LMPD Database

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LMP010871

UniProt Annotations

Entry Information
Gene Namephospholipase D alpha 2
Protein EntryPLDA2_ARATH
UniProt IDQ9SSQ9
SpeciesArabidopsis
Comments
Comment typeDescription
Catalytic ActivityA phosphatidylcholine + H(2)O = choline + a phosphatidate.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Ca(2+) requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2- dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).;
DomainC2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands.
FunctionHydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell.
InductionActivated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses.
SimilarityBelongs to the phospholipase D family. C2-PLD subfamily. {ECO:0000305}.
SimilarityContains 1 C2 domain. {ECO:0000305}.
SimilarityContains 2 PLD phosphodiesterase domains. {ECO:0000255|PROSITE-ProRule:PRU00153}.
Subcellular LocationCytoplasm {ECO:0000269|PubMed:10198096}. Membrane {ECO:0000269|PubMed:10198096}; Peripheral membrane protein {ECO:0000269|PubMed:10198096}. Vacuole {ECO:0000269|PubMed:10198096}. Cytoplasmic vesicle, clathrin- coated vesicle {ECO:0000269|PubMed:10198096}. Note=Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated.
Tissue SpecificityHighly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds.