRA_b [Rattus norvegicus] |
| GI:39841023 | RefSeq | NP_001185733.1 | 937 | diacylglycerol kinase theta [Rattus norvegicus] |
| GI:39841023 | RefSeq | XP_003502424.1 | 947 | PREDICTED: diacylglycerol kinase theta isoform X1 [Cricetulus griseus] |
| GI:39841023 | RefSeq | XP_005359728.1 | 936 | PREDICTED: diacylglycerol kinase theta [Microtus ochrogaster] |
| GI:39841023 | RefSeq | XP_006985866.1 | 936 | PREDICTED: diacylglycerol kinase theta [Peromyscus maniculatus bairdii] |