Gene/Proteome Database (LMPD)

LMPD ID
LMP001130
Gene ID
11364
Species
Mus musculus (Mouse)
Gene Name
acyl-Coenzyme A dehydrogenase, medium chain
Gene Symbol
Acadm
Synonyms
AU018656; MCAD
Alternate Names
medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Chromosome
3
Map Location
3 H3|3 78.77 cM
EC Number
1.3.8.7
Summary
This gene encodes a homotetrameric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C6- and C12-acylCoA. In mice, deficiency of this gene can cause neonatal mortality as well as fasting and cold intolerance. This gene has multiple, intronless pseudogenes. [provided by RefSeq, Nov 2012]
Orthologs
View orthologs and multiple alignments for Acadm

Proteins

medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor
Refseq ID NP_031408
Protein GI 6680618
UniProt ID P45952
mRNA ID NM_007382
Length 421
RefSeq Status REVIEWED
MAAAFRRGCRVLRSVSHFECRTQHSKAAHKQEPGLGFSFELTEQQKEFQATARKFAREEIIPVAPEYDKSGEYPFPLIKRAWELGLINAHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVILAGNDQQKKKYLGRMTEQPMMCAYCVTEPSAGSDVAAIKTKAEKKGDEYVINGQKMWITNGGKANWYFLLARSNPDPKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGIAFEDVRVPKENVLIGEGAGFKIAMGAFDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYKN
transit_peptide: 1..25 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (By similarity); propagated from UniProtKB/Swiss-Prot (P45952.1) calculated_mol_wt: 2906 peptide sequence: MAAAFRRGCRVLRSVSHFECRTQHS mat_peptide: 26..421 product: Medium-chain specific acyl-CoA dehydrogenase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P45952.1) calculated_mol_wt: 43593 peptide sequence: KAAHKQEPGLGFSFELTEQQKEFQATARKFAREEIIPVAPEYDKSGEYPFPLIKRAWELGLINAHIPESCGGLGLGTFDACLITEELAYGCTGVQTAIEANSLGQMPVILAGNDQQKKKYLGRMTEQPMMCAYCVTEPSAGSDVAAIKTKAEKKGDEYVINGQKMWITNGGKANWYFLLARSNPDPKVPASKAFTGFIVEADTPGIHIGKKELNMGQRCSDTRGIAFEDVRVPKENVLIGEGAGFKIAMGAFDRTRPTVAAGAVGLAQRALDEATKYALDRKTFGKLLVEHQGVSFLLAEMAMKVELARLSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQIFGGYGFNTEYPVEKLMRDAKIYQIYEGTAQIQRLIIAREHIEKYKN

Gene Information

Entrez Gene ID
11364
Gene Name
acyl-Coenzyme A dehydrogenase, medium chain
Gene Symbol
Acadm
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0030424 IEA:Ensembl C axon
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0005739 IDA:MGI C mitochondrion
GO:0005634 IEA:Ensembl C nucleus
GO:0003995 ISS:UniProtKB F acyl-CoA dehydrogenase activity
GO:0050660 IEA:InterPro F flavin adenine dinucleotide binding
GO:0070991 IMP:BHF-UCL F medium-chain-acyl-CoA dehydrogenase activity
GO:0055007 IMP:MGI P cardiac muscle cell differentiation
GO:0045329 IEA:Ensembl P carnitine biosynthetic process
GO:0009437 IMP:MGI P carnitine metabolic process
GO:0019254 IMP:BHF-UCL P carnitine metabolic process, CoA-linked
GO:0006635 ISS:UniProtKB P fatty acid beta-oxidation
GO:0033539 IMP:BHF-UCL P fatty acid beta-oxidation using acyl-CoA dehydrogenase
GO:0005978 IMP:BHF-UCL P glycogen biosynthetic process
GO:0007507 IMP:MGI P heart development
GO:0001889 IMP:MGI P liver development
GO:0051793 IEA:Ensembl P medium-chain fatty acid catabolic process
GO:0051791 IMP:BHF-UCL P medium-chain fatty acid metabolic process
GO:0006082 IMP:MGI P organic acid metabolic process
GO:0009791 IMP:MGI P post-embryonic development
GO:0006111 IMP:BHF-UCL P regulation of gluconeogenesis
GO:0009409 IMP:MGI P response to cold
GO:0042594 IMP:MGI P response to starvation

KEGG Pathway Links

KEGG Pathway ID Description
mmu01200 Carbon metabolism
mmu03320 PPAR signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR006089 Acyl-CoA dehydrogenase, conserved site
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal
IPR013786 Acyl-CoA dehydrogenase/oxidase, N-terminal
IPR009100 Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain

UniProt Annotations

Entry Information

Gene Name
acyl-Coenzyme A dehydrogenase, medium chain
Protein Entry
ACADM_MOUSE
UniProt ID
P45952
Species
Mouse

Comments

Comment Type Description
Catalytic Activity A medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein.
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
Function This enzyme is specific for acyl chain lengths of 4 to 16.
Miscellaneous A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.
Pathway Lipid metabolism; mitochondrial fatty acid beta- oxidation.
Ptm Acetylation at Lys-307 and Lys-311 in proximity of the cofactor-binding sites reduces catalytic activity. These sites are deacetylated by SIRT3 (By similarity). {ECO:0000250}.
Similarity Belongs to the acyl-CoA dehydrogenase family. {ECO:0000305}.
Subcellular Location Mitochondrion matrix {ECO:0000250}.
Subunit Homotetramer. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP001130 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6680618 RefSeq NP_031408 421 medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor

Identical Sequences to LMP001130 proteins

Reference Database Accession Length Protein Name
GI:6680618 GenBank EDL11896.1 421 acyl-Coenzyme A dehydrogenase, medium chain [Mus musculus]
GI:6680618 GenBank AEQ84558.1 421 Sequence 51 from patent US 8039587
GI:6680618 GenBank AEU53421.1 421 Sequence 51 from patent US 8062891
GI:6680618 GenBank AGO72374.1 421 Sequence 51 from patent US 8470972
GI:6680618 GenBank AGU32365.1 421 Sequence 51 from patent US 8507277
GI:6680618 GenBank AGX48269.1 421 Sequence 51 from patent US 8541550

Related Sequences to LMP001130 proteins

Reference Database Accession Length Protein Name
GI:6680618 GenBank AAA40670.1 421 acyl-CoA dehydrogenase medium subunit (EC 1.3.99.3) [Rattus norvegicus]
GI:6680618 GenBank AAH13498.1 421 Acyl-Coenzyme A dehydrogenase, medium chain [Mus musculus]
GI:6680618 GenBank EDL82532.1 421 acetyl-Coenzyme A dehydrogenase, medium chain [Rattus norvegicus]
GI:6680618 RefSeq NP_058682.2 421 medium-chain specific acyl-CoA dehydrogenase, mitochondrial precursor [Rattus norvegicus]
GI:6680618 RefSeq XP_007648396.1 421 PREDICTED: medium-chain specific acyl-CoA dehydrogenase, mitochondrial [Cricetulus griseus]
GI:6680618 SwissProt P08503.1 421 RecName: Full=Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=MCAD; Flags: Precursor [Rattus norvegicus]