Gene/Proteome Database (LMPD)

LMPD ID
LMP001503
Gene ID
13382
Species
Mus musculus (Mouse)
Gene Name
dihydrolipoamide dehydrogenase
Gene Symbol
Dld
Synonyms
-
Alternate Names
dihydrolipoyl dehydrogenase, mitochondrial; branched chain alpha-keto acid dehydrogenase complex subunit E3
Chromosome
12
Map Location
12 A3|12 13.43 cM
EC Number
1.8.1.4
Summary
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. [provided by RefSeq, Jan 2014]
Orthologs
View orthologs and multiple alignments for Dld

Proteins

dihydrolipoyl dehydrogenase, mitochondrial precursor
Refseq ID NP_031887
Protein GI 31982856
UniProt ID O08749
mRNA ID NM_007861
Length 509
RefSeq Status REVIEWED
MQSWSRVYRSLAKKGHFNRISHGLQGVSSVPLRTYADQPIEADVTVIGSGPGGYVAAIKSAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEIPEVRLNLEKMMEQKHSAVKALTGGIAHLFKQNKVVHVNGFGKITGKNQVTATKADGSTQVIDTKNILVATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQRQGFKFKLNTKVTGATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNNRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEFKIGKFPFAANSRAKTNADTDGMVKILGHKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAAAFGKPINF
transit_peptide: 1..35 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (By similarity); propagated from UniProtKB/Swiss-Prot (O08749.2) calculated_mol_wt: 4048 peptide sequence: MQSWSRVYRSLAKKGHFNRISHGLQGVSSVPLRTY mat_peptide: 36..509 product: Dihydrolipoyl dehydrogenase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (O08749.2) calculated_mol_wt: 50243 peptide sequence: ADQPIEADVTVIGSGPGGYVAAIKSAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEIPEVRLNLEKMMEQKHSAVKALTGGIAHLFKQNKVVHVNGFGKITGKNQVTATKADGSTQVIDTKNILVATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQRQGFKFKLNTKVTGATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNNRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEFKIGKFPFAANSRAKTNADTDGMVKILGHKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAAAFGKPINF

Gene Information

Entrez Gene ID
13382
Gene Name
dihydrolipoamide dehydrogenase
Gene Symbol
Dld
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0043159 IDA:MGI C acrosomal matrix
GO:0005929 IDA:MGI C cilium
GO:0005739 IDA:MGI C mitochondrion
GO:0005634 IEA:Ensembl C nucleus
GO:0004148 IMP:MGI F dihydrolipoyl dehydrogenase activity
GO:0050660 IEA:InterPro F flavin adenine dinucleotide binding
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0007369 IMP:MGI P gastrulation
GO:0006120 IMP:MGI P mitochondrial electron transport, NADH to ubiquinone
GO:0006508 IDA:MGI P proteolysis
GO:0042391 IMP:MGI P regulation of membrane potential
GO:0048240 IDA:MGI P sperm capacitation

KEGG Pathway Links

KEGG Pathway ID Description
mmu00020 Citrate cycle (TCA cycle)
mmu00260 Glycine, serine and threonine metabolism
mmu00010 Glycolysis / Gluconeogenesis

Domain Information

InterPro Annotations

Accession Description
IPR006258 Dihydrolipoamide dehydrogenase
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase
IPR016156 FAD/NAD-linked reductase, dimerisation domain
IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain
IPR012999 Pyridine nucleotide-disulphide oxidoreductase, class I, active site
IPR004099 Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain

UniProt Annotations

Entry Information

Gene Name
dihydrolipoamide dehydrogenase
Protein Entry
DLDH_MOUSE
UniProt ID
O08749
Species
Mouse

Comments

Comment Type Description
Catalytic Activity Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH.
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250}; Note=Binds 1 FAD per subunit. {ECO:0000250};
Function Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Miscellaneous The active site is a redox-active disulfide bond.
Ptm Acetylation of Lys-127 is observed in liver mitochondria from fasted mice but not from fed mice. {ECO:0000269|PubMed:23576753, ECO:0000269|PubMed:23806337}.
Ptm Tyrosine phosphorylated. {ECO:0000250}.
Similarity Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. {ECO:0000305}.
Subcellular Location Mitochondrion matrix.
Subunit Homodimer. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by non-covalent bonds (By similarity). {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001503 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
31982856 RefSeq NP_031887 509 dihydrolipoyl dehydrogenase, mitochondrial precursor

Identical Sequences to LMP001503 proteins

Reference Database Accession Length Protein Name
GI:31982856 DBBJ BAE43405.1 509 unnamed protein product [Mus musculus]
GI:31982856 GenBank AAH03368.1 509 Dihydrolipoamide dehydrogenase [Mus musculus]
GI:31982856 SwissProt O08749.2 509 RecName: Full=Dihydrolipoyl dehydrogenase, mitochondrial; AltName: Full=Dihydrolipoamide dehydrogenase; Flags: Precursor [Mus musculus]

Related Sequences to LMP001503 proteins

Reference Database Accession Length Protein Name
GI:31982856 DBBJ BAE31961.1 509 unnamed protein product [Mus musculus]
GI:31982856 DBBJ BAE22867.1 509 unnamed protein product [Mus musculus]
GI:31982856 DBBJ BAE40693.1 509 unnamed protein product [Mus musculus]
GI:31982856 GenBank AAC53170.1 509 dihydrolipoamide dehydrogenase [Mus musculus]
GI:31982856 GenBank EDL36917.1 547 dihydrolipoamide dehydrogenase, partial [Mus musculus]
GI:31982856 SwissProt Q6P6R2.1 509 RecName: Full=Dihydrolipoyl dehydrogenase, mitochondrial; AltName: Full=Dihydrolipoamide dehydrogenase; Flags: Precursor [Rattus norvegicus]