LMPD Database

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LMP007574

UniProt Annotations

Entry Information
Gene Name1-deoxy-D-xylulose 5-phosphate reductoisomerase
Protein EntryDXR_ECOLI
UniProt IDP45568
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=250 uM for DXP {ECO:0000269|PubMed:10787409}; Note=Measured in the presence of manganese ions.; pH dependence: Optimum pH is 7.0-8.5. {ECO:0000269|PubMed:10787409}; Temperature dependence: Optimum temperature is 40-60 degrees Celsius. {ECO:0000269|PubMed:10787409};
Catalytic Activity2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH.
CofactorName=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Note=Divalent cation. Prefers Mg(2+), Mn(2+) or Co(2+).;
Enzyme RegulationInhibited by fosmidomycin.
FunctionCatalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).
PathwayIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 1/6.
SimilarityBelongs to the DXR family. {ECO:0000305}.
SubunitHomodimer. {ECO:0000269|PubMed:11741911, ECO:0000269|PubMed:11872159, ECO:0000269|PubMed:12621040, ECO:0000269|PubMed:15339150, ECO:0000269|PubMed:15567415}.