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Comment Type
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Description
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Alternative Products
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Event=Alternative splicing; Named isoforms=2; Name=1 {ECO:0000269|PubMed:12117804, ECO:0000269|PubMed:14701816, ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:16141072}; IsoId=Q8K4D7-1; Sequence=Displayed; Name=2 ; IsoId=Q8K4D7-2; Sequence=VSP_052863;
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Catalytic Activity
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1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
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Cofactor
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Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence= ;
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Domain
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The EF-hand and C2 domains are essential for triggering Ca(2+) oscillating activity and the regulation of PLCZ1 enzyme activity. {ECO:0000269|PubMed:15790568, ECO:0000269|PubMed:16000311, ECO:0000269|PubMed:16854985, ECO:0000269|PubMed:18028898}.
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Domain
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The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization. {ECO:0000269|PubMed:15790568, ECO:0000269|PubMed:16000311, ECO:0000269|PubMed:16854985, ECO:0000269|PubMed:18028898}.
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Function
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The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. {ECO:0000250|UniProtKB:Q86YW0, ECO:0000269|PubMed:12117804, ECO:0000269|PubMed:14701816, ECO:0000269|PubMed:15159452, ECO:0000269|PubMed:15385165, ECO:0000269|PubMed:15790568, ECO:0000269|PubMed:16854985, ECO:0000269|PubMed:17933795, ECO:0000269|PubMed:18028898, ECO:0000269|PubMed:18322275, ECO:0000305}.
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Miscellaneous
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Transgenic mice, where broad ectopic expression is forced, initially appear healthy and their oocytes undergo unperturbed meiotic maturation to metaphase II but subsequently exhibit autonomous intracellular free calcium oscillations, second polar body extrusion, pronucleus formation and parthenogenetic development. Transgenic males remained largely asymptomatic, whereas transgenic females develop abdominal swellings caused by benign ovarian teratomas.
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Similarity
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Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}.
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Similarity
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Contains 1 EF-hand domain. {ECO:0000255|PROSITE- ProRule:PRU00448}.
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Similarity
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Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}.
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Similarity
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Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}.
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Subcellular Location
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Nucleus {ECO:0000269|PubMed:14701816, ECO:0000269|PubMed:15159452, ECO:0000269|PubMed:15385165, ECO:0000269|PubMed:15809052, ECO:0000269|PubMed:18322275}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:14701816, ECO:0000269|PubMed:15159452, ECO:0000269|PubMed:15385165, ECO:0000269|PubMed:15809052, ECO:0000269|PubMed:18322275}. Note=Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronuclei at interphase following meiosis and mitosis. {ECO:0000269|PubMed:14701816, ECO:0000269|PubMed:15159452, ECO:0000269|PubMed:15385165, ECO:0000269|PubMed:15809052, ECO:0000269|PubMed:18322275}.
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Subunit
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Interacts via its C2 domain with PtdIns(3)P and, to a lesser extent, PtdIns(5)P in vitro.
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Tissue Specificity
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Highly expressed in postpuberal testis, where expression is sperm cell-specific. Also expressed in brain of both sexes.
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