Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets
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Legend: | : View data for technical replicates : Graph of technical replicates(within an expt.) : Graph of biological replicates(across expts.) |
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Details/Graph technical replicates | LM_ID | Name | Mass (neutral) |
WT-Fat WT-Chow KO-Fat KO-Chow |
Units . . . . | |
---|---|---|---|---|---|---|
(MSE070712) | LMST01020004 | CE(14:0) | 596.6 (ions) | 27 0.65 72 2.6 |
pmol/10E6 cells | |
(MSE070712) | LMST01020021 | CE(14:1) | 594.5 (ions) | 14 - 43 - |
pmol/10E6 cells | |
(MSE070712) | LMST01020005 | CE(16:0) | 624.6 (ions) | 130 39 350 79 |
pmol/10E6 cells | |
(MSE070712) | LMST01020006 | CE(16:1) | 622.6 (ions) | 210 10 330 33 |
pmol/10E6 cells | |
(MSE070712) | LMST01020024 | CE(16:2) | 620.6 (ions) | 64 1.3 100 6.2 |
pmol/10E6 cells | |
(MSE070712) | LMST01020007 | CE(18:0) | 652.6 (ions) | 81 13 150 38 |
pmol/10E6 cells | |
(MSE070712) | LMST01020003 | CE(18:1) | 650.6 (ions) | 1010 140 2120 490 |
pmol/10E6 cells | |
(MSE070712) | LMST01020008 | CE(18:2) | 648.6 (ions) | 220 27 350 110 |
pmol/10E6 cells | |
(MSE070712) | LMST01020010 | CE(20:0) | 680.6 (ions) | 16 2 12 4.7 |
pmol/10E6 cells | |
(MSE070712) | LMST01020011 | CE(20:1) | 678.6 (ions) | 13 2.2 27 6.4 |
pmol/10E6 cells | |
(MSE070712) | LMST01020012 | CE(20:2) | 676.6 (ions) | 22 3.5 46 15 |
pmol/10E6 cells | |
(MSE070712) | LMST01020014 | CE(20:4) | 672.6 (ions) | 170 28 230 160 |
pmol/10E6 cells | |
(MSE070712) | LMST01020025 | CE(22:1) | 706.7 (ions) | 9.4 1 15 3.7 |
pmol/10E6 cells | |
(MSE070712) | LMST01020019 | CE(22:6) | 696.6 (ions) | 180 11 330 70 |
pmol/10E6 cells |
*:
In HPLC/MS/MS measurements by multiple reaction monitoring, the response ratio for each analyte was determined relative to a known amount of internal standard (400 pmol of 13C18 (18:1) cholesterol ester) that was added to each biological and calibration sample. The absolute amount of each analyte in a sample was then calculated by comparison to a standard curve for a given analyte. Specific six point calibration curves from 0.0 to 1.6 nmoles were constructed for each set of samples for the following cholesterol esters: 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:0, 20:1, 20:2, 20:3, 20:4, and 22:6. Analytes for which no direct reference standard was available were determined based on the curve for a standard closest in number of carbon atoms and double bonds. The value reported in the table was finally adjusted for the amount of DNA measured for each sample.