Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo2-Lipid A

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technical replicates
LM_ID Name 0 hrs 6 hrs 24 hrs Units
  LMFA01030283 10Z-heptadecenoic acid 2.7 3.2 3.4 pmol/1E6 cells
  LMFA01030159 11,14,17-eicosatrienoic acid 5.1 6.1 6 pmol/1E6 cells
  LMFA01030130 11,14-eicosadienoic acid 6.5 7.3 7.6 pmol/1E6 cells
  LMFA01030408 13,16,19-docosatrienoic acid - 0.02 0.28 pmol/1E6 cells
  LMFA01030132 13,16-docosadienoic acid 1.4 1.6 1.8 pmol/1E6 cells
  LMFA01030759 5,8,11,14,17-eicosapentaenoic acid 5.6 6.2 6.9 pmol/1E6 cells
  LMFA01030157 5,8,11-eicosatrienoic acid 18 19 20 pmol/1E6 cells
  LMFA01030184 7,10,13,16,19-docosapentaenoic acid 72 77 81 pmol/1E6 cells
  LMFA01030178 7Z,10Z,13Z,16Z-docosatetraenoic acid 43 45 49 pmol/1E6 cells
  LMFA01030056 9Z-palmitoleic acid 69 76 81 pmol/1E6 cells
  LMFA01030152 alpha-linolenic acid 0.73 0.83 1.1 pmol/1E6 cells
  LMFA01010020 Arachidic acid 3.6 4.8 4.9 pmol/1E6 cells
  LMFA01030001 Arachidonic acid 180 200 210 pmol/1E6 cells
  LMFA01010022 Behenic acid 0.83 1.1 1.3 pmol/1E6 cells
  LMFA01030158 bishomo-gamma-linolenic acid 0.27 0.35 0.65 pmol/1E6 cells
  LMFA01010026 Cerotic acid - - 0.06 pmol/1E6 cells
  LMFA01030089 cis-erucic acid 0.87 1.1 1.2 pmol/1E6 cells
  LMFA01030092 cis-selacholeic acid 2.3 2.6 2.7 pmol/1E6 cells
  LMFA01030185 DHA 88 96 99 pmol/1E6 cells
  LMFA01030141 gamma-linolenic acid 0.83 0.85 1.2 pmol/1E6 cells
  LMFA01010012 Lauric acid 9.7 13 4.9 pmol/1E6 cells
  LMFA01010024 Lignoceric acid 1.4 1.7 1.7 pmol/1E6 cells
  LMFA01030120 Linoleic acid 88 94 98 pmol/1E6 cells
  LMFA01010017 Margaric acid 21 24 26 pmol/1E6 cells
  LMFA01010014 Myristic acid 180 190 200 pmol/1E6 cells
  LMFA01030002 Oleic acid 550 600 600 pmol/1E6 cells
  LMFA01010001 Palmitic acid 730 810 860 pmol/1E6 cells
  LMFA01010015 Pentadecanoic acid 29 33 33 pmol/1E6 cells
  LMFA01010018 Stearic acid 380 450 480 pmol/1E6 cells
  LMFA01030357 Stearidonic acid - - 0.12 pmol/1E6 cells
  LMFA01010023 Tricosanoic acid - - 0.14 pmol/1E6 cells

 

*:The units in the table and graphs correspond to the intensity of each analyte divided by the intensity of the corresponding deuterated internal standard. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles by extrapolating from the standard curve for that analyte and then normalized to the number of cells in a given sample as measured by the quantity of DNA in the well.