LMPD Database

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LMP011289

UniProt Annotations

Entry Information
Gene Namesolanesyl diphosphate synthase 1
Protein EntrySPS1_ARATH
UniProt IDQ8S948
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8S948-1; Sequence=Displayed; Name=2; IsoId=Q8S948-2; Sequence=VSP_042134; Note=Derived from EST data. No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=5.73 uM for farnesyl diphosphate (in the presence of 500 uM of isopentenyl diphosphate) {ECO:0000269|PubMed:12437513}; KM=1.61 uM for geranylgeranyl diphosphate (in the presence of 100 uM of isopentenyl diphosphate) {ECO:0000269|PubMed:12437513}; KM=151 uM for isopentenyl diphosphate (in the presence of 20 uM of farnesyl diphosphate) {ECO:0000269|PubMed:12437513}; KM=20 uM for isopentenyl diphosphate (in the presence of 4 uM of geranylgeranyl diphosphate) {ECO:0000269|PubMed:12437513}; Note=kcat is 29.7 sec(-1) with farnesyl diphosphate as substrate (in the presence of 500 uM of isopentenyl diphosphate). kcat is 21.0 sec(-1) with geranylgeranyl diphosphate as substrate (in the presence of 100 uM of isopentenyl diphosphate). kcat is 14.7 sec(-1) with isopentenyl diphosphate as substrate (in the presence of 20 uM of farnesyl diphosphate). kcat is 15.0 sec(-1) with isopentenyl diphosphate as substrate (in the presence of 4 uM of geranylgeranyl diphosphate).; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:12437513};
Catalytic ActivityGeranylgeranyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-nonaprenyl diphosphate. {ECO:0000269|PubMed:12437513}.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
FunctionInvolved in the solanesyl diphosphate biosynthesis in the endoplasmic reticulum. This isoprenoid side chain might be then transferred to the mitochondria for the synthesis of ubiquinone-9. Prefers geranylgeranyl diphosphate to farnesyl diphosphate as substrate. No activity with geranyl diphosphate or dimethylallyl diphosphate as substrate. {ECO:0000269|PubMed:12437513, ECO:0000269|PubMed:15653808}.
Sequence CautionSequence=AAD30584.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the FPP/GGPP synthase family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum {ECO:0000269|PubMed:15653808, ECO:0000269|PubMed:15784989}.
SubunitHomodimer. {ECO:0000269|PubMed:12437513}.
Tissue SpecificityHigher expression in leaves than in roots. {ECO:0000269|PubMed:15784989}.