Gene/Proteome Database (LMPD)
LMPD ID
LMP001791
Gene ID
Species
Homo sapiens (Human)
Gene Name
phospholipase C, zeta 1
Gene Symbol
Synonyms
NYD-SP27; PLC-zeta-1; PLCzeta
Chromosome
12
Map Location
12p12.3
EC Number
3.1.4.11
Summary
The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Orthologs
Proteins
| 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 | |
|---|---|
| Refseq ID | NP_149114 |
| Protein GI | 25188201 |
| UniProt ID | Q86YW0 |
| mRNA ID | NM_033123 |
| Length | 608 |
| RefSeq Status | REVIEWED |
| MEMRWFLSKIQDDFRGGKINLEKTQRLLEKLDIRCSYIHVKQIFKDNDRLKQGRITIEEFRAIYRIITHREEIIEIFNTYSENRKILLASNLAQFLTQEQYAAEMSKAIAFEIIQKYEPIEEVRKAHQMSLEGFTRYMDSRECLLFKNECRKVYQDMTHPLNDYFISSSHNTYLVSDQLLGPSDLWGYVSALVKGCRCLEIDCWDGAQNEPVVYHGYTLTSKLLFKTVIQAIHKYAFMTSDYPVVLSLENHCSTAQQEVMADNLQATFGESLLSDMLDDFPDTLPSPEALKFKILVKNKKIGTLKETHERKGSDKRGDNQDKETGVKKLPGVMLFKKKKTRKLKIALALSDLVIYTKAEKFKSFQHSRLYQQFNENNSIGETQARKLSKLRVHEFIFHTRKFITRIYPKATRADSSNFNPQEFWNIGCQMVALNFQTPGLPMDLQNGKFLDNGGSGYILKPHFLRESKSYFNPSNIKEGMPITLTIRLISGIQLPLTHSSSNKGDSLVIIEVFGVPNDQMKQQTRVIKKNAFSPRWNETFTFIIHVPELALIRFVVEGQGLIAGNEFLGQYTLPLLCMNKGYRRIPLFSRMGESLEPASLFVYVWYVR | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0005634 | IEA:UniProtKB-KW | C | nucleus |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0004435 | IEA:UniProtKB-EC | F | phosphatidylinositol phospholipase C activity |
| GO:0004871 | IEA:UniProtKB-KW | F | signal transducer activity |
| GO:0006816 | IEA:Ensembl | P | calcium ion transport |
| GO:0007343 | IEA:Ensembl | P | egg activation |
| GO:0043647 | TAS:Reactome | P | inositol phosphate metabolic process |
| GO:0035556 | IEA:InterPro | P | intracellular signal transduction |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0007275 | IEA:UniProtKB-KW | P | multicellular organismal development |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa04020 | Calcium signaling pathway |
| hsa04114 | Oocyte meiosis |
| hsa04919 | Thyroid hormone signaling pathway |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_150312 | Synthesis of IP3 and IP4 in the cytosol |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR028395 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 |
| IPR000008 | C2 domain |
| IPR002048 | EF-hand domain |
| IPR011992 | EF-hand domain pair |
| IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain |
| IPR001192 | Phosphoinositide phospholipase C family |
| IPR000909 | Phospholipase C, phosphatidylinositol-specific , X domain |
| IPR001711 | Phospholipase C, phosphatidylinositol-specific, Y domain |
| IPR015359 | Phospholipase C, phosphoinositol-specific, EF-hand-like |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=3; Name=1 {ECO |
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Name=Ca(2+); Xref=ChEBI |
| Domain | The EF-hand and C2 domains are essential for triggering Ca(2+) oscillating activity and the regulation of PLCZ1 enzyme activity. |
| Domain | The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization. |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)- dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg- activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. {ECO |
| Similarity | Contains 1 C2 domain. {ECO |
| Similarity | Contains 1 EF-hand domain. {ECO |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO |
| Subcellular Location | Nucleus . Cytoplasm, perinuclear region . Note=Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis (By similarity). |
| Subunit | Interacts via its C2 domain with PtdIns(3)P and, to a lesser extent, PtdIns(5)P in vitro. |
| Tissue Specificity | Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic- duct cells from patients with cystic fibrosis. |
Identical and Related Proteins
Unique RefSeq proteins for LMP001791 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 25188201 | RefSeq | NP_149114 | 608 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 |
Identical Sequences to LMP001791 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:25188201 | EMBL | CBU87809.1 | 608 | unnamed protein product [Homo sapiens] |
| GI:25188201 | EMBL | CBU96756.1 | 608 | unnamed protein product [Homo sapiens] |
| GI:25188201 | GenBank | AED62142.1 | 608 | Sequence 24 from patent US 7902154 |
| GI:25188201 | GenBank | AGX54744.1 | 608 | Sequence 9844 from patent US 8541208 |
| GI:25188201 | GenBank | AGX64184.1 | 608 | Sequence 28478 from patent US 8541208 |
| GI:25188201 | GenBank | AHD79551.1 | 608 | Sequence 29195 from patent US 8586006 |
Related Sequences to LMP001791 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:25188201 | EMBL | CAD35472.1 | 608 | unnamed protein product [Homo sapiens] |
| GI:25188201 | GenBank | ACH36769.1 | 608 | Sequence 43 from patent US 7411054 |
| GI:25188201 | GenBank | ADM05352.1 | 608 | Sequence 43 from patent US 7732182 |
| GI:25188201 | RefSeq | XP_004052884.1 | 608 | PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 [Gorilla gorilla gorilla] |
| GI:25188201 | RefSeq | XP_005253576.1 | 649 | PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X1 [Homo sapiens] |
| GI:25188201 | RefSeq | XP_005253577.1 | 649 | PREDICTED: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 isoform X2 [Homo sapiens] |