Gene/Proteome Database (LMPD)
LMPD ID
LMP002740
Gene ID
Species
Homo sapiens (Human)
Gene Name
ceramide kinase
Gene Symbol
Synonyms
LK4; dA59H18.2; dA59H18.3; hCERK
Alternate Names
ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase
Chromosome
22
Map Location
22q13.31
EC Number
2.7.1.138
Summary
CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation (Kim et al., 2006 [PubMed 16488390]).[supplied by OMIM, Mar 2008]
Orthologs
Proteins
| ceramide kinase | |
|---|---|
| Refseq ID | NP_073603 |
| Protein GI | 20336726 |
| UniProt ID | Q8TCT0 |
| mRNA ID | NM_022766 |
| Length | 537 |
| RefSeq Status | VALIDATED |
| MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGAPGADACSVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWAQVTFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIYERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMFSEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0016021 | IDA:UniProtKB | C | integral component of membrane |
| GO:0005739 | IEA:Ensembl | C | mitochondrion |
| GO:0005886 | TAS:Reactome | C | plasma membrane |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0003951 | IEA:InterPro | F | NAD+ kinase activity |
| GO:0001729 | IDA:UniProtKB | F | ceramide kinase activity |
| GO:0004143 | IEA:InterPro | F | diacylglycerol kinase activity |
| GO:0000287 | IDA:UniProtKB | F | magnesium ion binding |
| GO:0006672 | IDA:UniProtKB | P | ceramide metabolic process |
| GO:0006687 | TAS:Reactome | P | glycosphingolipid metabolic process |
| GO:0046834 | IDA:GOC | P | lipid phosphorylation |
| GO:0007205 | IEA:InterPro | P | protein kinase C-activating G-protein coupled receptor signaling pathway |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0006665 | TAS:Reactome | P | sphingolipid metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa00600 | Sphingolipid metabolism |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_116105 | Glycosphingolipid metabolism |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8TCT0-1; Sequence=Displayed; Name=2; IsoId=Q8TCT0-2; Sequence=VSP_056944; Note=No experimental confirmation available; |
| Biophysicochemical Properties | pH dependence: Optimum pH is 6.0-7.5.; |
| Catalytic Activity | ATP + ceramide = ADP + ceramide 1-phosphate. |
| Cofactor | Name=Ca(2+); Xref=ChEBI |
| Cofactor | Name=Mg(2+); Xref=ChEBI |
| Enzyme Regulation | Inhibited by sulfatide. |
| Function | Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides. |
| Similarity | Contains 1 DAGKc domain. {ECO |
| Subcellular Location | Cytoplasm . Membrane ; Peripheral membrane protein . |
| Tissue Specificity | High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung. |
Identical and Related Proteins
Unique RefSeq proteins for LMP002740 (as displayed in Record Overview)
Identical Sequences to LMP002740 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:20336726 | DBBJ | BAF84165.1 | 537 | unnamed protein product [Homo sapiens] |
| GI:20336726 | DBBJ | BAG72490.1 | 537 | ceramide kinase, partial [synthetic construct] |
| GI:20336726 | EMBL | CAK54679.1 | 537 | CERK, partial [synthetic construct] |
| GI:20336726 | GenBank | AAI26941.1 | 537 | Ceramide kinase [Homo sapiens] |
| GI:20336726 | GenBank | EAW73439.1 | 537 | ceramide kinase, isoform CRA_b [Homo sapiens] |
| GI:20336726 | GenBank | EAW73441.1 | 537 | ceramide kinase, isoform CRA_b [Homo sapiens] |
Related Sequences to LMP002740 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:20336726 | GenBank | JAA13843.1 | 537 | ceramide kinase [Pan troglodytes] |
| GI:20336726 | GenBank | JAA13844.1 | 537 | ceramide kinase [Pan troglodytes] |
| GI:20336726 | GenBank | JAA13845.1 | 537 | ceramide kinase [Pan troglodytes] |
| GI:20336726 | GenBank | JAA13846.1 | 537 | ceramide kinase [Pan troglodytes] |
| GI:20336726 | GenBank | JAA13847.1 | 537 | ceramide kinase [Pan troglodytes] |
| GI:20336726 | GenBank | JAA13848.1 | 537 | ceramide kinase [Pan troglodytes] |