LMPD Database

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LMP009902

UniProt Annotations

Entry Information
Gene Namebeta-carotene 3-hydroxylase 1
Protein EntryBCH1_ARATH
UniProt IDQ9SZZ8
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9SZZ8-1; Sequence=Displayed; Name=2; IsoId=Q9SZZ8-2; Sequence=VSP_041793, VSP_041794; Note=Derived from EST data. No experimental confirmation available.;
Catalytic ActivityBeta-carotene + 2 NADH + 2 O(2) = zeaxanthin + 2 NAD(+) + 2 H(2)O. {ECO:0000269|PubMed:8798688}.
Disruption PhenotypeNo visible phenotype when grown under normal light conditions; due to redundancy with BCH2. Bch1 and bch2 double mutant has no visible phenotype but lower levels of beta, beta-xanthophylls and increased beta-carotene and lutein. Cyp97c1, bch1 and bch2 triple mutant is paler and smaller than wild-type. {ECO:0000269|PubMed:12782726, ECO:0000269|PubMed:16890225}.
DomainThe histidine box domains may contain the active site and/or be involved in iron binding.
DomainThe N-terminal part of the mature protein (70-129) is probably involved in the formation of a functional homodimer.
FunctionNonheme diiron monooxygenase involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylations of beta-carotene. Has also a low activity toward the beta- and epsilon-rings of alpha-carotene. No activity with acyclic carotenoids such as lycopene and neurosporene. Uses ferredoxin as an electron donor (Probable). {ECO:0000305|PubMed:12782726, ECO:0000305|PubMed:16890225, ECO:0000305|PubMed:19147649, ECO:0000305|PubMed:8798688}.
MiscellaneousA protein lacking the first 69 amino acids has a normal in vitro activity, while a protein lacking 129 N-terminal amino acids produces predominantly the monohydroxy intermediate beta-cryptoxanthin.
SimilarityBelongs to the sterol desaturase family. {ECO:0000305}.
Subcellular LocationPlastid, chloroplast membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
SubunitHomodimer. {ECO:0000305}.
Tissue SpecificityExpressed in leaves, flowers, stems, roots and siliques. {ECO:0000269|PubMed:11587509}.