LMPD Database

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LMP010866

UniProt Annotations

Entry Information
Gene Namephospholipase A2-alpha
Protein EntryPLA2A_ARATH
UniProt IDQ8S8N6
SpeciesArabidopsis
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: Vmax=15.3 umol/min/mg enzyme toward Dioleolyl- phosphoethanolamine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=9.9 umol/min/mg enzyme toward DOPG (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=16.7 umol/min/mg enzyme toward Dioleolyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=19.5 umol/min/mg enzyme toward Dilinoleoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=20.6 umol/min/mg enzyme toward Dipalmitoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=27.3 umol/min/mg enzyme toward Dimyristoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=29.3 umol/min/mg enzyme toward Dilauroyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=35.5 umol/min/mg enzyme toward Didecanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=14.4 umol/min/mg enzyme toward Dioctanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=5.9 umol/min/mg enzyme toward Diheptanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; Vmax=1.1 umol/min/mg enzyme toward Dihexanoyl-phosphocholine (in the presence of 10 mM Calcium) {ECO:0000269|PubMed:17692835}; pH dependence: Optimum pH is 8.5-9. {ECO:0000269|PubMed:17692835}; Temperature dependence: Optimum temperature is 30-40 degrees Celsius. {ECO:0000269|PubMed:17692835};
Catalytic ActivityPhosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate. {ECO:0000255|PROSITE- ProRule:PRU10035, ECO:0000269|PubMed:16140037}.
CofactorName=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 1 Ca(2+) ion per subunit.;
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Releases lysophospholipids (LPLs) and free fatty acids (FFAs) from membrane phospholipids in response to hormones and other external stimuli. Modulates the trafficking of PIN proteins to the plasma membrane. {ECO:0000269|PubMed:16140037, ECO:0000269|PubMed:20525850}.
MiscellaneousThe enzyme has a preference towards linoleoyl acyl chain over palmitoyl acyl chain. It also has a slight preference for phosphatidylethanolamine over phosphatidylcholine.
SimilarityBelongs to the phospholipase A2 family. {ECO:0000305}.
Subcellular LocationSecreted {ECO:0000250}. Golgi apparatus {ECO:0000269|PubMed:20525850}.
Tissue SpecificityUbiquitous but expressed at a low level. {ECO:0000269|PubMed:15748654, ECO:0000269|PubMed:16140037}.