LMPD Database

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LMP007631

UniProt Annotations

Entry Information
Gene Name2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Protein EntryISPF_ECOLI
UniProt IDP62617
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=339 uM for CDP-ME2P (at pH 7.4) {ECO:0000269|PubMed:22839733}; Note=kcat is 61 min(-1) for CDP-ME2P (at pH 7.4).;
Catalytic Activity2-phospho-4-(cytidine 5'-diphospho)-2-C- methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. {ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:22839733}.
CofactorName=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:11786530, ECO:0000269|PubMed:11829504, ECO:0000269|PubMed:11997478, ECO:0000269|PubMed:15608374, ECO:0000269|PubMed:16392111, ECO:0000269|PubMed:16511114, ECO:0000269|PubMed:19320487}; Note=Binds 1 divalent metal cation per subunit. {ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:11786530, ECO:0000269|PubMed:11829504, ECO:0000269|PubMed:11997478, ECO:0000269|PubMed:15608374, ECO:0000269|PubMed:16392111, ECO:0000269|PubMed:16511114, ECO:0000269|PubMed:19320487};
Disruption PhenotypeCells lacking this gene reveal a filamentous phenotype. {ECO:0000269|PubMed:12270818}.
Enzyme RegulationActivated by 2C-methyl-D-erythritol 4-phosphate (MEP). {ECO:0000269|PubMed:22839733}.
FunctionInvolved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). Also converts 4-diphosphocytidyl-2-C-methyl-D-erythritol into 2-C- methyl-D-erythritol 3,4-cyclophosphate and CMP. {ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:22839733}.
InteractionP67910:hldD; NbExp=1; IntAct=EBI-562321, EBI-543760;
PathwayIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 4/6.
SimilarityBelongs to the IspF family. {ECO:0000305}.
SubunitHomotrimer. {ECO:0000269|PubMed:11786530, ECO:0000269|PubMed:11829504, ECO:0000269|PubMed:11997478, ECO:0000269|PubMed:15608374, ECO:0000269|PubMed:16392111, ECO:0000269|PubMed:16511114, ECO:0000269|PubMed:19320487}.