LMPD Database

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LMP003541

UniProt Annotations

Entry Information
Gene Namedolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Protein EntryOST48_HUMAN
UniProt IDP39656
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=P39656-1; Sequence=Displayed; Name=2; IsoId=P39656-2; Sequence=VSP_055498; Note=No experimental confirmation available.; Name=3; IsoId=P39656-3; Sequence=VSP_055499; Note=No experimental confirmation available.;
Catalytic ActivityDolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
CautionIt is uncertain whether Met-1 or Met-18 is the initiator.
DiseaseCongenital disorder of glycosylation 1R (CDG1R) [MIM
FunctionEssential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
InteractionQ86WV6:TMEM173; NbExp=2; IntAct=EBI-358866, EBI-2800345;
PathwayProtein modification; protein glycosylation.
Sequence CautionSequence=BAB93478.1; Type=Erroneous initiation; Evidence= ;
SimilarityBelongs to the DDOST 48 kDa subunit family.
Subcellular LocationEndoplasmic reticulum membrane; Single-pass type I membrane protein.
SubunitComponent of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome (By similarity). Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex.