LMPD Database

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LMP002953

UniProt Annotations

Entry Information
Gene Namephosphatase and tensin homolog
Protein EntryPTEN_MOUSE
UniProt IDO08586
SpeciesMouse
Comments
Comment typeDescription
Catalytic Activity[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate.
Catalytic ActivityPhosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate.
Catalytic ActivityProtein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420;
FunctionIn motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement (By similarity). Tumor suppressor. Acts as a dual- specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. {ECO:0000250, ECO:0000269|PubMed:10339565, ECO:0000269|PubMed:19778506}.
InteractionP49452:Cenpc; NbExp=2; IntAct=EBI-1186266, EBI-1186252; Q9JHL1:Slc9a3r2; NbExp=3; IntAct=EBI-1186266, EBI-538451; P02340:Tp53; NbExp=4; IntAct=EBI-1186266, EBI-474016;
PtmConstitutively phosphorylated by CK2 under normal conditions. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4 (By similarity). Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome. Phosphorylation by ROCK1 is essential for its stability and activity. {ECO:0000250, ECO:0000269|PubMed:17242355, ECO:0000269|PubMed:19473982, ECO:0000269|PubMed:20008297, ECO:0000269|PubMed:20940307}.
PtmMonoubiquitinated; monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7; leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization (By similarity). Ubiquitinated by XIAP/BIRC4. {ECO:0000250, ECO:0000269|PubMed:19473982}.
SimilarityContains 1 C2 tensin-type domain. {ECO:0000255|PROSITE-ProRule:PRU00589}.
SimilarityContains 1 phosphatase tensin-type domain. {ECO:0000255|PROSITE-ProRule:PRU00590}.
Subcellular LocationCytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Nucleus, PML body {ECO:0000250}. Note=Monoubiquitinated form is nuclear (By similarity). Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization. {ECO:0000250, ECO:0000269|PubMed:19473982}.
SubunitMonomer. The unphosphorylated form interacts with the second PDZ domain of AIP1. Interacts with MAGI2, MAGI3, MAST1 and MAST3, but neither with MAST4 nor with DLG5; interaction with MAGI2 increases protein stability (By similarity). Interacts with NEDD4. Interacts with NDFIP1 and NDFIP2; in the presence of NEDD4 or ITCH, this interaction promotes PTEN ubiquitination (By similarity). Interacts (via C2 domain) with FRK (By similarity). Interacts with USP7; the interaction is direct (By similarity). Interacts with ROCK1. Interacts with XIAP/BIRC4. Interacts with STK11; the interaction phosphorylates PTEN (By similarity). {ECO:0000250}.