LMPD Database

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LMP007615

UniProt Annotations

Entry Information
Gene Namelong-chain acyl-CoA thioesterase III
Protein EntryFADM_ECOLI
UniProt IDP77712
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=3.0 uM for 3,5-cis-tetradecadienoyl-CoA {ECO:0000269|PubMed:18576672}; KM=10.8 uM for 3,5-cis-dodecadienoyl-CoA {ECO:0000269|PubMed:18576672}; KM=5.8 uM for 3-hydroxytetradecanoyl-CoA {ECO:0000269|PubMed:18576672}; KM=6.0 uM for palmitoyl-CoA {ECO:0000269|PubMed:18576672}; KM=6.3 uM for myristoyl-CoA {ECO:0000269|PubMed:18576672}; KM=13.0 uM for lauroyl-CoA {ECO:0000269|PubMed:18576672}; Vmax=101.3 umol/min/mg enzyme with 3,5-cis-tetradecadienoyl-CoA as substrate {ECO:0000269|PubMed:18576672}; Vmax=92.3 umol/min/mg enzyme with 3,5-cis-dodecadienoyl-CoA as substrate {ECO:0000269|PubMed:18576672}; Vmax=45.5 umol/min/mg enzyme with 3-hydroxytetradecanoyl-CoA as substrate {ECO:0000269|PubMed:18576672}; Vmax=43.5 umol/min/mg enzyme with palmitoyl-CoA as substrate {ECO:0000269|PubMed:18576672}; Vmax=26.4 umol/min/mg enzyme with myristoyl-CoA as substrate {ECO:0000269|PubMed:18576672}; Vmax=6.9 umol/min/mg enzyme with lauroyl-CoA as substrate {ECO:0000269|PubMed:18576672};
FunctionLong-chain acyl-CoA thioesterase with a preference for 3,5-tetradecadienoyl-CoA. Could be involved in beta-oxidation of fatty acids. {ECO:0000269|PubMed:18576672}.
InductionUp-regulated by growth on oleic acid. Repressed by FadR and by the cyclic AMP receptor protein-cAMP (CRP-cAMP) complex. {ECO:0000269|PubMed:18576672, ECO:0000269|PubMed:19684132}.
SimilarityBelongs to the 4-hydroxybenzoyl-CoA thioesterase family. {ECO:0000305}.
SubunitHomotetramer. {ECO:0000305}.