Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo2-Lipid A

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technical replicates
LM_ID Name 0 hrs 6 hrs 24 hrs Units
  LMST01020004 CE(14:0) 6 6.9 7.9 pmol/1E6 cells
  LMST01020021 CE(14:1) - - - pmol/1E6 cells
  LMST01020027 CE(15:0) 2.3 2.9 4.2 pmol/1E6 cells
  LMST01020022 CE(15:1) - - - pmol/1E6 cells
  LMST01020005 CE(16:0) 14 17 23 pmol/1E6 cells
  LMST01020006 CE(16:1) 35 53 47 pmol/1E6 cells
  LMST01020024 CE(16:2) 12 14 14 pmol/1E6 cells
  LMST01020026 CE(17:0) - - - pmol/1E6 cells
  LMST01020023 CE(17:1) 18 20 20 pmol/1E6 cells
  LMST01020007 CE(18:0) 7.2 14 12 pmol/1E6 cells
  LMST01020003 CE(18:1) 81 140 120 pmol/1E6 cells
  LMST01020008 CE(18:2) 31 41 34 pmol/1E6 cells
  LMST01020009 CE(18:3) 7.7 7.6 7.6 pmol/1E6 cells
  LMST01020010 CE(20:0) 13 13 14 pmol/1E6 cells
  LMST01020011 CE(20:1) 4.6 5.5 5.5 pmol/1E6 cells
  LMST01020012 CE(20:2) 12 13 12 pmol/1E6 cells
  LMST01020013 CE(20:3) 20 21 22 pmol/1E6 cells
  LMST01020014 CE(20:4) 58 69 67 pmol/1E6 cells
  LMST01020016 CE(22:0) 11 11 11 pmol/1E6 cells
  LMST01020025 CE(22:1) 30 36 43 pmol/1E6 cells
  LMST01020017 CE(22:2) 43 43 47 pmol/1E6 cells
  LMST01020018 CE(22:4) 31 31 31 pmol/1E6 cells
  LMST01020019 CE(22:6) 35 37 36 pmol/1E6 cells

 

*: In HPLC/MS/MS measurements by multiple reaction monitoring, the response ratio for each analyte was determined relative to a known amount of internal standard (400 pmol of 13C18 (18:1) cholesterol ester) that was added to each biological and calibration sample. The absolute amount of each analyte in a sample was then calculated by comparison to a standard curve for a given analyte. Specific six point calibration curves from 0.0 to 1.6 nmoles were constructed for each set of samples for the following cholesterol esters: 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:0, 20:1, 20:2, 20:3, 20:4, and 22:6. Analytes for which no direct reference standard was available were determined based on the curve for a standard closest in number of carbon atoms and double bonds. The value reported in the table was finally adjusted for the amount of DNA measured for each sample.