Gene/Proteome Database (LMPD)

LMPD ID
LMP002433
Gene ID
242
Species
Homo sapiens (Human)
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
ALOX12B
Synonyms
12R-LOX; ARCI2
Alternate Names
arachidonate 12-lipoxygenase, 12R-type; 12R-lipoxygenase; epidermis-type lipoxygenase 12
Chromosome
17
Map Location
17p13.1
EC Number
1.13.11.-
Summary
This gene encodes an enzyme involved in the converstion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for ALOX12B

Proteins

arachidonate 12-lipoxygenase, 12R-type
Refseq ID NP_001130
Protein GI 4502053
UniProt ID O75342
mRNA ID NM_001139
Length 701
RefSeq Status REVIEWED
MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISI

Gene Information

Entrez Gene ID
242
Gene Name
arachidonate 12-lipoxygenase, 12R type
Gene Symbol
ALOX12B
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 TAS:Reactome C cytosol
GO:0004052 IMP:UniProtKB F arachidonate 12-lipoxygenase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:1990136 ISS:UniProtKB F linoleate 9S-lipoxygenase activity
GO:0016702 IDA:UniProtKB F oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0019369 IMP:UniProtKB P arachidonic acid metabolic process
GO:0046513 ISS:UniProtKB P ceramide biosynthetic process
GO:0061436 ISS:UniProtKB P establishment of skin barrier
GO:0051122 ISS:UniProtKB P hepoxilin biosynthetic process
GO:0043651 ISS:UniProtKB P linoleic acid metabolic process
GO:0019372 IDA:UniProtKB P lipoxygenase pathway
GO:0055114 IDA:UniProtKB P oxidation-reduction process
GO:0043410 IDA:UniProtKB P positive regulation of MAPK cascade
GO:0010628 IMP:UniProtKB P positive regulation of gene expression
GO:0070257 IMP:UniProtKB P positive regulation of mucus secretion
GO:0006497 ISS:UniProtKB P protein lipidation
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0006665 IDA:UniProtKB P sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa00590 Arachidonic acid metabolism
hsa04726 Serotonergic synapse

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_147851 Arachidonic acid metabolism
REACT_150201 Synthesis of 12-eicosatetraenoic acid derivatives

Domain Information

InterPro Annotations

Accession Description
IPR008976 Lipase/lipooxygenase, PLAT/LH2
IPR000907 Lipoxygenase
IPR013819 Lipoxygenase, C-terminal
IPR020834 Lipoxygenase, conserved site
IPR020833 Lipoxygenase, iron binding site
IPR001885 Lipoxygenase, mammalian
IPR001024 PLAT/LH2 domain

UniProt Annotations

Entry Information

Gene Name
arachidonate 12-lipoxygenase, 12R type
Protein Entry
LX12B_HUMAN
UniProt ID
O75342
Species
Human

Comments

Comment Type Description
Catalytic Activity Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate.
Cofactor Name=Fe cation; Xref=ChEBI
Disease Ichthyosis, congenital, autosomal recessive 2 (ARCI2) [MIM
Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO
Interaction Q9BYJ1:ALOXE3; NbExp=2; IntAct=EBI-6925925, EBI-6925949;
Pathway Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
Pathway Lipid metabolism; sphingolipid metabolism.
Similarity Belongs to the lipoxygenase family.
Similarity Contains 1 PLAT domain. {ECO
Similarity Contains 1 lipoxygenase domain. {ECO
Subcellular Location Cytoplasm {ECO
Tissue Specificity Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue.
Web Resource Name=Protein Spotlight; Note=about water - Issue 153 of September 2013; URL="http://web.expasy.org/spotlight/back_issues/153/";

Identical and Related Proteins

Unique RefSeq proteins for LMP002433 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
4502053 RefSeq NP_001130 701 arachidonate 12-lipoxygenase, 12R-type

Identical Sequences to LMP002433 proteins

Reference Database Accession Length Protein Name
GI:4502053 DBBJ BAJ21000.1 701 arachidonate 12-lipoxygenase, 12R type, partial [synthetic construct]
GI:4502053 GenBank AAQ32814.1 701 Sequence 2 from patent US 6569644
GI:4502053 GenBank EAW90095.1 701 arachidonate 12-lipoxygenase, 12R type [Homo sapiens]
GI:4502053 GenBank ACM80528.1 701 Sequence 6026 from patent US 6812339
GI:4502053 GenBank AHE01970.1 701 Sequence 59819 from patent US 8586006
GI:4502053 GenBank AIC48254.1 701 ALOX12B, partial [synthetic construct]

Related Sequences to LMP002433 proteins

Reference Database Accession Length Protein Name
GI:4502053 GenBank EHH24497.1 701 Arachidonate 12-lipoxygenase, 12R-type [Macaca mulatta]
GI:4502053 GenBank EHH57699.1 701 Arachidonate 12-lipoxygenase, 12R-type [Macaca fascicularis]
GI:4502053 RefSeq XP_511864.2 701 PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Pan troglodytes]
GI:4502053 RefSeq XP_003818099.1 701 PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Pan paniscus]
GI:4502053 RefSeq XP_003912343.1 701 PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Papio anubis]
GI:4502053 RefSeq XP_004058584.1 701 PREDICTED: arachidonate 12-lipoxygenase, 12R-type [Gorilla gorilla gorilla]